A novel cysteine-rich antimicrobial peptide from the mucus of the snail of Achatina fulica

Zhong, Jian; Wang, Wenhong; Yang, Xiaomei; Yan, Xiuwen; Li, Rui

doi:10.1016/j.peptides.2012.09.001

Cited by 66 publications

(75 citation statements)

References 24 publications

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“…aureus does concur with previous work 13,14,15,16,17 , although none of the other authors report the relatively weak effect found in the present study. However, apart from Santana et al 17 , all describe isolating the active ingredient -i.e.…”

Section: Discussionsupporting

confidence: 91%

“…However, apart from Santana et al 17 , all describe isolating the active ingredient -i.e. Achacin 13,14,15 or Mytimycin-AF 16 -before testing it against the bacteria. Santana et al 17 , seem to have followed a very similar antimicrobial assay method to that employed in the present study, with the exception of their use of Mueller -Hinton agar instead of ISA and wells in the agar instead of discs.…”

Section: Discussionmentioning

confidence: 99%

“…Initially this finding was rather unexpected, since components of A.fulica mucous have previously been reported to inhibit the growth of E.coli 15,16 , a number of Bacillus species 13,16 and K.pneumoniae 16 as well as C.albicans 16 . Extrapolating from the literature it seemed possible that H.aspersa mucous might have a similar broad spectrum of activity, which is why a variety of organisms were tested in the initial antimicrobial assay.…”

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confidence: 99%

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Antimicrobial properties of mucus from the brown garden snail Helix aspersa

Pitt

Graham²,

Dedi

et al. 2015

British Journal of Biomedical Science

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Research into naturally occurring antimicrobial substances has yielded effective treatments. One area of interest is peptides and proteins produced by invertebrates as part of their defence system, including the contents of mollusc mucous. Mucous produced by the African giant land snail, Achatina fulica has been reported to contain two proteins with broad spectrum anti-bacterial activity. Mucous from the brown garden snail, Helix aspersa appears to have skin regenerationproperties. This study sought to investigate the antimicrobial properties of H.aspersa mucous.Mucous was collected from H.aspersa snails, diluted in PBS and centrifuged, with the supernatant tested against a wide range of organisms in a disc diffusion antimicrobial assay. This was followed up with comparative experiments involving A.fulica , including bacteriophage assays. Mucous from both species of snail was passed through a series of protein size separation columns in order to determine the approximate size of the antimicrobial substance. Electrophoresis was also carried out on the H.aspersa mucous.Results indicated that H.aspersa mucous had a strong antibacterial effect against several strains of Pseudomonas aeruginosa and a weak effect against Staphylococcus aureus. Mucous from A.fulica also inhibited the growth of S.aureus, but the broad spectrum of activity reported by other workers was not observed. Antimicrobial activity was not caused by bacteriophage. Size separation experiments indicated that the antimicrobial substance(s) in H.aspersa were between 30 and 100 kDa. Electrophoresis revealed two proteins in this region -30-40 kDa and 50-60kDa. These do not correspond with antimicrobial proteins previously reported in A.fulica. This study found one or more novel antimicrobial agents in H.aspersa mucous, with a strong effect against P.aeruginosa . 2

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Antimicrobial properties of mucus from the brown garden snail Helix aspersa

Pitt

Graham²,

Dedi

et al. 2015

British Journal of Biomedical Science

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“…Tales polisacáridos son referidos como mucopolisacaridos que son el mayor constituyente de estas secreciones y del cual se han propuesto un número importante de funciones biológicas como: la inhibición de la angiogénesis en los procesos inflamatorios por medio de acharan sulfato 10 ; la inhibición del crecimiento microbiano debido a la presencia de péptidos y lectinas involucradas en la defensa inmunológica de estos moluscos 11,12,13 . Existen otros componentes como la alantoína que tiene propiedades estimulantes de la epitelización de la piel por estímulo de la proliferación de fibroblastos 14 , el ácido glucónico que actúa como exfoliante natural de la piel, el colágeno y la elastina que confieren tersura a la piel; y enzimas fibrinolíticas que limpian los microcapilares favoreciendo la oxigenación y nutrición de los tejidos, y con ello un remodelamiento vascular adecuado 15,16,17 .…”

Section: Introductionunclassified

“…Aunque no se han hecho estudios detallados sobre las características físicas y bioquímicas de la secreción mucosa sí se han identificado Péptidos Antimicrobianos (PAMs) que pueden constituir una alternativa al problema de la resistencia que presentan muchos microorganismos 13,18 . El estudio de la secreción mucosa es la mejor manera de convertir un problema en oportunidades para el avance científico con miras a obtener productos que solucionen necesidades de importancia biomédica, teniendo en cuenta sus propiedades cosméticas, cicatrizantes y antimicrobianas demostradas.…”

Section: Introductionunclassified

Caracterización físico-química y actividad antimicrobiana de la secreción mucosa de Achatina fulica

Pereira¹,

P²,

R³

et al. 2016

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Microbial diversity of garden snail mucus

et al. 2022

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The search for new natural compounds for application in medicine and cosmetics is a trend in biotechnology. One of the sources of such active compounds is the snail mucus. Snail physiology and the biological activity of their fluids (especially the mucus) are still poorly studied. Only a few previous studies explored the relationship between snails and their microbiome. The present study was focused on the biodiversity of the snail mucus used in the creation of cosmetic products, therapeutics, and nutraceuticals. The commonly used cultivation techniques were applied for the determination of the number of major bacterial groups. Fluorescence in situ hybridization for key taxa was performed. The obtained images were subjected to digital image analysis. Sequencing of the 16S rRNA gene was also done. The results showed that the mucus harbors a rich bacterial community (10.78 × 1010 CFU/ml). Among the dominant bacteria, some are known for their ability to metabolize complex polysaccharides or are usually found in soil and plants (Rhizobiaceae, Shewanella, Pedobacter, Acinetobacter, Alcaligenes). The obtained data demonstrated that the snail mucus creates a unique environment for the development of the microbial community that differs from other parts of the animal and which resulted from the combined contribution of the microbiomes derived from the soil, plants, and the snails.

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A novel cysteine-rich antimicrobial peptide from the mucus of the snail of Achatina fulica

Cited by 66 publications

References 24 publications

Antimicrobial properties of mucus from the brown garden snail Helix aspersa

Antimicrobial properties of mucus from the brown garden snail Helix aspersa

Caracterización físico-química y actividad antimicrobiana de la secreción mucosa de Achatina fulica

Microbial diversity of garden snail mucus

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