A novel graphene oxide amplified fluorescence anisotropy assay with improved accuracy and sensitivity

Abstract

In this contribution, a novel and versatile graphene oxide (GO) amplified fluorescence anisotropy (FA) strategy with improved accuracy and sensitivity for the detection of a panel of molecules, single-stranded DNA (ssDNA), adenosine and thrombin, has been successfully developed.

A self-assembling RNA aptamer-based graphene oxide sensor for the turn-on detection of theophylline in serum

“…Fluorescence anisotropy (FA) measurement provides the rotational motion of fluorescently labeled molecules in its microenvironment; thus, FA values depend on the molecular volume and local movement of fluorescent molecules (Xiao et al, 2015). In order to understand the interactions among FAM-labeled TA1, TA2, theophylline and GO in solution, the FA of FAM-labeled TA1 under different conditions (in 50 mM HEPES buffer with 150 mM NaCl and 1 mM MgCl 2 ) was measured (detailed steps are shown in the Supplementary materials).…”

Graphene-Based Biosensors for Detection of Biomarkers

“…The presence of target ATP induced the release of dye-labeled ssDNA, which bound to GO to amplify the mass, thus leading to an increased anisotropy signal as shown in Figure 4B. Xiao et al reported a novel GO amplified FA sensor for the quantitative detection of a variety of target molecules (ssDNA, adenosine and thrombin) [55]. Dye-labeled-ssDNA was immobilized on GO through a double-stranded region with the capture DNA, and such a design allowed an appropriate distance between GO and fluorescent dye, which restricted the rotation of dye with the entire formation to get a high FA signal and decreased the quenching efficiency of GO towards the dye.…”

Graphene-Based Biosensors for Detection of Biomarkers

“…The presence of target ATP induced the release of dye-labeled ssDNA, which bound to GO to amplify the mass, thus leading to an increased anisotropy signal as shown in Figure 3B. Xiao et al reported a novel GO amplified FA sensor for the quantitative detection of a variety of target molecules (ssDNA, adenosine and thrombin) [29]. Dye labeled-ssDNA was immobilized on GO through doublestranded region with the capture DNA, such a design allowed an appropriate distance between GO and fluorescent dye, which restricted the rotation of dye with the entire formation to get a high FA signal and decreased the quenching efficiency of GO toward dye.…”