A novel glycerophosphodiesterase from <i>Bacillus pumilus</i>

Kusser, Wolfgang; Fiedler, F.

doi:10.1016/0014-5793(84)80100-3

Cited by 10 publications

(4 citation statements)

References 27 publications

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“…(17). Such activities have also been reported before in Bacillus (18) and in some bacteriophages (19 -21), but the enzymes have hardly been isolated and never before described in staphylococci. Thus, S. aureus GlpQ and the recently described homologous enzyme from B. subtilis are the first identified bacterial teichoicases important for phosphate supply.…”

supporting

confidence: 55%

Staphylococcus aureus counters phosphate limitation by scavenging wall teichoic acids from other staphylococci via the teichoicase GlpQ

Jorge

Schneider

Unsleber

et al. 2018

Journal of Biological Chemistry

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is part of the human nasal and skin microbiomes along with other bacterial commensals and opportunistic pathogens. Nutrients are scarce in these habitats, demanding effective nutrient acquisition and competition strategies. How copes with phosphate limitation is still unknown. Wall teichoic acid (WTA), a polyol-phosphate polymer, could serve as a phosphate source, but whether can utilize it during phosphate starvation remains unknown. secretes a glycerophosphodiesterase, GlpQ, that cleaves a broad variety of glycerol-3-phosphate (GroP) headgroups of deacylated phospholipids, providing this bacterium with GroP as a carbon and phosphate source. Here we demonstrate that GlpQ can also use glycerophosphoglycerol derived from GroP WTA from coagulase-negative, , and, which share the nasal and skin habitats with Therefore, GlpQ is the first reported WTA-hydrolyzing enzyme, or teichoicase, from Activity assays revealed that unmodified WTA is the preferred GlpQ substrate, and the results from MS analysis suggested that GlpQ uses an exolytic cleavage mechanism. Importantly, GlpQ did not hydrolyze the ribitol-5-phosphate WTA polymers of, underscoring its role in interspecies competition rather than in cell wall homeostasis or WTA recycling. expression was strongly up-regulated under phosphate limitation, and GlpQ allowed to grow in the presence of GroP WTA as the sole phosphate source. Our study reveals a novel and unprecedented strategy of for acquiring phosphate from bacterial competitors under the phosphate-limiting conditions in the nasal or skin environments.

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supporting

confidence: 55%

Staphylococcus aureus counters phosphate limitation by scavenging wall teichoic acids from other staphylococci via the teichoicase GlpQ

Jorge

Schneider

Unsleber

et al. 2018

Journal of Biological Chemistry

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“…A GP‐PDE described in cell‐free extracts from the Gram‐positive Bacillus pumilus , DSM27, hydrolyzes phosphodiester bonds between adjacent glycerol units. The reported substrates are polyglycerophosphates, such as purified cell‐wall teichoic acid, as well as deacylated, unsubstituted lipoteichoic acid, di(glycerophospho)glycerol (deacylated cardiolipin) and mono(glycerophospho)glycerol .…”

Section: Other Bacterial Gp‐pdesmentioning

confidence: 99%

The emerging physiological roles of the glycerophosphodiesterase family

et al. 2014

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The glycerophosphodiester phosphodiesterases are evolutionarily conserved proteins that have been linked to several patho/physiological functions, comprising bacterial pathogenicity and mammalian cell proliferation or differentiation. The bacterial enzymes do not show preferential substrate selectivities among the glycerophosphodiesters, and they are mainly dedicated to glycerophosphodiester hydrolysis, producing glycerophosphate and alcohols as the building blocks that are required for bacterial biosynthetic pathways. In some cases, this enzymatic activity has been demonstrated to contribute to bacterial pathogenicity, such as with Hemophilus influenzae. Mammalian glyerophosphodiesterases have high substrate specificities, even if the number of potential physiological substrates is continuously increasing. Some of these mammalian enzymes have been directly linked to cell differentiation, such as GDE2, which triggers motor neuron differentiation, and GDE3, the enzymatic activity of which is necessary and sufficient to induce osteoblast differentiation. Instead, GDE5 has been shown to inhibit skeletal muscle development independent of its enzymatic activity.

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“…The observed decomposition and mineralization of TA is in contrast with the hypothesis that teichoic acids are resistant to decomposition (63). Mechanisms for the breakdown of wall teichoic acids under P limiting conditions are known (64,65), and it has also been observed that soil bacteria can produce specialized phosphodiesterases (glycerophosphodiesterases) to utilize exogenous teichoic acid as the sole P source (66), thereby releasing glycerol-3-phosphate (27,67,68). Interestingly, enzymes from the same highly conserved family (glycerophosphodiesterases) are known to be involved in the breakdown of both TA and PL (69).…”

Section: Decomposition Dynamics Of Teichoic Acids and Phospholipidsmentioning

confidence: 99%

Tracing 33P-labelled organic phosphorus compounds in two soils: New insights into decomposition dynamics and direct use by microbes

et al. 2023

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IntroductionOrganic phosphorus (Po) compounds constitute an important pool in soil P cycling, but their decomposition dynamics are poorly understood. Further, it has never been directly tested whether low molecular weight Po compounds are taken up by soil microbes in an intact form, which reduces the dependence of their P acquisition on extracellular phosphatases.MethodsWe investigated the short-term fate (24 h) of five 33P-labelled Po compounds (teichoic acids, phospholipids, DNA, RNA and soluble organophosphates) and 33P-labelled inorganic P (Pi) in two soils.ResultsWe found indications that soil microbial breakdown of phosphodiesters was limited by the depolymerization step, and that direct microbial uptake of Po occurred to a substantial extent.DiscussionWe postulate a trade-off between direct Po uptake and complete extracellular Po mineralization. These findings have profound consequences for our understanding of microbial P cycling in soils.

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A novel glycerophosphodiesterase from Bacillus pumilus

Cited by 10 publications

References 27 publications

Staphylococcus aureus counters phosphate limitation by scavenging wall teichoic acids from other staphylococci via the teichoicase GlpQ

Staphylococcus aureus counters phosphate limitation by scavenging wall teichoic acids from other staphylococci via the teichoicase GlpQ

The emerging physiological roles of the glycerophosphodiesterase family

Tracing 33P-labelled organic phosphorus compounds in two soils: New insights into decomposition dynamics and direct use by microbes

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