A novel HPLC method for determination of EDTA in a cataract inhibiting ophthalmic drug

Flow Injection Chemiluminescence Determination of EDTA in Canned Food

“…Gas chromatographic methods (Sorvari et al 1996;Nishikawa and Okumura 1995;Retho and Diep 1989) always include a time-consuming derivatization step, in which EDTA is converted into methyl, ethyl, propyl, or butyl esters to obtain volatility. Similarly, HPLC methods are mainly based on a precolumn derivatization step in which complexation of EDTA with Cu 2þ or Fe 3þ is conducted (Jong et al 1991;Kord et al 1995;Zhao et al 2001;Pistos and Poulou 2002;Gucht 1994), while postcolumn derivatization for the formation of fluorescent ternary complexes has also been reported (Ye and Lucy 1996).…”

“…Levels of EDTA commonly found in canned food are in the range of 100-300 ppm (Concon 1988). In pharmaceutical formulations it enhances the action of preservatives and antibacterial and stabilizes the action of antioxidants (Jong, Polanen, and Driessen 1991;Kord, Tumanova, and Matier 1995). In personal care products, it is added to cosmetics to improve their stability in air (Lanigan and Yamarik 2002).…”

Development and validation of a method for the determination of EDTA in non-alcoholic drinks by HPLC

“…The commonly used methodologies for EDTA quantification in different types of samples (pharmaceutical forms, drinking waters, sauces, mayonnaises and others) are based upon the complex formation of EDTA with copper (Kord et al, 1995;Pistos and Parissi-Poulou, 2002) or Iron salts (Shimadzu, 2005;Fidler Meredith, 2003;Yamaguchi et al, 1983). Some authors use DAD detectors (Fidler Meredith, 2003: Yamaguchi et al, 1983 and other use UV detectors (Kord et al, 1995;Pistos and ParissiPoulou, 2002;Shimadzu, 2005).…”

“…Some authors use DAD detectors (Fidler Meredith, 2003: Yamaguchi et al, 1983 and other use UV detectors (Kord et al, 1995;Pistos and ParissiPoulou, 2002;Shimadzu, 2005). In the present study, as it was necessary to determine EDTA in non-alcoholic beverages, a method based on an isocratic HPLC system, using a C18 reverse-phase column and a UV detector was developed.…”

Highly Sensitive Determination of Ethylenediaminetetraacetic Acid Using a Permanganate Chemiluminescence System Catalyzed by Gold Nanoparticles

“…4 It is also added to pharmaceuticals to enhance the action of preservatives and antibacterials and to stabilize the action of antioxidants. 3,5 These properties are due to its ability to chelate metal ions, which decrease their reactivity and prevent them to catalyze oxidation reactions, as well as to inactivate enzymes that require di-or trivalent cations for activity. 3 Disodium edetate is, moreover, used as an anticoagulant since, it can chelate calcium and prevent the coagulation of blood in vitro.…”