A novel hydrogen peroxide sensor based on the direct electron transfer of horseradish peroxidase immobilized on silica–hydroxyapatite hybrid film

Wang, Bin; Zhang, Jingjing; Pan, Zhuang-Ying; Tao, Xuquan; Wang, Huaisheng

doi:10.1016/j.bios.2008.06.053

Cited by 143 publications

(38 citation statements)

References 40 publications

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“…Therefore, several calcium orthophosphate-based biocomposites and hybrid biomaterials were found to be applicable for biosensor manufacturing. 354,642,1041,1083,[1122][1123][1124][1125][1126][1127] For example, formation of poly-l-lysine/HA/carbon nanotube hybrid nanodimensional particles was described, and a general design strategy for an immunosensing platform was proposed based on adsorption of antibodies onto this biocomposite. 1083 In another paper, a hybrid material formed by assembling nanodimensional particles of gold onto nano-sized HA was employed for the interface design of a piezoelectric immunosensor on which the antibodies were bound.…”

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tmentioning

confidence: 99%

Biocomposites and hybrid biomaterials based on calcium orthophosphates

Dorozhkin¹

2011

Biomatter

152

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Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tmentioning

confidence: 99%

Biocomposites and hybrid biomaterials based on calcium orthophosphates

Dorozhkin¹

2011

Biomatter

152

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“…Figure 4 presents the Nyquist plots of the impedance spectroscopy of the bare and modified ITO electrodes in the frequency range of 10 À1 to 10 4 Hz. The diameter of the semicircle for CH deposited ITO electrode (curve b) is much larger than the bare ITO electrode (curve a), suggesting that the CH film formed on the surface of ITO hinders the electron transfer between the redox probe of [Fe(CN) 6 ] 3À / 4À and the electrode surface. When the GFe 3 O 4 composites were embedded in CH to modified ITO electrode, the diameter decreased markedly (curve c).…”

Section: Electrochemical Studies Of the Biosensormentioning

confidence: 95%

Preparation and Application of Mediator‐Free H₂O₂ Biosensors of Graphene‐Fe₃O₄ Composites

et al. 2011

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Graphene-Fe 3 O 4 (G-Fe 3 O 4 ) composites were prepared with a solvothermal method for the immobilization of horseradish peroxidase (HRP) to construct a mediator-free H 2 O 2 biosensor. The morphology and structure of the GFe 3 O 4 composites were analyzed by TEM, SEM, and XRD. Chitosan (CH) was utilized to disperse the composites, and the mixture of CH, G-Fe 3 O 4 and HRP was spin-coated on an indium tin oxide (ITO) electrode to form a biocomposites film. Electrochemical impedance spectroscopy (EIS) and cyclic voltammograms (CV) demonstrated the fast electron transfer process between HRP and the electrode. The resulted H 2 O 2 biosensor exhibited a rapid response upon the addition of H 2 O 2 , a high sensitivity of 132 mA mM À1 cm À2 and a low detection limit of 6 10 À7 M. The linear range of the biosensor is from 5 10 À6 to 3.81 10 À3 M (r = 0.997). Such interesting G-Fe 3 O 4 composites with easy preparation and low cost can provide a novel matrix for enzyme immobilization to construct biosensors with higher performance.

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“…As far as we know, the lower detection limit is better than those of other DET-type HRP enzyme electrodes based on CNTs [13][14][15] and other materials. 28,29 Compared to bare Pt electrode, which exhibits potential detection at +0.60 V (vs. Ag | AgCl), this electrode seems to be able to detect the lower concentrations of hydrogen peroxide. However, a Pt electrode needs a variety of surface modifications for selective detection of hydrogen peroxide as described above.…”

Section: Hrp/cnts/gc Electrodes For Hydrogen Peroxide Detectionmentioning

confidence: 99%

Nano-Molar Level Hydrogen Peroxide Detection by Horseradish Peroxidase Adsorbed Cup-Stacked Carbon Nanotube Electrodes and Applications to l-Glutamate Detection

2010

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We have developed a simple fabrication method of a highly sensitive direct electron transfer-type electrochemical biosensor for hydrogen peroxide by use of cup-stacked carbon nanotubes (CSCNTs). The CSCNTs, formed by stacking of cup-shaped carbon units, has larger internal space and more hydrophilic edges, thanks to the presence of functional groups containing oxygen (e.g., -COOH, -OH), than multi-walled carbon nanotubes (MWCNTs). When the CSCNTs suspension was cast, the CSCNTs were dispersed homogeneously onto a glassy carbon (GC) electrode, and horseradish peroxidase (HRP) was immobilized firmly by physical adsorption without any chemical reactions. The flow injection analysis (FIA) system with the HRP/CSCNTs/GC electrode has superior sensitivity and stability to the HRP/MWCNTs/GC electrode. The detection limit was 0.75 nM (S/N = 3) and the activity was maintained over 85% for 21 days. Further, when the glutamate oxidase (GlOD)-immobilized reactor was set into the proposed FIA system, L-glutamate could be detected repeatedly with a detection limit of 1.2 nM (S/N = 3).

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A novel hydrogen peroxide sensor based on the direct electron transfer of horseradish peroxidase immobilized on silica–hydroxyapatite hybrid film

Cited by 143 publications

References 40 publications

Biocomposites and hybrid biomaterials based on calcium orthophosphates

Biocomposites and hybrid biomaterials based on calcium orthophosphates

Preparation and Application of Mediator‐Free H₂O₂ Biosensors of Graphene‐Fe₃O₄ Composites

Nano-Molar Level Hydrogen Peroxide Detection by Horseradish Peroxidase Adsorbed Cup-Stacked Carbon Nanotube Electrodes and Applications to l-Glutamate Detection

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A novel hydrogen peroxide sensor based on the direct electron transfer of horseradish peroxidase immobilized on silica–hydroxyapatite hybrid film

Cited by 143 publications

References 40 publications

Biocomposites and hybrid biomaterials based on calcium orthophosphates

Biocomposites and hybrid biomaterials based on calcium orthophosphates

Preparation and Application of Mediator‐Free H2O2 Biosensors of Graphene‐Fe3O4 Composites

Nano-Molar Level Hydrogen Peroxide Detection by Horseradish Peroxidase Adsorbed Cup-Stacked Carbon Nanotube Electrodes and Applications to l-Glutamate Detection

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Preparation and Application of Mediator‐Free H₂O₂ Biosensors of Graphene‐Fe₃O₄ Composites