A novel electrospun polyurethane nanofibre membrane – production parameters and suitability for wastewater (WW) treatment

Lev, Jaroslav; Holba, Marek; Došek, Michal; Kalhotka, Libor; Mikula, Přemysl; Kimmer, Dušan

doi:10.2166/wst.2014.034

Cited by 11 publications

(8 citation statements)

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“…Immobilized enzyme performance, to a great extend depends on the structure of support. Studies reveal that nano structured supports were found to preserve catalytic activity and ensure efficiency of immobilized enzyme to a great level (Lev et al 2014;Maryskova et al 2019). Likewise, nanofibrous support provides greater porosity and good interconnectivity there by acting as one of the ideal systems for immobilization.…”

Section: Introductionmentioning

confidence: 99%

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Paul²,

Sunkar

2021

Water Science and Technology

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Azo dyes are widely used in textile industries. A significant portion of these recalcitrant dyes are being discharged to the natural waters. Due to their low biodegradability they pose serious pollution problems if untreated. In this work, decolourization studies of Acid Red 1 (AR1) by laccase enzyme immobilized onto Zein – Polyvinyl pyrrolidone composite nanofiber is done. The nanofibers were characterized by SEM and FTIR analysis. pH and temperature profiles of immobilized enzyme were found to be broader than its free counterpart. The Km value was found to be 0.243 mM for free laccase and 0.958 mM for immobilized laccase. Similarly Vmax for the free enzyme was 3.572 U/mg compared to 2.48 U/mg of immobilized laccase. The relative activity of immobilized laccase was 64.91% after storage for 30 days at room temperature while it was 28.64% for free laccase. The temperature and pH for AR 1 decolorization were optimized and was found to be 60 °C and 5 respectively. Also decolorization percentage was found to be 91.67% for immobilized laccase and 72.03% of free laccase in the presence of natural mediators like vanillin. From phytotoxicity studies it was found that the germination rate, shoot and root length was increased compared to untreated dye. Therefore, Zein-PVP nanofiber immobilized laccase could be an ideal candidate for the textile dye decolorization.

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Section: Introductionmentioning

confidence: 99%

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Paul²,

Sunkar

2021

Water Science and Technology

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“…Membrane filtration has recently become a good alternative to more commonly used water purification technologies based on chlorination or oxidation processes, mainly due to their reducing costs and high microbial removal efficacy. Besides other commercially available filtration membranes, electrospun nanofiber membranes have great potential, since they represent even more promising choices for high flux microfiltration applications (Kaur et al 2014; Lev et al 2014; Seyed Shahabadi et al 2016; Wang et al 2012). Moreover, either polymer matrix for nanofiber preparation or surface of the pristine membrane itself can be functionalized with various techniques or antimicrobial agents that suppress biofilm formation, i.e.…”

Section: Introductionmentioning

confidence: 99%

Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes

Trávníčková

Mikula

Opršal³

et al. 2019

AMB Expr

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We developed a simple and fast microplate assay for evaluation of the antimicrobial activity of electrospun nanofiber filtration membranes or similar porous materials for water treatment technologies. Resazurin (alamarBlue®) was used as an indicator of the amount of viable experimental microorganisms Gram-negative Escherichia coli, Gram-positive Enterococcus faecalis, and natural wastewater treatment plant effluent bacteria. A bacterial inoculum of concentration 1–3 × 105 CFU mL−1 was pipetted onto the surface of assessed both functionalized and respective control membranes and incubated in 12-well plates for 4 h at 37 °C. Kinetics of resazurin metabolization, i.e. its reduction to fluorescent resorufin, was evaluated fluorimetrically (λex520/λem590 nm). A number of viable bacteria on the membranes expressed as CFU mL−1 was calculated from the kinetic curves by using calibration curves that were constructed for both experimental bacterial species. Antimicrobial activities of the membranes were evaluated by either resazurin assay or modified ISO 20743 plate count assay. Results of both assays showed the significant antimicrobial activity of membranes functionalized with silver nanoparticles for both bacterial species and wastewater treatment plant effluent bacteria as well (log CFU reduction compared to control membrane > 4), while membranes containing specific quaternary ammonium salts were inefficient (log CFU reduction < 1). The suitability of resazurin microplate assay for testing nanofiber filtration membranes and analogous matrices has proven to be a faster and less demanding alternative to the traditionally used approach providing comparable results.

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“…Lev, J. et al also reported using electrospinning technique to prepare PU-NFs [51]. The as-prepared nanofibers were collected on polypropylene textiles, as a range of flat sheet non-woven nanofiber membrane layers.…”

Section: Nanosynthesis Of Representative Polymers Used In Construcmentioning

confidence: 99%

Synthesis, Morphologies and Building Applications of Nanostructured Polymers

Shah

2017

Polymers

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Nanostructured polymers (NSPs) are polymeric materials in the size of nanoscale, normally consisting of nanoparticles, nanofibers, nanowires, nanospheres and other morphologies. Polymer nanoparticles (PNPs) can be fabricated either by physical methods (i.e., solvent evaporation, nanoprecipitation, salting out) or by direct nanosynthesis, using micro-or nanoemulsions with nanoreactor compartments to perform polymerization. Polymer nanofibers (PNFs) can be produced via various techniques and the most commonly used approach is electrospinning, whereby a charged solution of a polymer when exposed to an opposite high electric field is pulled into long thin nanofibers. NSPs in general exhibit enhanced properties such as excellent structural and mechanical properties, making them promising candidates for some particular building applications. A variety of PNFs have been developed and used for noise and air pollution filtration. Moreover, PNFs can also be fabricated with phase change materials which are usually employed for thermal energy storage in construction industry. In this review, we will summarize the morphologies and nanosynthesis methods of NSPs, in particular, PNPs and PNFs. In addition, representative NSPs mainly used in construction are introduced for building applications.

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A novel electrospun polyurethane nanofibre membrane – production parameters and suitability for wastewater (WW) treatment

Cited by 11 publications

References 0 publications

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes

Synthesis, Morphologies and Building Applications of Nanostructured Polymers

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