A novel Ehrlichia genotype detected in naturally infected cattle in North America

Gajadhar, Alvin A.; Lobanov, Vladislav A.; Scandrett, W. Brad; Campbell, John B.; Al-Adhami, Batol

doi:10.1016/j.vetpar.2010.06.034

Cited by 38 publications

(60 citation statements)

References 18 publications

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“…BOV2010. Diagnosis of infection in 7 animals from this group was described in a previous study, 8 whereas infection of the other 2 animals was confirmed in the present study following the PCR protocol described previously. 8 Group 2.…”

Section: Samplessupporting

confidence: 56%

“…Diagnosis of infection in 7 animals from this group was described in a previous study, 8 whereas infection of the other 2 animals was confirmed in the present study following the PCR protocol described previously. 8 Group 2. Thirteen serum samples (1-6 per animal) were collected at different periods from 3 field cattle from Manitoba naturally infected with A. marginale (2009) and stored at -20°C.…”

Section: Samplessupporting

confidence: 56%

“…BOV2010, remained seronegative by both tests, presumably because animals E and G were euthanized at 28 and 33 DPI, respectively and had not yet generated a detectable immune response. Molecular analysis of blood from experimentally infected cattle confirmed infection with Ehrlichia in all animals except 1 animal (F) 8 euthanized at 28 DPI that also remained seronegative by both cELISA and IFAT.…”

Section: Discussionmentioning

confidence: 88%

“…Five calves were inoculated with single or pooled aliquots of citrated whole blood collected from 7 of the naturally infected British Columbia cattle included in group 1 as described previously. 8 Two positive control calves were inoculated with stabilates of A. marginale from cattle (Manitoba) or bison (Saskatchewan). One calf remained uninfected as a negative control.…”

Section: Samplesmentioning

confidence: 99%

“…Based on this, a 1:160 antibody titer was established as the cut-off value using antigen smears from either source. 8 or current study (2 animals). For group 2, infection was confirmed by PCR in an earlier study.…”

Section: Serological Testsmentioning

confidence: 99%

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Serological cross-reactivity between Anaplasma marginale and an Ehrlichia species in naturally and experimentally infected cattle

et al. 2011

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Seroconversion and cross-reactivity in cattle infected with Anaplasma marginale or a recently described Ehrlichia species (BOV2010 from British Columbia, Canada) were investigated. The study used 76 samples from 20 animals, a commercially available competitive enzyme-linked immunosorbent assay (cELISA) for bovine anaplasmosis, and an indirect fluorescent antibody test (IFAT). Blood smear examination and/or polymerase chain reaction assay were performed to confirm or rule out the presence of Anaplasma or Ehrlichia. Samples comprised 3 groups. Group 1 consisted of 24 samples from 9 cattle naturally infected with Ehrlichia sp. BOV2010. Group 2 had 13 samples from 3 A. marginale–infected cattle from Manitoba, Canada. Group 3 had 39 samples, consisting of 26 from 5 calves experimentally infected with Ehrlichia sp. BOV2010, 10 from 2 calves experimentally infected with A. marginale from cattle (Manitoba) or bison (Saskatchewan), and 3 from an uninfected calf. All samples from cattle naturally or experimentally infected with Ehrlichia sp. BOV2010 or A. marginale were seropositive for A. marginale by both cELISA and IFAT, except 3 calves euthanized at 28 and 33 days post-inoculation (DPI) that did not seroconvert. Antibodies were detected in 2 experimental animals inoculated with Ehrlichia sp. BOV2010, as early as 28 and 33 DPI by the cELISA and IFAT, respectively, and by 42 DPI for both tests. The current study demonstrates that the specificity of the recombinant major surface protein 5 (MSP5) antigen is not restricted to Anaplasma spp., which reduces the utility of the test for serological diagnosis of bovine anaplasmosis in regions where Ehrlichia sp. BOV2010–infected cattle might exist.

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Section: Samplessupporting

confidence: 56%

Section: Samplessupporting

confidence: 56%

Section: Discussionmentioning

confidence: 88%

Section: Samplesmentioning

confidence: 99%

Section: Serological Testsmentioning

confidence: 99%

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Serological cross-reactivity between Anaplasma marginale and an Ehrlichia species in naturally and experimentally infected cattle

et al. 2011

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First detection of Ehrlichia minasensis in Hyalomma marginatum ticks collected from cattle in Corsica, France

Cicculli

Masse

Capai

et al. 2019

Veterinary Medicine & Sci

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Ehrlichiosis are severe, feverish tick‐borne illnesses caused by specific species within the genus Ehrlichia (Anaplasmataceae family). Recent data suggest that ruminants in Corsica area reservoir for several Anaplasmataceae species. The purpose of our study was to determine whether Ehrlichia species could be detected in ticks collected in Corsican ruminants by using molecular methods. Ticks were collected in northern Corsica: (i) in May 2016 from sheep bred in one farm located in a 5000‐inhabitants village and (ii) from cattle in June and July 2016 in a slaughterhouse. There sheep and cattle whole skin was inspected and ticks were collected manually. A total of 647 ticks was collected in northern Corsica during this study: 556 (86%) belonged to the Rhipicephalus bursa species and 91 (14%) to Hyalomma marginatum . The 91 H . marginatum ticks were organized in 27 pools, of which one (3.7%) was found positive for the presence of E . minasensis ; this pool consisted of six ticks collected from a cow bred and raised northwestern Corsica. Ehrlichial DNA was not detected in R . bursa ticks. The 16S rRNA and groEL gene sequences of Ehrlichia detected in the H . marginatum pool showed 100% (303/303 bp) and 99.8% (555/556) of nucleotide identity with E . minasensis, respectively. Phylogenetic analyses demonstrated the highest closeness with E . minasensis UFMG‐EV genotype than to any other E . canis strains. To our knowledge, this is the first report of E . minasensis outside of Brazil, Ethiopia and Canada. This identification of E . minasensis in H . marginatum merits to be further investigated and pleads for translational studies addressing the potential impact of vector‐borne diseases of human and veterinary impact through large‐scale research and surveillance programmes in Corsica.

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Ehrlichiosis

Aguiar¹

2017

Emerging and Re-Emerging Infectious Diseases of Livestock

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A novel Ehrlichia genotype detected in naturally infected cattle in North America

Cited by 38 publications

References 18 publications

Serological cross-reactivity between Anaplasma marginale and an Ehrlichia species in naturally and experimentally infected cattle

Serological cross-reactivity between Anaplasma marginale and an Ehrlichia species in naturally and experimentally infected cattle

First detection of Ehrlichia minasensis in Hyalomma marginatum ticks collected from cattle in Corsica, France

Ehrlichiosis

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