A novel experimental approach to investigate the effect of different agitation methods using sodium hypochlorite as an irrigant on the rate of bacterial biofilm removal from the wall of a simulated root canal model

Mohmmed, Saif alarab; Vianna, Morgana Eli; Penny, Matthew R.; Hilton, Stephen T.; Mordan, Nicola; Knowles, Jonathan C.

doi:10.1016/j.dental.2016.07.013

Cited by 32 publications

(36 citation statements)

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“…The construction of the simulated root canal was performed according to Mohmmed et al . (,b) with the modification of a more complex root canal anatomy, that is the presence of a lateral canal. The preparation of microbial strains, generation of biofilms, irrigation methods, data collection and analysis were based on Mohmmed et al .…”

Section: Methodsmentioning

confidence: 99%

“…The preparation of microbial strains, generation of biofilms, irrigation methods, data collection and analysis were based on Mohmmed et al . (,b) and described in detail as follows.…”

Section: Methodsmentioning

confidence: 99%

“…The lower view shows the printed two halves, and when they are reassembled, a straight simple canal of 18 mm length, apical size 30 and a .06 taper is created with the lateral canal of 3 mm length, 0.3 mm diameter adapted from Mohmmed et al . (,b) with permission.…”

Section: Methodsmentioning

confidence: 99%

“…, Mohmmed et al . ,b) or by increasing its concentration (Mohmmed et al . ), these studies focused on irrigation within simple straight root canals.…”

Section: Introductionmentioning

confidence: 99%

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Investigations into in situ Enterococcus faecalis biofilm removal by passive and active sodium hypochlorite irrigation delivered into the lateral canal of a simulated root canal model

et al. 2017

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Section: Methodsmentioning

confidence: 99%

“…The preparation of microbial strains, generation of biofilms, irrigation methods, data collection and analysis were based on Mohmmed et al . (,b) and described in detail as follows.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…, Mohmmed et al . ,b) or by increasing its concentration (Mohmmed et al . ), these studies focused on irrigation within simple straight root canals.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Investigations into in situ Enterococcus faecalis biofilm removal by passive and active sodium hypochlorite irrigation delivered into the lateral canal of a simulated root canal model

et al. 2017

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“…Advantages of in vitro biofilm models were described in our previous study (20). The model is acrylate base polymers consisting of a mixture of methacrylic acid and a photo-initiator, which is designed to work with 3D Printer to produce solid plastic parts on curing.…”

Section: Discussionmentioning

confidence: 99%

The effect of sodium hypochlorite concentration and irrigation needle extension on biofilm removal from a simulated root canal model

et al. 2017

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To investigate the effect of sodium hypochlorite concentration and needle extension on removal of Enterococcus faecalis biofilm, sixty root canal models were 3D printed.Biofilms were grown on the apical 3 mm of the canal for 10 days. Irrigation for 60s with 9 mL of either 5.25% or 2.5% NaOCl or water was performed using a needle inserted either 3 or 2mm from the canal terminus and imaged using fluorescence microscopy and residual biofilm percentages were calculated using imaging software. The data were analysed using analysis of covariance and two-sample ttests. A significance level of 0.05 was used throughout. Residual biofilm was less using 5.25% than with 2.5% NaOCl. Statistically significant biofilm removal was evident with the needle placed closer to the canal terminus. A greater reduction of available chlorine and pH was noted as the concentration increased. One-minute irrigation was not sufficient for complete biofilm removal.

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Irrigating Solutions, Devices, and Techniques

Boutsioukis

Arias-Moliz

2021

Endodontic Materials in Clinical Practice

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A novel experimental approach to investigate the effect of different agitation methods using sodium hypochlorite as an irrigant on the rate of bacterial biofilm removal from the wall of a simulated root canal model

Abstract: The 3D printing method provided a novel model to create a root canal simulation for studying and understanding a real-time biofilm removal under microscopy. Ultrasonic agitation of NaOCl left the least amount of residual biofilm in comparison to sonic and gutta-percha agitation methods.

Cited by 32 publications

References 57 publications

Investigations into in situ Enterococcus faecalis biofilm removal by passive and active sodium hypochlorite irrigation delivered into the lateral canal of a simulated root canal model

Investigations into in situ Enterococcus faecalis biofilm removal by passive and active sodium hypochlorite irrigation delivered into the lateral canal of a simulated root canal model

The effect of sodium hypochlorite concentration and irrigation needle extension on biofilm removal from a simulated root canal model

Irrigating Solutions, Devices, and Techniques

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