A Microbial Co-Culturing System for Producing Cellulose-Hyaluronic Acid Composites

Brugnoli, Marcello; Mazzini, Ilaria; China, Salvatore La; Vero, Luciana De; Gullo, Maria

doi:10.3390/microorganisms11061504

Cited by 3 publications

(2 citation statements)

References 62 publications

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“…This suggests that LAB also has a role in promoting SCOBY formation. This finding is consistent with that of a previous study indicating that co-cultivation of AAB and LAB results in the increased formation of bacterial cellulose compared with AAB monoculture ( Brugnoli et al, 2023 ). Quorum sensing (QS), a density-dependent signaling mechanism, facilitates the interaction between LAB and AAB, leading to bacterial cellulose formation ( Zhang et al, 2021 ).…”

Section: Discussionsupporting

confidence: 93%

Multi-Strain Probiotics Enhance the Bioactivity of Cascara Kombucha during Microbial Composition-Controlled Fermentation

Van,

Phan,

Quang

et al. 2023

Prev. Nutr. Food Sci.

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Kombucha is a widely consumed fermented tea beverage with diverse health benefits. In a previous study, we demonstrated that the use of cascara as a substrate results in a special kombucha beverage with high bioactivity. Traditional kombucha fermentation using a symbiotic culture of bacteria and yeast (SCOBY) can lead to inconsistent product quality because of the lack of control over microbial composition. We successfully isolated and identified yeast and bacteria, including Saccharomyces cerevisiae, Komagataeibacter rhaeticus, and Lactobacillus brevis that are appropriate starter cultures for cascara kombucha fermentation. We also demonstrated that a supplementation with lactic acid bacteria (LAB) and a mixture of S. cerevisiae and K. rhaeticus resulted in higher total polyphenol and flavonoid content of cascara kombucha compared with the traditionally fermented product using SCOBY as the inoculum. The free radical scavenging activity, inhibitory effects on -amylase, tyrosinase activity, and antibacterial properties of cascara kombucha were also enhanced as a result of LAB supplement. These findings provide valuable insights into the controlled microbiological composition required for the fermentation of cascara kombucha, thereby ensuring consistent quality and enhanced bioactivity of the product. Further, the use of cascara as a substrate for kombucha production not only offers various health benefits and biological effects, but also repurposes by-products from the coffee industry, which contributes to sustainable development and is eco-friendly.

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Section: Discussionsupporting

confidence: 93%

Multi-Strain Probiotics Enhance the Bioactivity of Cascara Kombucha during Microbial Composition-Controlled Fermentation

Van,

Phan,

Quang

et al. 2023

Prev. Nutr. Food Sci.

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show abstract

“… Schematic representation of co-culture approach for obtaining BC and hyaluronic acid (HA) composite (adapted from Brugnoli et al [ 128 ] (CC BY)). …”

Section: Figures Scheme and Tablementioning

confidence: 99%

From Nature to Lab: Sustainable Bacterial Cellulose Production and Modification with Synthetic Biology

Potočnik,

Gorgieva,

Trček

2023

Polymers

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Bacterial cellulose (BC) is a macromolecule with versatile applications in medicine, pharmacy, biotechnology, cosmetology, food and food packaging, ecology, and electronics. Although many bacteria synthesize BC, the most efficient BC producers are certain species of the genera Komagataeibacter and Novacetimonas. These are also food-grade bacteria, simplifying their utilization at industrial facilities. The basic principles of BC synthesis are known from studies of Komagataeibacter xylinus, which became a model species for studying BC at genetic and molecular levels. Cellulose can also be of plant origin, but BC surpasses its purity. Moreover, the laboratory production of BC enables in situ modification into functionalized material with incorporated molecules during its synthesis. The possibility of growing Komagataeibacter and Novacetimonas species on various organic substrates and agricultural and food waste compounds also follows the green and sustainable economy principles. Further intervention into BC synthesis was enabled by genetic engineering tools, subsequently directing it into the field of synthetic biology. This review paper presents the development of the fascinating field of BC synthesis at the molecular level, seeking sustainable ways for its production and its applications towards genetic modifications of bacterial strains for producing novel types of living biomaterials using the flexible metabolic machinery of bacteria.

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Production of Bacterial Exopolysaccharides: Xanthan and Bacterial Cellulose

Revin,

Liyaskina,

Parchaykina

et al. 2023

IJMS

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Recently, degradable biopolymers have become increasingly important as potential environmentally friendly biomaterials, providing a wide range of applications in various fields. Bacterial exopolysaccharides (EPSs) are biomacromolecules, which due to their unique properties have found applications in biomedicine, foodstuff, textiles, cosmetics, petroleum, pharmaceuticals, nanoelectronics, and environmental remediation. One of the important commercial polysaccharides produced on an industrial scale is xanthan. In recent years, the range of its application has expanded significantly. Bacterial cellulose (BC) is another unique EPS with a rapidly increasing range of applications. Due to the great prospects for their practical application, the development of their highly efficient production remains an important task. The present review summarizes the strategies for the cost-effective production of such important biomacromolecules as xanthan and BC and demonstrates for the first time common approaches to their efficient production and to obtaining new functional materials for a wide range of applications, including wound healing, drug delivery, tissue engineering, environmental remediation, nanoelectronics, and 3D bioprinting. In the end, we discuss present limitations of xanthan and BC production and the line of future research.

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A Microbial Co-Culturing System for Producing Cellulose-Hyaluronic Acid Composites

Cited by 3 publications

References 62 publications

Multi-Strain Probiotics Enhance the Bioactivity of Cascara Kombucha during Microbial Composition-Controlled Fermentation

Multi-Strain Probiotics Enhance the Bioactivity of Cascara Kombucha during Microbial Composition-Controlled Fermentation

From Nature to Lab: Sustainable Bacterial Cellulose Production and Modification with Synthetic Biology

Production of Bacterial Exopolysaccharides: Xanthan and Bacterial Cellulose

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