A novel cluster of Mycobacterium abscessus complex revealed by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS)

Suzuki, H.; Yoshida, Shiomi; Yoshida, Atsushi; Okuzumi, Katsuko; Fukusima, Atsuhito; Hishinuma, Akira

doi:10.1016/j.diagmicrobio.2015.08.011

Cited by 15 publications

(23 citation statements)

References 32 publications

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“…This suggests that the two peaks in our data set might be ambiguous and not specific enough for identification, which might be due in part to the nature of the culture conditions used. There is no clear evidence, however, that such conditions prominently interfere with MALDI-TOF spectra (18,25).…”

Section: Discussionmentioning

confidence: 92%

“…massiliense from the other two subspecies (23)(24)(25)(26)(27). However, discrepancies in the specific peak signals of the three subspecies were reported.…”

Section: Discussionmentioning

confidence: 93%

“…massiliense (24). These hybrid allelic forms and associated proteomes displayed by several M. abscessus isolates were reported in previous studies (11,24,25,44). In addition, misidentification of the M. abscessus subsp.…”

Section: Discussionmentioning

confidence: 99%

“…However, improvements in MALDI-TOF MS are needed to identify very close taxa that include the M. abscessus subspecies (23). A number of studies used spectrum analysis to show that several peaks differentiated M. abscessus at the subspe-cies level; these findings, however, were inconsistent (23)(24)(25)(26)(27). Possibly, these inconsistencies in subspecies profiles were determined by geographic location and related to the horizontal transfer of genes during the evolution of M. abscessus worldwide.…”

mentioning

confidence: 81%

“…However, discrepancies in the specific peak signals of the three subspecies were reported. (25,26). Peaks around 7,638 m/z or 7,668 m/z, described by Taiwanese and Japanese investigators, were not included in our SuperSpectra (26,27).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry for Identification of Mycobacterium abscessus Subspecies According to Whole-Genome Sequencing

Luo

Liu

et al. 2016

J Clin Microbiol

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cThis study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry with the Vitek MS Plus system for identifying Mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. A total of 175 clinical M. abscessus strains were identified by whole-genome sequencing analysis: 139 Mycobacterium abscessus subsp. abscessus and 36 Mycobacterium abscessus subsp. massiliense. The research-use-only (RUO) Saramis Knowledge Base database v.4.12 was modified accordingly by adding 40 M. abscessus subsp. abscessus and 19 M. abscessus subsp. massiliense reference spectra to construct subspecies SuperSpectra. A blind test, used to validate the remaining 116 isolates, yielded 99.1% (n ‫؍‬ 115) reliability and only 0.9% (n ‫؍‬ 1) error for subspecies identification. Among the two subspecies SuperSpectra, two specific peaks were found for M. abscessus subsp. abscessus and four specific peaks were found for M. abscessus subsp. massiliense. Our study is the first to report differential peaks 3,354.4 m/z and 6,711.1 m/z, which were specific for M. abscessus subsp. massiliense. Our research demonstrates the capacity of the Vitek MS RUO Saramis Knowledge Base database to identify M. abscessus at the subspecies level. Moreover, it validates the potential ease and accuracy with which it can be incorporated into the IVD system for the identification of M. abscessus subspecies. Mycobacterium abscessus is one of the most common pathogens isolated from patients with cystic fibrosis and the second most prevalent, rapidly growing nontuberculous mycobacteria (NTM) species causing NTM pulmonary diseases (1, 2). Although it might not be the most virulent NTM pathogen, inherent antibiotic resistance makes it notoriously difficult to treat (3-7). Among multiple resistance mechanisms, resistance to macrolides is noteworthy (8). In this regard, acquired resistance to clarithromycin is always associated with a mutation in the 23S rRNA gene; intrinsic resistance is conferred mainly by the erythromycin methylase (erm) gene (3). The erm gene plays a significant role in the drug susceptibility and antibiotic resistance of M. abscessus. Mycobacterium abscessus is divided into three subspecies: Mycobacterium abscessus subsp. massiliense, Mycobacterium abscessus subsp. bolletii, and Mycobacterium. abscessus subsp. abscessus (9). The most meaningful genomic difference between the three subspecies is that M. abscessus subsp. massiliense possesses an incomplete and inactive erm gene, which is 276 bp in length and harbors deletion mutations at two positions (10-13). M. abscessus subsp. massiliense can acquire clarithromycin resistance as a consequence of a 23S rRNA mutation at position A2058 (12,14). Due to an inactive erm gene, M. abscessus subsp. massiliense does not exhibit inducible resistance after exposure to macrolides. M. abscessus subsp. abscessus and M. abscessus subsp. bolletii, on the other hand, exhibit inducible resistance (9, 15). As a result, lung diseas...

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Section: Discussionmentioning

confidence: 92%

“…massiliense from the other two subspecies (23)(24)(25)(26)(27). However, discrepancies in the specific peak signals of the three subspecies were reported.…”

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry for Identification of Mycobacterium abscessus Subspecies According to Whole-Genome Sequencing

Luo

Liu

et al. 2016

J Clin Microbiol

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Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

Opota

Prod’hom

Greub

2017

MALDI‐TOF and Tandem MS for Clinical Microbiology

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Until recently, microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification.

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Mycobacterium abscessus Complex Infections in Children: A Review

Sabin

Ferrieri

Kline

2017

Curr Infect Dis Rep

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Purpose of Review Infections in children with Mycobacterium abscessus complex represent a particular challenge for clinicians. Increasing incidence of these infections worldwide has necessitated focused attention to improve both diagnostic as well as treatment modalities. Published medical literature was reviewed, with emphasis on material published in the past 5 years. Recent Findings Increasing availability of new diagnostic tools, such as matrix-assisted laser desorption ionization-time of flight mass spectrometry and custom PCRs, has provided unique insights into the subspecies within the complex and improved diagnostic certainty. Microbiological review of all recent isolates at the University of Minnesota Medical Center was also conducted, with description of the antimicrobial sensitivity patterns encountered in our center, and compared with those published from other centers in the recent literature. A discussion of conventional antimicrobial treatment regimens, alongside detailed description of the relevant antimicrobials, is derived from recent publications. Summary Antimicrobial therapy, combined with surgical intervention in some cases, remains the mainstay of pediatric care. Ongoing questions remain regarding the transmission mechanics, immunologic vulnerabilities exploited by these organisms in the host, and the optimal antimicrobial regimens necessary to enable a reliable cure. Updated treatment guidelines based on focused clinical studies in children and accounting especially for the immunocompromised children at greatest risk are very much needed.

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A novel cluster of Mycobacterium abscessus complex revealed by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS)

Cited by 15 publications

References 32 publications

Evaluation of Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry for Identification of Mycobacterium abscessus Subspecies According to Whole-Genome Sequencing

Evaluation of Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry for Identification of Mycobacterium abscessus Subspecies According to Whole-Genome Sequencing

Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

Mycobacterium abscessus Complex Infections in Children: A Review

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