A novel gene, screened by cDNA-AFLP approach, contributes to lowering the acidity of fruit in apple

Yao, Yuxin; Li, Ming; Zhi, Liu; Hao, Yu‐Jin; Zhai, Heng

doi:10.1016/j.plaphy.2007.01.010

Cited by 52 publications

(33 citation statements)

References 27 publications

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“…Three milliliters of supernatant were passed through a 0.45 m membrane filter, and the filtrate was then analyzed using a capillary electrophoresis system (Beckman P/ACE, USA). The conditions for malate determination were previously described by Yao et al (2007), while the conditions for glucose, fructose and sucrose were as follows: uncoated capillary (50 m × 57 cm); applied voltage: 20 kV; pressure injection time: 5 s; indirect detection at = 214 nm; temp = 20 • C; 50 mM sodium benzoate + 1 mM CTAB; pH 12.…”

Section: Malate and Sugar Assaysmentioning

confidence: 99%

Isolation and characterization of an apple cytosolic malate dehydrogenase gene reveal its function in malate synthesis

Yao

Zhai

et al. 2011

Journal of Plant Physiology

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132

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Section: Malate and Sugar Assaysmentioning

confidence: 99%

Isolation and characterization of an apple cytosolic malate dehydrogenase gene reveal its function in malate synthesis

Yao

Zhai

et al. 2011

Journal of Plant Physiology

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132

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“…Apple total RNAs were extracted using a hot borate method described in our previous report (Yao et al 2007). Arabidopsis total RNAs were extracted using Trizol reagent (Invitrogen, USA).…”

Section: Real-time Quantitative Rt-pcr Analysis Of Gene Expressionmentioning

confidence: 99%

Molecular cloning and functional characterization of MdSOS2 reveals its involvement in salt tolerance in apple callus and Arabidopsis

Luo

et al. 2011

Plant Cell Rep

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Plants respond to various environmental stresses by activating "stress genes". CIPKs (CBL-interacting protein kinases) family genes play an important role in the process of stress response. In this study, a CIPK gene MdSOS2 was isolated from apple (Malus × Domestica). Sequence alignment and phylogenetic analysis showed that it is highly similar with Arabidopsis AtSOS2 and contained the conserved domains and motifs. Expression analysis demonstrated that MdSOS2 expressed in all tested organs at different levels, and positively in response to salt stress. Furthermore, the ectopic expression of MdSOS2 complemented the function of Arabidopsis sos2 mutant, and conferred enhanced salt tolerance to the transgenic Arabidopsis. Yeast two-hybrid assay indicated that the N-terminal of MdSOS2 protein physically interacted with MdSOS3 and AtSOS3, respectively, suggesting that SOS pathway operates in apple tree. Finally, MdSOS2 overexpression enhanced, while its suppression reduced the tolerance to salt in transgenic apple calluses, indicating that MdSOS2 acts as a positive regulator in response to salt stress in apple.

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“…However, a study on genes MdPEPC (EU315246), MdcyME (DQ280492) and MdVHA-A (EF128033) found that their expression and enzyme activities were different between low and high acid genotypes, suggesting that they may contribute to the variation of fruit acidity (Yao et al 2009). Another gene Mal-DDNA (DQ417661) of unknown function previously appeared to be associated with low acidity in apple fruit (Yao et al 2007). However, none of these genes are on chromosome 16 where the Ma gene resides.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of the Ma locus with pH and titratable acidity in apple

Wang

Brown

2011

Mol Breeding

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Apple fruit flavor is greatly affected by the level of malic acid, which is the major organic acid in mature apple fruit. To understand the genetic and molecular basis of apple fruit acidity, fruit juice pH and/or titratable acidity (TA) were measured in two half-sib populations GMAL 4595 [Royal Gala 9 PI (Plant Introduction) 613988] and GMAL 4590 (Royal Gala 9 PI 613971) of 438 trees in total. The maternal parent Royal Gala is a commercial variety and the paternal parents are two M. sieversii (the progenitor species of domestic apple) elite accessions. The lowacid trait segregates recessively and the overall acidity variations in the two populations were primarily controlled by the Ma (malic acid) locus, a major gene discovered in the 1950s (Nybom in Hereditas 45:332-350, 1959) and later mapped to linkage group 16 (Maliepaard et al. in Theor Appl Genet 97:60-73, 1998). The allele Ma has a strong additive effect in increasing fruit acidity and is incompletely dominant over ma. QTL (quantitative trait locus) analyses in GMAL 4595 mapped the major QTL Ma in both Royal Gala and PI 613988, the effects of which explained 17.0-42.3% of the variation in fruit pH and TA. In addition, two minor QTL, tentatively designated M2 and M3, were also detected for fruit acidity, with M2 on linkage group 6 of Royal Gala and M3 on linkage group 1 of PI 613988. By exploring the genome sequences of apple, eight new simple sequence repeat markers tightly linked to Ma were developed, leading to construction of a fine genetic map of the Ma locus that defines it to a physical region no larger than 150 kb in the Golden Delicious genome.

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A novel gene, screened by cDNA-AFLP approach, contributes to lowering the acidity of fruit in apple

Cited by 52 publications

References 27 publications

Isolation and characterization of an apple cytosolic malate dehydrogenase gene reveal its function in malate synthesis

Isolation and characterization of an apple cytosolic malate dehydrogenase gene reveal its function in malate synthesis

Molecular cloning and functional characterization of MdSOS2 reveals its involvement in salt tolerance in apple callus and Arabidopsis

Genetic characterization of the Ma locus with pH and titratable acidity in apple

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