A novel fluorescence derivatization method combined with HPLC for determining the activities of endogenous caspase

Liu, Jiachi; Lu, Ye; Liang, Jianying

doi:10.1039/c2an35822k

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…With the development of analytical instruments, especially those of hyphenated techniques [e.g., high-performance liquid chromatography with ultraviolet detection (HPLC-UV), high-performance liquid chromatography with mass spectrometry (HPLC-MS) and gas chromatography with mass spectrometry (GC-MS)], the quantitation of compounds and their major metabolites can be produced conveniently. [1][2][3] Enhancing the detection limit, however, is still the key to obtaining information concerning trace components in the sample.…”

Section: Introductionmentioning

confidence: 99%

Improving the detection sensitivity of chromatography by stochastic resonance

Zhang

Guo

Xiang

et al. 2014

Analyst

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Improving the detection sensitivity of analytical instruments has been a challenging task for chemometricians since undetectability has been almost unavoidable in trace analysis, even under optimized experimental conditions and with the use of modern instruments. Various chemometrics methods have been developed which attempt to address this detection problem but with limited success (e.g., fast Fourier transform and wavelet transform). However, the application of stochastic resonance (SR) creates an entirely new and effective methodology. Stochastic resonance is a phenomenon which is manifested in non-linear systems where a weak signal can be amplified and optimized with the assistance of noise. In this review, we summarize the use of basic SR, optimization of parameters and its modifications, including periodic modulation stochastic resonance (PSRA), linear modulation stochastic resonance (LSRA), single-well potential stochastic resonance (SSR) and the Duffing oscillator algorithm (DOA) for amplifying sub-threshold small signals. We also review the advantages and the disadvantages of various SR procedures.

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Section: Introductionmentioning

confidence: 99%

Improving the detection sensitivity of chromatography by stochastic resonance

Zhang

Guo

Xiang

et al. 2014

Analyst

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“…An imbalance of caspase-3 levels in the apoptotic pathway may promote certain human diseases. Studies of experimental models have suggested that caspase-3 is a reliable indicator of apoptotic rate (13–16). However, data concerning the effects of EMF on apoptosis are limited (1).…”

Section: Introductionmentioning

confidence: 99%

Effect of Lycopersicon esculentum extract on apoptosis in the rat cerebellum, following prenatal and postnatal exposure to an electromagnetic field

Köktürk

Yardımoğlu

ÇELİKÖZLÜ

et al. 2013

Experimental and Therapeutic Medicine

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The expansion of mobile phone technology has raised concerns regarding the effect of 900-MHz electromagnetic field (EMF) exposure on the central nervous system. At present, the developing human brain is regularly exposed to mobile telephones, pre- and postnatally. Several studies have demonstrated the acute effects of EMF exposure during pre- or postnatal periods; however, the chronic effects of EMF exposure are less understood. Thus, the aim of the present study was to determine the chronic effects of EMF on the pre- and postnatal rat cerebellum. The control group was maintained in the same conditions as the experimental groups, without the exposure to EMF. In the EMF1 group, the rats were exposed to EMF during pre- and postnatal periods (until postnatal day 80). In the EMF2 group, the rats were also exposed to EMF pre- and postnatally; in addition, however, they were provided with a daily oral supplementation of Lycopersicon esculentum extract (∼2 g/kg). The number of caspase-3-labeled Purkinje neurons and granule cells present in the rats in the control and experimental groups were then counted. The neurodegenerative changes were studied using cresyl violet staining, and these changes were evaluated. In comparison with the control animals, the EMF1 group demonstrated a significant increase in the number of caspase-3-labeled Purkinje neurons and granule cells present in the cerebellum (P<0.001). However, in comparison with the EMF1 group, the EMF2 group exhibited significantly fewer caspase-3-labeled Purkinje neurons and granule cells in the cerebellum. In the EMF1 group, the Purkinje neurons were revealed to have undergone dark neuron degenerative changes. However, the presence of dark Purkinje neurons was reduced in the EMF2 group, compared with the EMF1 group. The results indicated that apoptosis and neurodegeneration in rats exposed to EMF during pre- and postnatal periods may be reduced with Lycopersicon esculentum extract therapy.

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“…The active form of caspase-3 can specifically recognize the tetra-peptide motif of Asp-Glu-Val-Asp(DEVD) and hydrolyze the peptide bonds at the C-terminal after the aspartic acid residues. 5 Thereupon, various analytical methods have been developed for the detection and quantification of the caspase-3 activity based on the sequential cleavage activity of the protease employing different techniques, including fluorescence, [6][7][8][9] electrochemistry, 10 chemiluminescence, 11 colorimetry, 12,13 flow cytometry, 14 mass spectroscopy, 15 and surface plasmon resonance. 16 These methods show advantages such as high sensitivity, good biocompatibility for in vivo applications, and good specificity.…”

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confidence: 99%