A novel feedback catalytic hairpin assembly strategy for sensitive mRNA imaging in living cells

Liang, Pengying; Sun, Mengxu; Chen, Mengyu; Li, Tong; Huang, Ting; Zhou, Qingying; Li, Chunrong; Xie, Baoping; Chen, Jinxiang; Dai, Zong; Tian, Yuanxin; Chen, Jun

doi:10.1016/j.snb.2023.135032

Sensors and Actuators B: Chemical

2024

DOI: 10.1016/j.snb.2023.135032

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A novel feedback catalytic hairpin assembly strategy for sensitive mRNA imaging in living cells

Pengying Liang,

Mengxu Sun,

Mengyu Chen

et al.

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2024

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“…Recently, β-hydroxyl iron oxide (β-FeOOH) nanomaterials have been widely used as a transfection material in the fields of drug delivery, disease therapy, and cell imaging. − β-FeOOH nanomaterials have good biocompatibility, low toxicity, and stability, and its transfection efficiency and selectivity can be regulated by modifying its surface properties and structures. − Therefore, β-FeOOH can be considered a promising nucleic acid transfection material for achieving efficient nucleic acid delivery.…”

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confidence: 99%

Toehold Strand Displacement-Mediated Exponential HCR for Highly Sensitive and Specific Analysis of miRNA in Living Cells

Sun,

Zhou,

Peng

et al. 2024

Anal. Chem.

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As an important disease biomarker, the development of sensitive detection strategies for miRNA, especially intracellular miRNA imaging strategies, is helpful for early diagnosis of diseases, pathological research, and drug development. Hybridization chain reaction (HCR) is widely used for miRNA imaging analysis because of its high specificity and lack of biological enzymes. However, the classic HCR reaction exhibits linear amplification with low efficiency, limiting its use for the rapid analysis of trace miRNA in living cells. To address this problem, we proposed a toehold-mediated exponential HCR (TEHCR) to achieve highly sensitive and efficient imaging of miRNA in living cells using β-FeOOH nanoparticles as transfection vectors. The detection limit of TEHCR was as low as 92.7 fM, which was 8.8 × 103 times lower compared to traditional HCR, and it can effectively distinguish single-base mismatch with high specificity. The TEHCR can also effectively distinguish the different expression levels of miRNA in cancer cells and normal cells. Furthermore, TEHCR can be used to construct OR logic gates for dual miRNA analysis without the need for additional probes, demonstrating high flexibility. This method is expected to play an important role in clinical miRNA-related disease diagnosis and drug development as well as to promote the development of logic gates.

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confidence: 99%

Toehold Strand Displacement-Mediated Exponential HCR for Highly Sensitive and Specific Analysis of miRNA in Living Cells

Sun,

Zhou,

Peng

et al. 2024

Anal. Chem.

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A novel feedback catalytic hairpin assembly strategy for sensitive mRNA imaging in living cells

Cited by 1 publication

References 35 publications

Toehold Strand Displacement-Mediated Exponential HCR for Highly Sensitive and Specific Analysis of miRNA in Living Cells

Toehold Strand Displacement-Mediated Exponential HCR for Highly Sensitive and Specific Analysis of miRNA in Living Cells

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