Molecular cloning and functional characterization of MdSOS2 reveals its involvement in salt tolerance in apple callus and Arabidopsis

D, Hu; Li, Ming; Luo, Hua; Dong, Qinglong; Yao, Yuxin; You, Chun‐Xiang; Hao, Yu‐Jin

doi:10.1007/s00299-011-1189-5

Cited by 57 publications

(26 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Plant CIPK genes could be induced by different stresses, such as drought2344, salt45, and cold18. Various functional studies of plant CIPK genes provided clear evidence for their implication in stress responses.…”

Section: Discussionmentioning

confidence: 99%

Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean

Zhu

Chen

Liu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Calcium ion is an intracellular messenger that plays a central role in signal transduction pathways. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) signal network have shown different functions in the Ca2+ signaling process. In this work, we identified the entire soybean (Glycine max) CIPK gene family, which comprised 52 genes and divided into four subgroups (I to IV) based on phylogeny. The gene structural analysis separated these 52 genes into an intron-rich clade and an intron-poor clade. Chromosomal location analysis resulted in the identification of 22 duplicated blocks and six tandem duplication events. Phylogenetic classification of 193 CIPK proteins from representative plant species suggested that the intron-poor clade of CIPKs originated in seed plants. Analysis of global gene expression patterns of soybean CIPK family revealed that most intron-poor soybean CIPK genes are drought-inducible; a finding that was further confirmed using qRT-PCR. Our study provides a foundation for further functional analysis to reveal the roles that CIPKs and more specifically the intron-poor clade play in drought tolerance in soybean.

show abstract

Section: Discussionmentioning

confidence: 99%

Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean

Zhu

Chen

Liu

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Subsequent studies have demonstrated the functional conservation of the CBL-CIPK pathway in Populus (Yu et al, 2007), grape (Weinl and Kudla, 2009), sorghum (Li et al, 2010), and maize (Chen et al, 2011). Enhanced tolerance to diverse stresses has also been regularly observed in transgenic Arabidopsis because of the overexpression of CIPKs (Zhao et al, 2009;Chen et al, 2012), rice (Xiang et al, 2007), tomato Huertas et al, 2012), tobacco (Tripathi et al, 2009), and apple (Hu et al, 2012).…”

Section: Introductionmentioning

confidence: 97%

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

et al. 2014

View full text Add to dashboard Cite

ABSTRACT. CBL-interacting protein kinases (CIPKs) mediate many plant responses to abiotic stress. However, their functions are poorly understood in halophytes. In this study, we isolated a CIPK gene, NtCIPK2, from the halophyte Nitraria tangutorum. By sequence alignment and the construction of a phylogenetic tree, we found that NtCIPK2 is similar to CIPK2 proteins from other plants, and contains conserved domains and motifs. The promoter of NtCIPK2 harbors many cis-acting elements that might be recognized and bound by transcription factors that are related to hormones and stress responses. NtCIPK2 was ubiquitously and robustly expressed in all tested organs, and was induced by salinity, drought, heat, and cold stress. The overexpression of NtCIPK2 in Escherichia coli caused better growth against high salinity, alkalinity, and osmotic conditions, dehydration, and extreme temperatures (i.e., heat and cold) compared to the control. 4717©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 4716-4728 (2014) Cloning and characterization of the NtCIPK2 gene Thus, NtCIPK2 is a candidate gene that might improve the stress tolerance of crops and herbs through genetic manipulation.

show abstract

“…Additionally, the SOS pathway, in response to salinity stress, is also functionally conserved in plants (Martinez-Atienza et al, 2007; Tang et al, 2010). Moreover, some CIPK genes in Rosaceae species function similarly to AtCIPK24 / AtSOS2 in salt tolerance (Hu et al, 2012; Wang R.K. et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

Characterization of CIPK Family in Asian Pear (Pyrus bretschneideri Rehd) and Co-expression Analysis Related to Salt and Osmotic Stress Responses

Tang

Lin

et al. 2016

Front. Plant Sci.

View full text Add to dashboard Cite

Asian pear (Pyrus bretschneideri) is one of the most important fruit crops in the world, and its growth and productivity are frequently affected by abiotic stresses. Calcineurin B-like interacting protein kinases (CIPKs) as caladium-sensor protein kinases interact with Ca2+-binding CBLs to extensively mediate abiotic stress responses in plants. Although the pear genome sequence has been released, little information is available about the CIPK genes in pear, especially in response to salt and osmotic stresses. In this study, we systematically identified 28 CIPK family members from the sequenced pear genome and analyzed their organization, phylogeny, gene structure, protein motif, and synteny duplication divergences. Most duplicated PbCIPKs underwent purifying selection, and their evolutionary divergences accompanied with the pear whole genome duplication. We also investigated stress -responsive expression patterns and co-expression networks of CIPK family under salt and osmotic stresses, and the distribution of stress-related cis-regulatory elements in promoter regions. Our results suggest that most PbCIPKs could play important roles in the abiotic stress responses. Some PbCIPKs, such as PbCIPK22, -19, -18, -15, -8, and -6 can serve as core regulators in response to salt and osmotic stresses based on co-expression networks of PbCIPKs. Some sets of genes that were involved in response to salt did not overlap with those in response to osmotic responses, suggesting the sub-functionalization of CIPK genes in stress responses. This study revealed some candidate genes that play roles in early responses to salt and osmotic stress for further characterization of abiotic stress responses medicated by CIPKs in pear.

show abstract

Molecular cloning and functional characterization of MdSOS2 reveals its involvement in salt tolerance in apple callus and Arabidopsis

Cited by 57 publications

References 43 publications

Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean

Evolution of an intron-poor cluster of the CIPK gene family and expression in response to drought stress in soybean

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

Characterization of CIPK Family in Asian Pear (Pyrus bretschneideri Rehd) and Co-expression Analysis Related to Salt and Osmotic Stress Responses

Contact Info

Product

Resources

About