A novel co-cultivation strategy to generate low-crystallinity bacterial cellulose and increase nisin yields

Qiao, Wanjin; Qiao, Yu; Liao, Zitong; Gao, Ge; Wu, Zhenzhou; Saris, Per E. J.; Xu, Haijin; Qiao, Mingqiang

doi:10.1016/j.ijbiomac.2022.01.038

Cited by 8 publications

(4 citation statements)

References 48 publications

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“…For this reason, instead of using purified materials, in recent years, multi-microbial systems, namely co-culture, have emerged, as cost-effective strategies. In a microbial co-culture system, microorganisms are cultivated together, obtaining combined composites, directly during microbial growth [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

A Microbial Co-Culturing System for Producing Cellulose-Hyaluronic Acid Composites

et al. 2023

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In this study, a co-culture system combining bacterial cellulose (BC) producers and hyaluronic acid (HA) producers was developed for four different combinations. AAB of the genus Komagataeibacter sp. and LAB of the Lactocaseibacillus genus were used to produce BC and HA, respectively. Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction were used to investigate changes in BC-HA composites chemical and morphological structure. Water absorption, uptake, and antibacterial properties were also tested. Outcomes highlighted a higher bacterial cellulose yield and the incorporation of hyaluronic acid into the composite. The presence of hyaluronic acid increased fiber dimension—nearly doubled for some combinations—which led to a decreased crystallinity of the composites. Different results were observed based on the BC producer and HA producer combination. However, water holding capacity (WHC) in all the samples improved with the presence of HA, while water uptake worsened. A thymol-enriched BC-HA composite showed high antibacterial activity against Escherichia coli DSM 30083T and Staphylococcus aureus DSM 20231T. Results could contribute to opening new applications in the cosmetics or pharmaceutical fields.

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Section: Introductionmentioning

confidence: 99%

A Microbial Co-Culturing System for Producing Cellulose-Hyaluronic Acid Composites

et al. 2023

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“…5C). This low-crystallinity BC shares diverse advantages especially in biomedical or cosmetics areas [40]. For example, BC with low crystallinity results in high rehydration and water holding capacity, which helps to absorb tissue fluid and blood exuded from the wound to achieve hemostasis [41].…”

Section: Discussionmentioning

confidence: 99%

Inducible biosynthesis of bacterial cellulose in recombinantEnterobactersp. FY-07

Ren,

Miao,

Feng

et al. 2024

Preprint

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Bacterial cellulose (BC) is an extracellular polysaccharide with myriad unique properties, such as high purity, water-holding capacity and biocompatibility, making it attractive in materials science. However, genetic engineering techniques for BC-producing microorganisms are rare. Herein, the electroporation-based gene transformation and the λ Red-mediated gene knockout method with a nearly 100% recombination efficiency were established in the fast-growing and BC hyperproducerEnterobactersp. FY-07. This genetic manipulation toolkit was validated by inactivating the protein subunit BcsA in the cellulose synthase complex. Subsequently, the inducible BC-producing strains from glycerol were constructed through inducible expression of the key genefbpin the gluconeogenesis pathway, which recovered more than 80% of the BC production. Finally, the BC properties analysis results indicated that the induced-synthesized BC pellicles were looser, more porous and reduced crystallinity, which could further broaden the application prospects of BC. To our best knowledge, this is the first attempt to construct the completely inducible BC-producing strains. Our work paves the way for increasing BC productivity by metabolic engineering and broadens the available fabrication methods for BC-based advanced functional materials.

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“…The 2θ scans were made in the range between 5° and 40° at a speed of 5°/min. The degree of crystallinity (crystallinity index, CrI) was determined by the following equation: CrI(%) = (I 002 − I AM )/I 002 × 100%, where I 002 represents the maximum intensity of the lattice diffraction at approximately 2θ = 22.6°, and I AM represents the diffraction intensity in the same units at approximately 2θ = 18° [ 22 ]. Thermogram analysis, which was used to investigate the thermal stability of the BC membranes [ 23 ], was conducted using a thermal analyzer (Q600 SDT TGA, TA, NewCastle, DE, USA) at a heating rate of 10 °C/min.…”

Section: Methodsmentioning

confidence: 99%

Production of Bacterial Cellulose in the Medium with Yeasts Pre-Fermented Coconut Water or with Addition of Selected Amino Acids

Lin

Song

Jiang

et al. 2022

Foods

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The uncontrolled natural pre-fermentation process of coconut water represents great hidden safety hazards, unstable production, and impact on the quality of nata de coco–the trade name of bacterial cellulose (BC) in food industry. In this study, BC production from Komagataeibacter nataicola Q2 was conducted in the media of coconut water (50%, v/v) pre-fermented by 11 coconut-sourced yeast strains in static. Results suggested that coconut water pre-fermented by different yeast strains had varied effects on the production of BC. Compared with the use of fresh coconut water, the use of coconut water pre-fermented by Saccharomyces cerevisiae SC7 increased the BC yield by 165%. Both natural pre-fermentation and SC7 pre-fermentation altered the concentrations of amino acids in fresh coconut water. The addition of selected amino acids aspartic acid, glutamic acid, serine, methionine, threonine, isoleucine, phenylalanine, and proline at different concentrations had varied effects on the production of BC. The yield of BC was the highest when adding 3.0% (w/v) methionine. Moreover, adding 3.0% methionine allowed the production of BC with larger loops of looser aggregated microfibers, increased the crystallinity of BC from 64.8% to 69.4%, but decreased the temperature of maximum weight loss rate, hardness, and adhesiveness from 223 °C, 8.68 kg, and 92.8 g.sec to 212 °C, 7.01 kg, and 58.5 g.sec, respectively, in the test condition.

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A novel co-cultivation strategy to generate low-crystallinity bacterial cellulose and increase nisin yields

Cited by 8 publications

References 48 publications

A Microbial Co-Culturing System for Producing Cellulose-Hyaluronic Acid Composites

A Microbial Co-Culturing System for Producing Cellulose-Hyaluronic Acid Composites

Inducible biosynthesis of bacterial cellulose in recombinantEnterobactersp. FY-07

Production of Bacterial Cellulose in the Medium with Yeasts Pre-Fermented Coconut Water or with Addition of Selected Amino Acids

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