A novel dual-excitation and dual-emission fluorescent probe (CQDs-O-NBD) based on carbon quantum dots for detection and discrimination of Cys/Hcy and GSH/H2S in living cells

Zhang, Jie; Jia, Haishuang; Liu, Wenfang; Fang, Dawei

doi:10.1016/j.dyepig.2021.109554

Cited by 21 publications

(14 citation statements)

References 70 publications

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“…The CDs-based fluorescent biothiols assay was illustrated by (1) the CD–metal ion pair system [ 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 ] and (2) CD-nanocomposites [ 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 ,…”

Section: Representative Mechanism Of Cds-based Fluorescent Biothiols ...mentioning

confidence: 99%

“…In general, CD–nanocomposites produced by compositing inorganic nanomaterials (such as Au NPs, Ag NPs, and MnO 2 , etc.) and few organic molecule functionalized composite models [ 125 , 126 , 127 , 128 , 129 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 ] follows the reaction-based mechanism-2 ( Figure 3 B). On the other hand, few organic moiety functionalized CD–nanocomposite models [ 142 , 143 , 144 ] follows the reaction-based mechanism-1 ( Figure 3 A).…”

Section: Representative Mechanism Of Cds-based Fluorescent Biothiols ...mentioning

confidence: 99%

“…Thereafter, blue emissive carbon quantum dots (CQDs-OH synthesized via the microwave method; average size = 5.20 ± 0.93 nm; λ em = 467 nm at excitation of 377 nm; PLQY = 19.3%) were conjugated with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) to afford weakly fluorescent CQD–O–NBD (a composite model) and were engaged in dual channel discrimination of Cys/Hcy and GSH/H 2 S [ 128 ]. The weak fluorescent nature of CQD–O–NBD was attributed to the static quenching (SQ) and the photo-induced electron transfer (PET) from CQDs to NBD.…”

Section: CD Incorporated Nanocomposites For Biothiols Detectionmentioning

confidence: 99%

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Review on Carbon Dot-Based Fluorescent Detection of Biothiols

Shellaiah

Sun

2023

Biosensors

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Biothiols, such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), play a vital role in gene expression, maintaining redox homeostasis, reducing damages caused by free radicals/toxins, etc. Likewise, abnormal levels of biothiols can lead to severe diseases, such as Alzheimer’s disease (AD), neurotoxicity, hair depigmentation, liver/skin damage, etc. To quantify the biothiols in a biological system, numerous low-toxic probes, such as fluorescent quantum dots, emissive organic probes, composited nanomaterials, etc., have been reported with real-time applications. Among these fluorescent probes, carbon-dots (CDs) have become attractive for biothiols quantification because of advantages of easy synthesis, nano-size, crystalline properties, low-toxicity, and real-time applicability. A CDs-based biothiols assay can be achieved by fluorescent “Turn-On” and “Turn-Off” responses via direct binding, metal complex-mediated detection, composite enhanced interaction, reaction-based reports, and so forth. To date, the availability of a review focused on fluorescent CDs-based biothiols detection with information on recent trends, mechanistic aspects, linear ranges, LODs, and real applications is lacking, which allows us to deliver this comprehensive review. This review delivers valuable information on reported carbon-dots-based biothiols assays, the underlying mechanism, their applications, probe/CDs selection, sensory requirement, merits, limitations, and future scopes.

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Section: Representative Mechanism Of Cds-based Fluorescent Biothiols ...mentioning

confidence: 99%

Section: Representative Mechanism Of Cds-based Fluorescent Biothiols ...mentioning

confidence: 99%

Section: CD Incorporated Nanocomposites For Biothiols Detectionmentioning

confidence: 99%

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Review on Carbon Dot-Based Fluorescent Detection of Biothiols

Shellaiah

Sun

2023

Biosensors

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“…Wang et al obtained a “turn-on-off-on” fluorescence switch based on quantum dots and gold nanoparticles, which was utilized in ovotransferrin detection of egg powder [ 25 ]. Zhang et al reported a new dual-excitation and dual-emission fluorescent probe based on carbon quantum dots for the detection of cysteine, homocysteine, glutathione and hydrogen sulfide in living cells [ 26 ]. However, fluorescent detection on LF with carbon dots has been rarely reported so far.…”

Section: Introductionmentioning

confidence: 99%

N, S-Doped Carbon Dots Prepared by Peanut Protein Isolates and Cysteamine as Highly Sensitive Fluorescent Sensors for Fe2+, Fe3+ and Lactoferrin

Wang

et al. 2022

Polymers

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Lactoferrin (LF) is an iron-binding glycoprotein with various biological activities that has been extensively used in food and medical applications. Several methods for detecting LF have been reported, but they still face challenges in terms of sensitivity and simplicity of detection. To achieve an accurate and efficient detection of LF, we developed a method for the determination of LF in lactoferrin supplements using carbon dots (CDs) fluorescent probes. The N, S-doped PPI carbon dots (N, S-PPI-CDs) were prepared using a protein (peanut protein isolate) and cysteamine as precursors. The prepared N, S-PPI-CDs exhibited intense blue fluorescence and good biocompatibility, while the fluorescence intensity of the N, S-PPI-CDs showed a good linear relationship with Fe2+/Fe3+ concentration (0–2 μM). The N, S-PPI-CDs exhibited a high potential ability to rapidly detect Fe2+/Fe3+ within 30 s, with a limit of detection (LoD) of 0.21 μM/0.17 μM. Due to the reversible binding of LF to Fe, the N, S-PPI-CDs showed a high sensitivity and selectivity for LF, with a limit of detection (LoD) of 1.92 μg/mL. In addition, LF was quantified in real sample LF supplements and showed a fluctuation in recovery of less than 2.48%, further demonstrating the effectiveness of the fluorescent N, S-PPI-CDs sensor.

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“…Owing to their superior properties of high photostability, easy surface functionalization, good biocompatibility, low toxicity, and facile synthesis, CDs have good application prospects in various elds, including chemical sensing, medical diagnosis, drug delivery, bio-imaging, catalysis, and optoelectronic devices. [1][2][3][4][5] There are a variety of approaches to prepare CDs, such as hydrothermal, solvothermal, ultrasound treatment, and microwave methods. With the advantages of simple equipment, short reaction time, and low cost, the microwave route has attracted increasing interest for synthesizing CDs.…”

Section: Introductionmentioning

confidence: 99%