A novel combination of immobilized Enterococcus casseliflavus sp. nov. with silver nanoparticles into a reusable matrix of Ca-Alg beads as a new strategy for biotreatment of Disperse Blue 183: Insights into metabolic characterization, biotoxicity, and mutagenic properties

Nazari, Negin; Kashi, Fereshteh Jookar

doi:10.1016/j.jenvman.2022.116578

Cited by 5 publications

(5 citation statements)

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“…Overall, the production of beads for cell immobilization by AM, as conjectured in the previous literature [13], proved to be viable using the two different material/process combinations. The results represent a remarkable advancement, as they open up the possibility of designing and producing complex geometries with diverse exchange surfaces, which could not be accomplished using the traditional methods [1,14,28,29]. As compared To summarize the feasibility of the beads made using PBF, the manufacturing limits are primarily attributable to the unconsolidated powder-emptying.…”

Section: Discussionmentioning

confidence: 99%

“…Both bead typologies produced by PBF and vat photopolymerization were investigated using scanning electron microscopy (SEM) [28,29] and X-ray micro-computed tomography (XmCT). The surface morphology of the vat photopolymerized beads in charged and uncharged conditions was observed by SEM with a Tescan Vega 3 (Tescan Company, Brno, Czech Republic).…”

Section: Characterizationmentioning

confidence: 99%

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Beads for Cell Immobilization: Comparison of Alternative Additive Manufacturing Techniques

et al. 2023

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The attachment or entrapment of microbial cells and enzymes are promising solutions for various industrial applications. When the traps are beads, they are dispersed in a fluidized bed in a vessel where a pump guarantees fresh liquid inflow and waste outflow without washing out the cells. Scientific papers report numerous types of cell entrapment, but most of their applications remain at the laboratory level. In the present research, rigid polymer beads were manufactured by two different additive manufacturing (AM) techniques in order to verify the economy, reusability, and stability of the traps, with a view toward a straightforward industrial application. The proposed solutions allowed for overcoming some of the drawbacks of traditional manufacturing solutions, such as the limited mechanical stability of gel traps, and they guaranteed the possibility of producing parts of constant quality with purposely designed exchange surfaces, which are unfeasible when using conventional processes. AM proved to be a viable manufacturing solution for beads with complex shapes of two different size ranges. A deep insight into the production and characteristics of beads manufactured by AM is provided. The paper provides biotechnologists with a manufacturing perspective, and the results can be directly applied to transit from the laboratory to the industrial scale.

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Section: Discussionmentioning

confidence: 99%

Section: Characterizationmentioning

confidence: 99%

Beads for Cell Immobilization: Comparison of Alternative Additive Manufacturing Techniques

et al. 2023

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“…Artemia eggs were suspended in artificial seawater solution and incubated for 48 h in an aquarium with pH 9, ambient temperature, and constant agitation using an air pump. Then, 10 larvae were shifted to glass tubes containing saline water with increasing concentrations (0, 0.01, 0.1, 0.3, 0.5, 0.7, 1 mg/mL) of RB19 and post‐treatment products and incubated for another 24 h. Artificial seawater and vincristine sulfate were used parallelly acting as positive and negative controls (Nazari & Kashi, 2023). All the experiments were conducted three times in succession.…”

Section: Methodsmentioning

confidence: 99%

“…Then, a mixture of bacterial suspension in the log growth phase and Na-alginate solution was designed and introduced dropwise into the CaCl 2 3% solution. As soon as the drops came into contact with the CaCl 2 solution, The gel beads formed and were stored overnight for more hardening (Nazari & Kashi, 2023). The inside and outside morphology of immobilized gel beads at a microscopic scale was investigated by scanning electron microscopy (TESCAN Mira 3).…”

Section: Immobilization and Decolorization Experimentsmentioning

confidence: 99%

Improved application of immobilized Enterobacter cloacae into a bio‐based polymer for Reactive Blue 19 removal, an eco‐friendly advancement in potential decolorizing systems

