A novel highly flexible, simple, rapid and low-cost fabrication tool for paper-based microfluidic devices (μPADs) using technical drawing pens and in-house formulated aqueous inks

Nuchtavorn, Nantana; Macka, Mirek

doi:10.1016/j.aca.2016.03.018

Cited by 78 publications

(45 citation statements)

References 38 publications

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“…35,36 The radical scavenging activity of the antioxidant has also been measured using the DPPH assay on μPADs. However, only two antioxidant standards, including gallic acid 38 and butylated hydroxytoluene 39 have been investigated. No further investigation on the optimization of the assay as well as the stability of the developed devices has been carried out.…”

Section: Introductionmentioning

confidence: 99%

“…No further investigation on the optimization of the assay as well as the stability of the developed devices has been carried out. 38,39 Moreover, the calibration curve for DPPH assay was generated using μPADs that have different features to those used for sample analysis, which could potentially affect the method accuracy. 39 Here, we present a fully investigated study of a paper-based DPPH assay for low cost, simple to operate, rapid, low reagent consumption and high throughput analysis of antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

“…38,39 Moreover, the calibration curve for DPPH assay was generated using μPADs that have different features to those used for sample analysis, which could potentially affect the method accuracy. 39 Here, we present a fully investigated study of a paper-based DPPH assay for low cost, simple to operate, rapid, low reagent consumption and high throughput analysis of antioxidant activity. The μPAD was fabricated by the lamination method, and the DPPH reagent was deposited on the detection zone of the device, and allowed to dry.…”

Section: Introductionmentioning

confidence: 99%

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Paper-based DPPH Assay for Antioxidant Activity Analysis

2018

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We report on a paper-based 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) assay for a simple, inexpensive, low reagent and sample consumption and high throughput analysis of antioxidant activity. The paper-based device was fabricated using a lamination method to create a 5-mm in diameter circular test zone that was embedded with a DPPH reagent. The analysis was carried out in one-step by dropping an antioxidant/sample onto the test zone. After reduction by the antioxidant, the DPPH radicals become stable DPPH molecules, resulting in a change in color from deep violet to pale yellow. The violet color intensity of DPPH was inversely proportional to the antioxidant activity of the samples, and was measured using imaging software. A high precision and a low limit of detection were found in the analysis of six standard antioxidants including gallic acid, trolox, ascorbic acid, caffeic acid, vanilliic acid and quercetin. The device was then validated against the traditional spectrophotometric DPPH assay by analyzing the antioxidant activity of 7 tea samples. The results showed no significant difference for gallic acid equivalent for all 7 samples obtained from the two methods at the 95% confidence level, indicating that the developed method was reliable for antioxidant activity analysis of real samples. Finally, the paper-based DPPH device was found to be stable over 10 days when stored in a refrigerator (2 - 4°C), making it an easy-to-use device for end-users.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Paper-based DPPH Assay for Antioxidant Activity Analysis

2018

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“…The fabricated PADs could be utilized for colorimetric measurements of flavonoid, phenolic compounds, and DPPH (i.e. 2,2‐diphenyl‐1‐picrylhydrazyl) free radical scavenging activity . Later, this technique was characterized for high‐throughput deposition of chemical ink on PADs and the versatility of the pen‐plotting deposition method was shown in trace analysis of aluminium in natural water .…”

Section: Fabrication and Detection Techniquesmentioning

confidence: 99%

Recent applications of paper‐based point‐of‐care devices for biomarker detection

Suntornsuk

2019

Electrophoresis

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Detection of biomarkers is essential for diagnosis and prognosis of diseases for healthcare teams to provide timely appropriate treatments. Reliable, inexpensive, and sensitive devices are desirable to serve this purpose. Paper‐based analytical devices (PADs) have gained enormous attention during the past decade as point‐of‐care devices for biomarker detection due to their simplicity, portability, and biocompatibility. Importantly, the devices highly comply with the WHO “ASSURED” concept (i.e. Affordable, Sensitive, Specific, User‐friendly, Rapid and Robust, Equipment free, and Deliverable to end‐users). Thus, PADs could be sustainably alternative tools for biomarker detection, especially in resources‐constraint areas where budget, skillful operators, and health infrastructure are limited. First, this review introduces overviews of biomarkers, their detection and brief history of PADs. Second, description of common fabrication and detection techniques in PADs are provided. Then, it highlights recent state of the art technologies and applications of PADs for various biomarker detection published during 2018 to May 2019. Applications for tumor marker, cardiac and coronary heath disease, and metabolic disorder biomarker detection are tabulated and selected examples are described in details. Finally, it discusses the current challenges, advances, and novel applications of PADs. The review would be valuable for healthcare workers using PADs as cost‐effective point‐of‐care devices for biomarker detection and for researchers working on future developments of the devices.

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“…Water composition testing is one of the most needed analyses, especially in developing countries where water quality is poor. The parameters of this analysis, such as metals, organic pollutants, pesticides, bacteria and viruses, and other toxic substances, are monitored mostly using instrumental methods (e.g., GC, spectrometry, potentiometry, conductimetry, AAS, ICP-MS, immunoassays, and the like) but also microfluidics [12][13][14][15][16][17][18][19][20][21]. Some examples of microfluidic water analysis using smartphone can be mentioned here.…”

Section: Paper-based Devicesmentioning

confidence: 99%

Smartphones & microfluidics: Marriage for the future

Hárendarčíková

Petr

2018

Electrophoresis

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Smartphones have become widely recognized as a very interesting detection and controlling tool in microfluidics. They are portable devices with built-in cameras and internal microprocessors which carry out image processing. In this case, the external computers are not needed and phones can provide fast and accurate results. Moreover, the connectivity of smartphones gives the possibility to share and provide real-time results when needed, whether in health diagnostics, environmental monitoring, immunoassays or food safety. Undoubtedly, the marriage of smartphones and microfluidics has a brilliant future in building low cost and easily operable systems for analysis in the field, realizing the idea of people's "smartlife". The aim of this review is to present and summarize the main advantages and disadvantages of the use of smartphones as well as to take a closer look at some novel achievements published during the last couple of years. In the next paragraphs, readers will find specific uses of a combination of smartphones and microfluidics such as water analysis, health analysis (virus and bacteria detection), and measurement of physical properties or smartphone liquid control in polymer devices.

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A novel highly flexible, simple, rapid and low-cost fabrication tool for paper-based microfluidic devices (μPADs) using technical drawing pens and in-house formulated aqueous inks

Cited by 78 publications

References 38 publications

Paper-based DPPH Assay for Antioxidant Activity Analysis

Paper-based DPPH Assay for Antioxidant Activity Analysis

Recent applications of paper‐based point‐of‐care devices for biomarker detection

Smartphones & microfluidics: Marriage for the future

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