A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

Abstract

Mesenchymal Stromal Cells (MSCs) are tissue homeostatic and immune modulatory cells that have shown beneficial effects in kidney diseases and transplantation. Perivascular Stromal Cells (PSCs) share characteristics with bone marrow MSCs (bmMSCs). However, they also possess, most likely due to local imprinting, tissue-specific properties and play a role in local tissue homeostasis. This tissue specificity may result in tissue specific repair, also within the human kidney. We previously showed that human kidney PSCs (kPSCs) have enhanced kidney epithelial wound healing whereas bmMSCs did not have this potential. Moreover, kPSCs can ameliorate kidney injury in vivo. Therefore, kPSCs constitute an interesting source for cell therapy, particularly for kidney diseases and renal transplantation. Here we show the detailed isolation and culture method for kPSCs from transplant-grade human kidneys based on whole-organ perfusion of digestive enzymes via the renal artery and enrichment for the perivascular marker NG2. In this way, large cell quantities can be obtained that are suitable for cellular therapy.

Mesenchymal stem cell-based bone tissue engineering for veterinary practice

“…Hence, the application of mesenchymal stem cells (MSCs) has been investigated for bone tissue engineering utilization (Amini et al., 2012). Isolations of MSCs from various tissue resources have been reported for example bone marrow (Bianco et al., 2001; Macrin et al., 2017), adipose tissues (Zuk et al., 2002), dental tissues (Miura et al., 2003; Shi and Gronthos, 2003), umbilical cord blood (Rosada et al., 2003), peripheral blood (Kuznetsov et al., 2001), amniotic fluid (In 't Anker et al., 2004), brain (Crisan et al., 2008; Lojewski et al., 2015), skin (Joannides et al., 2004), heart (de Paula et al., 2017), kidney (Leuning et al., 2017a, b), and liver (El-Kehdy et al., 2016). In this review, potential application of three main MSCs in veterinary bone tissue engineering namely a) bone marrow-derived MSCs (BM-MSCs), b) adipose-derived MSCs (AD-MSCs), and c) dental tissue-derived MSCs, is discussed.…”