Nazari,

Jookar Kashi,

Nazari

2024

Water Environment Research

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The widespread use of highly complex synthetic dyes like reactive dyes in the textile industry has some adverse environmental impacts and deserves close attention. Biological treatment of these effluents utilizing various species of bacteria with remarkable efficiency in dye removal is still considered promising. Our current study deals with immobilizing an isolated bacterial strain into calcium alginate (Ca/Alg) gel beads and using it to treat pernicious pollutants like synthetic dyes. A potential Reactive Blue 19 (RB19)‐degrading Enterobacter cloacae strain A1 was isolated from the Kashan textile industry and was characterized by 16S rDNA gene sequencing. The decolorization ability of strain A1 was assessed by time‐based studies using free bacterial cells/immobilized in Ca/Alg. Based on the results of the 16S rDNA gene sequencing, it appears that strain A1 belonged to E. cloacae, with a 99.74% similarity. The findings suggest that immobilized strain A1 accomplished maximum decolorization activity compared with the free cells. The immobilized strain could utterly decompose and decolorize 0.05 mg/mL of RB19 within 48 h, while the free bacterial strain decolorized RB19 within 5 days. Moreover, Ca/Alg gel beads can maintain their efficiency for over three decolorization cycles. Further infrared spectroscopy (FTIR) and gas chromatograph mass spectrometer (GC/MS) investigation declared complete RB19 decomposition on reaction products. Artemia salina was used to investigate the toxicity of dye and its degraded metabolites. The LC50 values signified the pure dye as very toxic with 0.01 mg/mL concentration, while after‐treatment products showed no toxic effect on larvae. This immobilization technique increased the applicability of bacterial strain for dye removal. It was beneficial for the decolorization of RB19 from textile wastewater due to a remarkable reduction in time. Notably, strain A1‐immobilized beads can maintain their activity for three consecutive decolorization cycles without a considerable decrease in efficiency.Practitioner Points The remarkable capacity of immobilized Enterobacter cloacae strain A1 for Reactive Blue 19 (RB19) removal Immobilized A1 strain showed two‐fold higher removal than free one over 48 h A promising method for enhancing RB19 decolorization Decolorization was due to degradation based on UV–Vis, FTIR, and GC/MS analysis Non‐toxic posttreatment products for Artemia

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“…This natural biofilm can degrade higher concentrations of dyes and withstand adverse conditions otherwise not possible by discrete microbes [26]. Hence, Enterobacter sps may be considered as the most feasible and eco-friendly bio-remediator for reactive dye biotransformation to nontoxic end products [27,28].…”

Section: Introductionmentioning

confidence: 99%

Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites

Lakshmaiah,

Nag,

Gotekar

et al. 2023

J App Biol Biotech

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Reactive Blue 222 (RB222) is widely used in textile industries and hence a common recalcitrant pollutant in the industrial effluent. Bioremediation of this dye is of significance as its one of the complex dyes with high molecular weight. In the present study, we isolated a novel bacterial strain Enterobacter CU2004 from the industrial waste and characterize using16S rRNA gene sequencing. Its potential to dye degradation was evaluated in a simple minimal salt media with the parameters namely dye concentration (100-1000 ppm), pH (4-9), temperature (15-55°C), Carbon source (Lactose, Sucrose, Glucose, Starch, and Fructose), and Nitrogen source (Casein, Yeast extract, Peptone, Tryptone, Ammonium sulphate, and Urea) in a 24 h culture. Finally, data obtained were extended to design of experiment based optimization for the degradation efficacy of Enterobacter CU2004 and to validated design space was established. The novelty is in optimizing the design space parameters for highest percentage of degradation ≥90% by the bacterial isolate Enterobacter CU2004 were finalized as 30-37°C temperature, 133-249 ppm dye concentration, Lactose as Carbon source, Yeast extract as Nitrogen source, and the pH as 8. Microbial dye degradation was confirmed by FTIR, HPLC and GCMS studies. Further studies revealed the dye intermediates and the potential of Enterobacter CU2004 toward the degradation of complex, high molecular weight industrial dye RB222.

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A novel combination of immobilized Enterococcus casseliflavus sp. nov. with silver nanoparticles into a reusable matrix of Ca-Alg beads as a new strategy for biotreatment of Disperse Blue 183: Insights into metabolic characterization, biotoxicity, and mutagenic properties

Cited by 5 publications

References 58 publications

Beads for Cell Immobilization: Comparison of Alternative Additive Manufacturing Techniques

Beads for Cell Immobilization: Comparison of Alternative Additive Manufacturing Techniques

Improved application of immobilized Enterobacter cloacae into a bio‐based polymer for Reactive Blue 19 removal, an eco‐friendly advancement in potential decolorizing systems

Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites

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