A novel electrochemiluminescent detection of protein biomarker using l-cysteine and in situ generating coreactant for signal amplification

Sun, Ailing; Qi, Qingan; Wang, Xuannian; Bie, Ping

doi:10.1016/j.snb.2013.11.007

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…One molecular PTCA possessed four -COOH, thus it could combine more Cys compared with the MWCNTs without PTCA, and the chiral recognition ability was enhanced obviously. 15,18,19 According to the above results, the MWCNTs and PTCA could not introduce the chiral recognition ability for the D-and L-DOPA exhibited the same voltammetry curves on the MWCNTs-PTCA/GCE. After the MWCNTs-PTCA were assembled with the Cys further, the D-and L-DOPA exhibited different voltammetry curves on the MWCNTs-PTCA-Cys modified electrodes.…”

Section: Resultsmentioning

confidence: 80%

“…In order to obtain better dispersity and introduce more binding sites, a series of methods have been adopted for the surface functionalization of MWCNTs, such as aggressive oxidation treatment with HNO 3 or a HNO 3 -H 2 SO 4 mixture, 12,13 wrapping with polymer, 14,15 grafting of ionic liquid, 16,17 modification by π-stacking, 18 etc. 3,4,9,10-perylene tetracarboxylic acid (PTCA), as a bifunctional molecule with an aromatic pyrenyl group and four carboxyl groups, has been used to functionalize MWCNTs via π-π stacking, [18][19][20] which could obtain highly dispersed carboxylic multiwalled carbon nanotubes. This strategy not only introduces large amounts of carboxyl groups (-COOH) to the surface of MWCNTs, but also overcomes the structural damage and the loss of electronic conductivity of MWCNTs caused by harsh acid oxidation.…”

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confidence: 99%

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Study on Chiral Nanocomposite for Recognition of DOPA Enantiomers via Square Wave Voltammetry

Ran

Xuan

et al. 2015

J. Electrochem. Soc.

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An electrochemical sensor was explored for recognizing 3,4-dihydroxyphenylalanine (DOPA) enantiomers based on L-cysteine covalently binding with the multi-walled carbon nanotubes functionalized with 3,4,9,10-perylene tetracarboxylic acid (MWCNTs-PTCA-Cys). Here two different ways of square wave voltammetry (SWV) and cyclic voltammetry (CV) were utilized to investigate the stereospecific interaction between the MWCNTs-PTCA-Cys nanocomposite and DOPA enantiomers. The D-DOPA and L-DOPA exhibited different response in both CV and SWV, in addition, the difference in SWV was more obvious and reached to 264.9 μA, suggesting that the enantiomers could be successfully distinguished by MWCNTs-PTCA-Cys nanocomposite, and the selection of electrochemical technique played an important role in enantioselective recognition. Under the optimum conditions, the detection limits of D-DOPA and L-DOPA were 2.5 μM and 4.6 μM (S/N = 3), respectively, with a linear range of 1.0 × 10−5 M to 5.0 × 10−3 M. This work provided a simple method to recognize and determine DOPA enantiomers with rapid response, excellent recognition ability, high sensitivity and good stability.

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Section: Resultsmentioning

confidence: 80%

mentioning

confidence: 99%

Study on Chiral Nanocomposite for Recognition of DOPA Enantiomers via Square Wave Voltammetry

Ran

Xuan

et al. 2015

J. Electrochem. Soc.

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“…Therefore, it is necessary to develop a technique for cysteine detection. L‐cysteine detection has been reported using fluorimetry , chemiluminescence , high‐performance liquid chromatography , and electrochemistry . Among these, electrochemical detection has received considerable attention owing to the high sensitivity, speed, and low cost .…”

Section: Introductionmentioning

confidence: 99%

Photoelectrochemical Detection of L‐Cysteine with a Covalently Grafted ZnTAPc‐Gr‐based Probe

et al. 2020

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L‐cysteine plays a vital role in organisms, and is an important biomarker for many pathological diseases that seriously affect human health. In the study, a photoelectrochemical (PEC) probe with zinc‐tetramine phthalocyanine covalently grafted to graphene oxide (ZnTAPc‐Gr) was developed for L‐cysteine detection. Graphene oxide (GO) with carboxyl was used to immobilize zinc‐tetramine phthalocyanine (ZnTAPc) with amidogen (a graft structure formed by an amide covalent bond), which could firmly immobilize ZnTAPc, thereby improving the photoelectrochemical performance and stability. L‐cysteine molecule, an electron acceptor, was specifically recognized by the PEC probe, exhibiting a decrease in the photocurrent signal. Under the optimal experimental conditions, the fabricated PEC probe exhibited excellent performance in L‐cysteine analysis within a linear range of 0.25–113 μM and a detection limit of 11.4 nM. The PEC probe showed high sensitivity, selectivity, and stability. The method described herein provides an effective strategy for PEC probe construction for L‐cysteine detection, and can also serve as a promising PEC platform for the analyses of other small molecules.

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Analytical applications of nanomaterials in electrogenerated chemiluminescence

2014

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This critical review covers the use of carbon nanomaterials (single-wall carbon nanotubes, multi-wall carbon nanotubes, graphene, and carbon quantum dots), semiconductor quantum dots, and composite materials based on the combination of the aforementioned materials, for analytical applications using electrogenerated chemiluminescence. The recent discovery of graphene and related materials, with their optical and electrochemical properties, has made possible new uses of such materials in electrogenerated chemiluminescence for biomedical diagnostic applications. In electrogenerated chemiluminescence, also known as electrochemiluminescence (ECL), electrochemically generated intermediates undergo highly exergonic reactions, producing electronically excited states that emit light. These electron-transfer reactions are sufficiently exergonic to enable the excited states of luminophores, including metal complexes, quantum dots and carbon nanocrystals, to be generated without photoexcitation. In particular, this review focuses on some of the most advanced and recent developments (especially during the last five years, 2010-2014) related to the use of these novel materials and their composites, with particular emphasis on their use in medical diagnostics as ECL immunosensors.

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A novel electrochemiluminescent detection of protein biomarker using l-cysteine and in situ generating coreactant for signal amplification

Cited by 8 publications

References 28 publications

Study on Chiral Nanocomposite for Recognition of DOPA Enantiomers via Square Wave Voltammetry

Study on Chiral Nanocomposite for Recognition of DOPA Enantiomers via Square Wave Voltammetry

Photoelectrochemical Detection of L‐Cysteine with a Covalently Grafted ZnTAPc‐Gr‐based Probe

Analytical applications of nanomaterials in electrogenerated chemiluminescence

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