A novel dodecapeptide from a combinatorial synthetic library exhibits potent antifungal activity and synergy with standard antimycotic agents

Duggineni, Srinivas; Srivastava, Gaurav; Kundu, Bijoy; Kumar, Manish; Chaturvedi, A. K.; Shukla, Praveen Kumar

doi:10.1016/j.ijantimicag.2006.08.038

Cited by 8 publications

(9 citation statements)

References 17 publications

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“…Resistance against fungicidal antifungal agents can be assayed by calculating the CFU reduction at different time intervals. The time dependent antifungal activity can be assessed by time kill curve (Duggineni et al 2007). Amphotericin B concentration used in the study was MIC (0.06 mg/ml) of the parent strain whereas for the AMB-R strain it was 128 mg/ml.…”

Section: Resultsmentioning

confidence: 99%

“…One millilitre of the inoculum was added to 9 ml of RPMI 1640 medium buffered with MOPS. Amphotericin B was added at different concentration in RPMI 1640 medium as per MIC for the parent as well as the resistant strain and incubated in rotary shaker (50 rpm) at 35 C. One hundred microlitres of the broth was taken out after 0, 4, 8, 16, 24, and 48 h of incubation and the CFU was determined by serial dilution and plating method on SDA plates in triplicates (Duggineni et al 2007). …”

Section: Time Kill Assaymentioning

confidence: 99%

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Amphotericin B resistance leads to enhanced proteinase and phospholipase activity and reduced germ tube formation in Candida albicans

Kumar

Shukla

2010

Fungal Biology

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Section: Resultsmentioning

confidence: 99%

Section: Time Kill Assaymentioning

confidence: 99%

Amphotericin B resistance leads to enhanced proteinase and phospholipase activity and reduced germ tube formation in Candida albicans

Kumar

Shukla

2010

Fungal Biology

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“…N. gonorrhea and S. aureus 31. There were earlier studies on the combined use of antimicrobial, antifungal and antiviral peptides to inhibit microbial growth in combination with conventionally used antibiotics or drugs37–39. However, to our knowledge this is the first report on the nature of interaction and ability of reproductive tract antimicrobial proteins and peptides to kill bacteria in combination with conventionally used antibiotics.…”

Section: Discussionmentioning

confidence: 99%

The human male reproductive tract antimicrobial peptides of the HE2 family exhibit potent synergy with standard antibiotics

Yenugu

Narmadha

2010

Journal of Peptide Science

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Reproductive tract infections pose a serious threat to health and fertility. Due to the emergence of antibiotic resistant pathogens, antimicrobial proteins and peptides of the reproductive tract are extensively characterized in recent years toward developing newer strategies to treat genital tract infections. Pathogen growth inhibition using a combination of naturally occurring male reproductive tract antimicrobial peptides and commonly used antibiotics has not been reported. Checker board analyses were carried out to determine the nature of interaction (synergistic, additive and antagonistic) between HE2alpha and HE2beta2 peptides and the commonly used antibiotics. Using Escherichia coli as the target organism, the minimal inhibitory concentration and fractional inhibitory concentration indices were determined. We demonstrate for the first time that the human male reproductive tract antimicrobial peptides HE2alpha and HE2beta2 act synergistically with the commonly used antibiotics to inhibit E. coli growth. A combination of HE2alpha and HE2beta2 peptides resulted in an additive effect. Interestingly, the synergistic effects of HE2 peptides were highest with doxycycline and ciprofloxacin, antibiotics generally used to treat epididymitis. Results of this study demonstrate the potential of endogenous HE2 peptides to be pharmacologically important in designing novel strategies to treat reproductive tract infections.

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“…A large number of publications have described in vitro synergy of selected AMP with other AMP (Zerweck et al, 2017 ; Hanson et al, 2019 ), antibiotics (for example Cassone and Otvos, 2010 ; Sakoulas et al, 2014 ; Soren et al, 2015 ; Pollini et al, 2017 ; Pizzolato-Cezar et al, 2019 ) or antifungals (Duggineni et al, 2007 ; Singh et al, 2017 ), although this is not always the case (He et al, 2015 ) and is something that is no doubt under-reported. Conjugates of AMP and antibiotics, organometallic compounds, gold nanoparticles or to create AMP polymers to increase efficacy, to reduce toxicity and/or to improve formulation have become of increasing interest over recent years (for examples, see Reinhardt and Neundorf, 2016 ; Rajchakit and Sarojini, 2017 ; David et al, 2018 ; Sun et al, 2018 ) and will undoubtedly require additional consideration when it comes to AST.…”

Section: Antimicrobial Susceptibility Testing Of Ampmentioning

confidence: 99%

Antimicrobial Susceptibility Testing of Antimicrobial Peptides to Better Predict Efficacy

Mercer

Torres

Duay

et al. 2020

Front. Cell. Infect. Microbiol.

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During the development of antimicrobial peptides (AMP) as potential therapeutics, antimicrobial susceptibility testing (AST) stands as an essential part of the process in identification and optimisation of candidate AMP. Standard methods for AST, developed almost 60 years ago for testing conventional antibiotics, are not necessarily fit for purpose when it comes to determining the susceptibility of microorganisms to AMP. Without careful consideration of the parameters comprising AST there is a risk of failing to identify novel antimicrobials at a time when antimicrobial resistance (AMR) is leading the planet toward a post-antibiotic era. More physiologically/clinically relevant AST will allow better determination of the preclinical activity of drug candidates and allow the identification of lead compounds. An important consideration is the efficacy of AMP in biological matrices replicating sites of infection, e.g., blood/plasma/serum, lung bronchiolar lavage fluid/sputum, urine, biofilms, etc., as this will likely be more predictive of clinical efficacy. Additionally, specific AST for different target microorganisms may help to better predict efficacy of AMP in specific infections. In this manuscript, we describe what we believe are the key considerations for AST of AMP and hope that this information can better guide the preclinical development of AMP toward becoming a new generation of urgently needed antimicrobials.

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A novel dodecapeptide from a combinatorial synthetic library exhibits potent antifungal activity and synergy with standard antimycotic agents

Cited by 8 publications

References 17 publications

Amphotericin B resistance leads to enhanced proteinase and phospholipase activity and reduced germ tube formation in Candida albicans

Amphotericin B resistance leads to enhanced proteinase and phospholipase activity and reduced germ tube formation in Candida albicans

The human male reproductive tract antimicrobial peptides of the HE2 family exhibit potent synergy with standard antibiotics

Antimicrobial Susceptibility Testing of Antimicrobial Peptides to Better Predict Efficacy

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