A NovelN⁶-Substituted Adenosine Isolated from Mi Huan Jun (Armillaria mellea) as a Cerebral-Protecting Compound

Abstract

Successive purification of a crude extract of cultured Mi Huan Jun mycelia, followed by an assay of the effect on complete ischemia in mice, led to the isolation of a new compound with cerebral protecting activity, hereafter designated as AMG-1. The structure of AMG-1 was proposed as being 6-(5-hydroxy-2-pyridyl-methylamino)-9-beta-ribofuranosylpurine (1) on the basis of its UV, mass, 1H-NMR, and 13C-NMR spectra.

Ligusticum chuanxiong as a potential neuroprotectant for preventing serum deprivation-induced apoptosis in rat pheochromocytoma cells: Functional roles of mitogen-activated protein kinases

“…5B), it is possible that ADO-based derivatives may exist in the LC-BuOH. In other plants for example, a novel N 6 -substituted adenosine isolated from Armillaria mellea has cerebral-protective effects (Watanabe et al, 1990). Also, an N 6 -(4-hydroxybenzyl)adenosine derived from Gastrodia elata was protective in this system .…”

Adenosine as an active component of Antrodia cinnamomea that prevents rat PC12 cells from serum deprivation-induced apoptosis through the activation of adenosine A2A receptors

“…This viewpoint could be extended by the following findings. A novel N 6 -substituted adenosine isolated from Armillaria mellea has cerebroprotective effects (Watanabe et al, 1990). Several adenosine derivatives derived from Atriolum robustum are shown to inhibit cyclic-AMP formation through A 1 -R activation (Kehraus et al, 2004).…”

Protective effects of the novel adenosine derivative WS0701 in a mouse model of posttraumatic stress disorder

“…AMG-1 is an N6 substituted adenosine derivative extracted from Armillaria mellea, which is a traditional Chinese medicine. Previous research has shown that AMG-1 has neuroprotective [8] and anticonvulsant effects [9] that are related to reductions in cellular energy metabolism [10] and glutamate release [11] and inhibition of increases in intracellular calcium [12] . {(2R,3S,4R,5R)-3,4-dihydroxy-5-[6-[(4-hydroxy-3-methoxybenzyl)amino]-9H-purin-9-yl]tetrahydrofuran-2-yl} methyl decanoate (WS0701), the structure of which has been confirmed by 1 H-NMR and ESI-MS [13] (Figure 1), is a modified form of AMG-1 that was created to increase the sedative effect of AMG-1.…”