Nano-Molar Level Hydrogen Peroxide Detection by Horseradish Peroxidase Adsorbed Cup-Stacked Carbon Nanotube Electrodes and Applications to l-Glutamate Detection

Noda, Tatsuo; Ukai, Tadao; Yao, Takeshi

doi:10.2116/analsci.26.675

Cited by 8 publications

(3 citation statements)

References 38 publications

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“…However, the insulating PLL and PSS prevent efficient electrochemical communication between HRP and CSCNFs and its kinetics study. Noda and co-workers previously developed a flow injection analysis system combined with HRP-adsorbed CSCNF electrodes for H 2 O 2 sensing, but they did not studied the reaction kinetics . Here we addressed this issue on the basis of a technique reported by Yan and co-workers, in which redox proteins were adsorbed onto carbon nanotubes with surfactant sodium dodecyl sulfate (SDS) for facile direct communication.…”

Section: Introductionmentioning

confidence: 99%

“…Noda and co-workers previously developed a flow injection analysis system combined with HRP-adsorbed CSCNF electrodes for H 2 O 2 sensing, but they did not studied the reaction kinetics. 23 Here we addressed this issue on the basis of a technique reported by Yan and coworkers, 24 in which redox proteins were adsorbed onto carbon nanotubes with surfactant sodium dodecyl sulfate (SDS) for facile direct communication. We immobilized HRP at the surface of CSCNFs with SDS and improved the efficiency of the electrochemical communication by 2 orders of magnitude.…”

Section: ■ Introductionmentioning

confidence: 99%

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Direct Electron Transfer Kinetics of Peroxidase at Edge Plane Sites of Cup-Stacked Carbon Nanofibers and Their Comparison with Single-Walled Carbon Nanotubes

2016

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Electron transfer kinetics at the graphene edge site is of great interest from the viewpoints of application to sensing and energy conversion and storage. Here we analyzed kinetics of direct electron transfer of horseradish peroxidase (HRP) adsorbed through surfactant sodium dodecyl sulfate at cup-stacked carbon nanofibers (CSCNFs), which provide highly ordered graphene edges, and compared it with that at single-walled carbon nanotubes (SWCNTs), which consist of a rolled-up basal plane graphene. The heterogeneous electron transfer rate constant of the Fe(2+/3+) couple of the HRP reaction center at CSCNFs (ca. 34.8 s(-1)) was an order of magnitude larger than that at SWCNTs (ca. 4.7 s(-1)). In addition, the overall rate constant of the electron transfer reaction from CSCNFs to HRP oxidized by H2O2 was higher than that from SWCNTs by a factor of 3. CSCNFs also allowed enhancement of the complex-formation reaction rate of HRP with H2O2, in comparison with that at SWCNTs. CSCNFs would therefore be applied to not only biosensors but also biofuel cells with enhanced performance.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Direct Electron Transfer Kinetics of Peroxidase at Edge Plane Sites of Cup-Stacked Carbon Nanofibers and Their Comparison with Single-Walled Carbon Nanotubes

2016

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“…Noda et al have developed hydrogene peroxide sensors based on CSCNF and horseradish peroxidase (HRP). 18 However, the nature of the CSCNF used and kinetics of the electrochemical reactions have not yet been studied extensively.…”

Section: Introductionmentioning

confidence: 99%

Peroxidase-modified cup-stacked carbon nanofiber networks for electrochemical biosensing with adjustable dynamic range

Takahashi

Fujita

et al. 2012

RSC Adv.

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Cup-stacked carbon nanofibers (CSCNFs) were treated with ozone to introduce oxygen-containing groups to the edges of the graphene cups. The O/C atomic ratio at the surface was estimated to be 5.3 6 10 22 . The hydrophilic CSCNFs were used for constructing a three-dimensional network that works both as an electrical nanowire and an enzyme support. Horseradish peroxidase (HRP) molecules were immobilized onto the network for amperometric sensing of H 2 O 2 and inhibitionbased sensing of cyanide. Both the upper sensing limit of H 2 O 2 and the upper and lower sensing limits of cyanide were controlled by about two orders of magnitude by changing the HRP coverage.

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Nanomolar Detection of Glutamate at a Biosensor Based on Screen‐Printed Electrodes Modified with Carbon Nanotubes

2011

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The amperometric glutamate biosensor based on screen-printed electrodes containing carbon nanotubes (CNT), and its integration in a flow injection analysis system, is described herein. The sensor was fabricated by simply adsorbing enzyme glutamate oxidase (GlutOx) on a commercial substrate containing multi-wall CNT. The resulting device displayed excellent electroanalytical properties toward the determination of L-glutamate in a wide linear range (0.01–10 μM) with low detection limit (10 nM, S/N≥3), fast response time (≤5 s), and good operational and long-term stability. The CNT modified screen-printed electrodes have a potential to be of general interest for designing of electrochemical sensors and biosensors.

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Nano-Molar Level Hydrogen Peroxide Detection by Horseradish Peroxidase Adsorbed Cup-Stacked Carbon Nanotube Electrodes and Applications to l-Glutamate Detection

Cited by 8 publications

References 38 publications

Direct Electron Transfer Kinetics of Peroxidase at Edge Plane Sites of Cup-Stacked Carbon Nanofibers and Their Comparison with Single-Walled Carbon Nanotubes

Direct Electron Transfer Kinetics of Peroxidase at Edge Plane Sites of Cup-Stacked Carbon Nanofibers and Their Comparison with Single-Walled Carbon Nanotubes

Peroxidase-modified cup-stacked carbon nanofiber networks for electrochemical biosensing with adjustable dynamic range

Nanomolar Detection of Glutamate at a Biosensor Based on Screen‐Printed Electrodes Modified with Carbon Nanotubes

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