EPR-based<i>in situ</i>enzymatic activity detection of endogenous caspase-3 in apoptosis cell lysates

Liu, Aokun; Kuang, Jian; Zhou, Yemian; Xu, An; Tian, Changlin; Yu, Lu

doi:10.1039/d2cc02361j

Cited by 1 publication

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References 26 publications

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“…Caspase‐3 plays an essential role in the apoptosis process of cancer cells, and activated caspase‐3 is generally recognized as a signature of apoptosis. [ 44 ] Excessive amounts of ROS and the decline of MMP usually release proapoptotic factors from mitochondria into cytosol and sequentially activate the caspase cascade. [ 45,46 ] Therefore, we investigated apoptosis and caspase‐3 activities by GreenNuc™/Annexin V‐mCherry double staining.…”

Section: Resultsmentioning

confidence: 99%

Syntheses, structures, and the in vitro anticancer mechanism of triorganotin (IV) complexes based on 4,4′‐stilbenedicarboxylic acid

et al. 2023

Applied Organom Chemis

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Five triorganotin (IV) complexes, [(Ph3Sn)2L] (1) [(Me3Sn)2L]n (2), [(n‐Bu3Sn)2L]n (3), [(n‐Bu 3Sn)2L(4,4′‐bpy)]n (4), [(n‐Bu3Sn)2L(bpe)]n (5) [H2L = 4,4′‐stilbenedicarboxylic acid, 4,4′‐bpy = 4,4′‐bipyridine, bpe = 4,4′‐vinylenedipyridine], were successfully prepared and structurally characterized by FT‐IR, elemental analysis, NMR spectroscopy, PXRD, and X‐ray crystallography. Complex 1 is a monomer containing two tin atoms, and 1D infinite chains can be formed between the monomers through CH···π interactions. Complexes 2 and 3 represent 2D network structures containing tetranuclear 38‐membered rings. Meanwhile, complexes 4 and 5 are 1D chain‐like structures and form the 2D network and 3D stereo structure via CH···O intermolecular interactions, respectively. The anticancer activities of the complexes were investigated against different cancer cells (A549, HeLa, and HepG‐2). Complexes 1 and 3–5 showed superior anticancer activity. Meanwhile, the anticancer mechanism of complex 1 was studied with the results that complex 1 can promote the production of excessive reactive oxygen species, induce mitochondrial membrane permeability depolarization, and release proapoptotic factors from mitochondria into the cytosol, followed by caspase‐3 activation, nuclei damage, and apoptosis. Excitingly, complex 1 also has good antimetastatic ability toward HepG‐2 cancer cells.

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Section: Resultsmentioning

confidence: 99%

Syntheses, structures, and the in vitro anticancer mechanism of triorganotin (IV) complexes based on 4,4′‐stilbenedicarboxylic acid

et al. 2023

Applied Organom Chemis

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EPR-basedin situenzymatic activity detection of endogenous caspase-3 in apoptosis cell lysates

Abstract: Caspase-3 plays vital role in cell apoptosis and related diseases. Detection and characterization of endogenous active caspase-3 are of immense value not only for mechanism studies of apoptosis but also...

Cited by 1 publication

References 26 publications

Syntheses, structures, and the in vitro anticancer mechanism of triorganotin (IV) complexes based on 4,4′‐stilbenedicarboxylic acid

Syntheses, structures, and the in vitro anticancer mechanism of triorganotin (IV) complexes based on 4,4′‐stilbenedicarboxylic acid

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