A novel electron beam-based method for the immobilization of trypsin on poly(ethersulfone) and poly(vinylidene fluoride) membranes

Starke, S.; Went, Marco; Prager, Andrea; Schulze, Agnes

doi:10.1016/j.reactfunctpolym.2013.02.013

Cited by 24 publications

(23 citation statements)

References 15 publications

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“…However, the changes are not significant, and we can assume that the membrane structure was not destroyed or altered by the EB treatment nor blocked by the immobilized enzyme. Further investigation on the impact of EB on the membrane material was published before and confirms these results [22,24,25]. Table 3.…”

Section: Resultssupporting

confidence: 76%

“…The EB treatment results in the generation of a mixture of ions, excited molecules and free radicals as described for the radiolysis of water [45] ensuring the activation of both the dissolved trypsin [25,26,46] as well as of the membranes [22][23][24][27][28][29]47]. The formed radicals/activated species can undergo various reactions, such as cross-linking or recombination reactions.…”

Section: Resultsmentioning

confidence: 99%

“…This technology enables direct immobilization of functional organic molecules [22,23], engineering polymers [24], or even biomolecules [25,26] on a membrane's surface. The use of low-energy EB radiation combines surface activation (formation of activated species) of the membrane polymer and simultaneous covalent immobilization of the desired compounds from an aqueous system.…”

Section: Polymers 2015 7mentioning

confidence: 99%

“…The enzyme was immobilized via EB radiation on different PVDF microfiltration membranes according to a recently described method [25]. The modifications were performed by immersing the membranes for 5 min into an aqueous buffer solution (HEPES pH 8.0) of trypsin (2 mg/mL) at room temperature followed by electron beam irradiation with an irradiation dose of 150 kGy.…”

Section: Preparation and Characterization Of Biocatalytic Membranesmentioning

confidence: 99%

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Biocatalytic Self-Cleaning Polymer Membranes

et al. 2015

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Polymer membrane surfaces have been equipped with the digestive enzyme trypsin. Enzyme immobilization was performed by electron beam irradiation in aqueous media within a one-step method. Using this method, trypsin was covalently and side-unspecific attached to the membrane surface. Thus, the use of preceding polymer functionalization and the use of toxic solvents or reagents can be avoided. The resulting membranes showed significantly improved antifouling properties as demonstrated by repeated filtration of protein solutions. Furthermore, the biocatalytic membrane can be simply "switched on" to actively degrade a fouling layer on the membrane surface and regain the initial permeability. The membrane pore structure (pore size and porosity) was neither damaged by the electron beam treatment nor blocked by the enzyme loading, ensuring a stable membrane performance.

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Section: Resultssupporting

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Polymers 2015 7mentioning

confidence: 99%

Section: Preparation and Characterization Of Biocatalytic Membranesmentioning

confidence: 99%

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Biocatalytic Self-Cleaning Polymer Membranes

et al. 2015

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“…Considering the q m of the PVDF/PES-g-PAA-PrA membranes for pore size 0.45 µm shown in Table 3, the ratio of active Protein A modified on the PVDF-g-PAA-PrA membrane would be higher than that of the PES-g-PAAPrA membrane. As a recent related to the study of fabricating functional polymers, Starke et al [32] have introduced a technique for covalent immobilization of trypsin on hydrophilic PVDF and PES membranes both with 0.22 µm pores using electron beam irradiation (150 kGy). Comparing the trypsin concentration ).…”

Section: Determination Of the Human Igg Adsorption Capacity Of Affinimentioning

confidence: 99%

Preparation and characterization of Protein A-immobilized PVDF and PES membranes

Akashi¹,

Kuroda²

2015

Express Polym. Lett.

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Functional hydrogels with a multicatalytic activity for bioremediation: Single‐step preparation and characterization

Spizzirri

Curcio

Cirillo

et al. 2016

J of Applied Polymer Sci

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In a single‐step free radical reaction, multicatalytic hydrogels were synthesized by covalent immobilization of Pancreatin onto a film composed of Acrylamide and Polyethylene glycol dimethacrylate750. Hydrogels were characterized by determination of their dynamic swelling ratios and each catalytic activity was extensively investigated by determination of kinetic parameters KM and Vmax. The immobilization process was found to preserve the hydrolytic properties of Pancreatin (Protease, Lipase, and Amylase catalytic activities). Catalytic efficiencies were the highest with Protease and the lowest with Amylase. Reusability values higher than 60% after 10 repeated cycles proved the applicability of the proposed material in industrial practice. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43338.

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A novel electron beam-based method for the immobilization of trypsin on poly(ethersulfone) and poly(vinylidene fluoride) membranes

Cited by 24 publications

References 15 publications

Biocatalytic Self-Cleaning Polymer Membranes

Biocatalytic Self-Cleaning Polymer Membranes

Preparation and characterization of Protein A-immobilized PVDF and PES membranes

Functional hydrogels with a multicatalytic activity for bioremediation: Single‐step preparation and characterization

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