A novel hydrogen peroxide biosensor based on horseradish peroxidase immobilized on gold nanoparticles–silk fibroin modified glassy carbon electrode and direct electrochemistry of horseradish peroxidase

Direct electrochemistry of horseradish peroxidase immobilized on a monolayer modified nanowire array electrode

“…3C inset, the anodic or cathodic peak current was proportional to the scan rate, as I pa ( A) = 1.0488 + 113.7v(V s −1 ) (R = 0.9987) and I pc ( A) =−1.0063 − 113.84v(V s −1 )( R = 0.9984). This further suggested the electrochemical behaviour of HRP on the NAEs was typical of a surface-controlled quasi-reversible process (Yin et al, 2009), verifying that immobilized HRP was stable on the NAEs.…”

“…The K app M values of HRP/NAE 2h , HRP/NAE 4h and HRP/NAE 5h were 1.87, 1.54 and 0.64 mM, respectively, significantly lower than reported values in Table 2. These results indicated that the immobilized HRP possessed high enzymatic activity and the HRP/NAEs exhibited high affinity for H 2 O 2 (Yin et al, 2009).…”

“…In comparison to the 2D approach, entrapment/encapsulation provides a 3D porous structure which drastically increases the active sites for enzyme immobilization. Further improvements have been reported by combining nanostructures with SAM and/or entrapment (Kang et al, 2009;Yin et al, 2009). However, the distribution of enzyme in the gel or the polymer 3D matrix is usually not uniform, sometimes spatially hindered, resulting in relatively low electron transfer efficiency because only a very small percentage of the enzyme immobilized is electroactive.…”

Bienzymatic glucose biosensor based on direct electrochemistry of cytochrome c on gold nanoparticles/polyaniline nanospheres composite

“…4, inset), a detection limit of 0.003 mM was estimated at the signal-to-noise ratio of 3. The modified electrode displays a wider linear range and lower detection limit than those reported in literature [21]. The excellent electrocatalytic activity of the electrode toward H 2 O 2 also means that the electrode can represent a new electrochemical platform that provides operational access to a large number of oxidase-based enzymes for fabricating bienzyme biosensors.…”

Nano-Molar Level Hydrogen Peroxide Detection by Horseradish Peroxidase Adsorbed Cup-Stacked Carbon Nanotube Electrodes and Applications to l-Glutamate Detection

“…As far as we know, the lower detection limit is better than those of other DET-type HRP enzyme electrodes based on CNTs [13][14][15] and other materials. 28,29 Compared to bare Pt electrode, which exhibits potential detection at +0.60 V (vs. Ag | AgCl), this electrode seems to be able to detect the lower concentrations of hydrogen peroxide. However, a Pt electrode needs a variety of surface modifications for selective detection of hydrogen peroxide as described above.…”