A novel cytosensor based on Pt@Ag nanoflowers and AuNPs/Acetylene black for ultrasensitive and highly specific detection of Circulating Tumor Cells

Tang, Sitian; Shen, Huawei; Hao, Yixiong; Huang, Zhenglan; Tao, Yiyi; Yang, Peng; Guo, Yongcan; Xie, Guoming; Feng, Wenli

doi:10.1016/j.bios.2018.01.001

Cited by 74 publications

(27 citation statements)

References 32 publications

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“…AuNPs/AB nanomaterial was used as a substrate material to increase the specific surface area and conductivity of the gold electrode. The detection range of the sensor was 20 to 1.0 x 10 6 cells/mL, and the detection limit was 3 cells/mL . In addition, researchers have cleverly combined photoexcitation and electrochemical detection processes to construct photoelectrochemical (PEC) biosensors with higher detection sensitivity for the detection of circulating tumor cells.…”

Section: Detection Of Circulating Tumor Cells By Electrochemical Biosmentioning

confidence: 99%

“…23 Tang et al designed a novel ultrasensitive immunoassay protocol by using Pt@Ag nanoflowers (pt@AgNFs) and AuNPs/Acetylene black (AuNPs/AB) nanomaterial to detect CTCs. 17 Pt@AgNFs had high specific surface area and good biocompatibility, and were not only used as the carriers of signal antibodies (Ab 2 ) but also catalyzed the reduction of H 2 O 2 to amplify the current signal. AuNPs/AB nanomaterial was used as a substrate material to increase the specific surface area and conductivity of the gold electrode.…”

Section: Detection Of Circulating Tumor Cells By Electrochemical Biosmentioning

confidence: 99%

“…The detection range of the sensor was 20 to 1.0 x 10 6 cells/mL, and the detection limit was 3 cells/mL. 17 In addition, researchers have cleverly combined photoexcitation and electrochemical detection processes to construct photoelectrochemical (PEC) biosensors with higher detection sensitivity for the detection of circulating tumor cells. In this study, a PEC biosensor was proposed based on hexagonal carbon nitride tubes (HCNT) as photosensitive material, and magnetic Fe3O4 nanospheres were used for efficient magnetic capture of CTCs, and Cu2O nanoparticles were used for signal amplification, making the detection range of this sensor range from 3 to 3000 cells/mL, with a detection limit down to 1 cell/mL.…”

Section: Detection Of Circulating Tumor Cells By Electrochemical Biosmentioning

confidence: 99%

See 2 more Smart Citations

Application of electrochemical biosensors in tumor cell detection

Zhang

et al. 2020

Thoracic Cancer

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Conventional methods for detecting tumors, such as immunological methods and histopathological diagnostic techniques, often request high analytical costs, complex operation, long turnaround time, experienced personnel and high false‐positive rates. In addition, these assays are difficult to obtain an early diagnosis and prognosis quickly for malignant tumors. Compared with traditional technology, electrochemical technology has realized the study of interface charge transfer behavior at the atomic and molecular levels, which has become an important analytical and detection tool in contemporary analytical science. Electrochemical technique has the advantages of rapid detection, high sensitivity (single cell) and specificity in the detection of tumor cells, which has not only been successful in differentiating tumor cells from normal cells, but has also achieved targeted detection of localized tumor cells and circulating tumor cells. Electrochemical biosensors provide powerful tools for early diagnosis, staging and prognosis of tumors in clinical medicine. Therefore, this review mainly discusses the development and application of electrochemical biosensors in tumor cell detection in recent years.

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Section: Detection Of Circulating Tumor Cells By Electrochemical Biosmentioning

confidence: 99%

Section: Detection Of Circulating Tumor Cells By Electrochemical Biosmentioning

confidence: 99%

Section: Detection Of Circulating Tumor Cells By Electrochemical Biosmentioning

confidence: 99%

See 1 more Smart Citation

Application of electrochemical biosensors in tumor cell detection

Zhang

et al. 2020

Thoracic Cancer

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“…The principle of electrochemical detection is to modify the electrode using nanomaterials and cancer cell specific aptamer, and CTC is bonded to aptamer (on the electrode surface), which will cause the current/electrochemiluminescence (ECL)/photoelectrochemical (PEC) signal to decrease or EC impedance spectroscopy (EIS) signal to increase [27,[92][93][94][95][96]. By establishing a linear relationship between the change and the number of CTC, the quantification can be realized.…”

Section: Electrochemical Detection Of Ctcmentioning

confidence: 99%

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Han

Sun

et al. 2020

Sensors

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Circulating tumor cells (CTCs) are tumor cells that escaped from the primary tumor or the metastasis into the blood and they play a major role in the initiation of metastasis and tumor recurrence. Thus, it is widely accepted that CTC is the main target of liquid biopsy. In the past few decades, the separation of CTC based on the electrochemical method has attracted widespread attention due to its convenience, rapidness, low cost, high sensitivity, and no need for complex instruments and equipment. At present, CTC detection is not widely used in the clinic due to various reasons. Point-of-care CTC detection provides us with a possibility, which is sensitive, fast, cheap, and easy to operate. More importantly, the testing instrument is small and portable, and the testing does not require specialized laboratories and specialized clinical examiners. In this review, we summarized the latest developments in the electrochemical-based CTC detection and point-of-care CTC detection, and discussed the challenges and possible trends.

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“…[31][32][33][34] Silver nanoowers have large specic surface area and good electrical conductivity, especially peroxidase-like properties, which provides an excellent substrate for the construction of the assay. [35][36][37] A new type of Pt@Ag nanoower (Pt@Ag NFs) was prepared by adhesion of platinum nanoparticles with high catalytic activity on the surface of silver nanoowers to further improve the ability of reducing H 2 O 2 .…”

Section: Introductionmentioning

confidence: 99%