A novel CHH gene from the Pacific white shrimp Litopenaeus vannamei was characterized and found highly expressed in gut and less in eyestalk and other extra-eyestalk tissues

Ventura-López, Claudia; Goméz‐Anduro, Gracia; Arcos, Fabiola G.; Llera-Herrera, Raúl; Racotta, Ilie S.; Ibarra, Ana M.

doi:10.1016/j.gene.2016.02.011

Cited by 27 publications

(25 citation statements)

References 77 publications

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“…Secondly, as shown here and elsewhere (e.g. Hsu et al, 2006;Ventura-López et al, 2016) some of these genes are differentially expressed. Thus, if a gene is predominantly expressed in a tissue not included in the analysis, it may not be found.…”

Section: Chh and Mihsupporting

confidence: 68%

Peer Review #2 of "Similarities between decapod and insect neuropeptidomes (v0.1)"

Ye¹

2016

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Background. Neuropeptides are important regulators of physiological processes and behavior. Although they tend to be generally well conserved, recent results using trancriptome sequencing on decapod crustaceans give the impression of significant differences between species, raising the question whether such differences are real or artefacts. Methods. The BLAST+ program was used to find short reads coding neuropeptides and neurohormons in publicly available short read archives. Such reads were then used to find similar reads in the same archives and the DNA assembly program Trinity was employed to construct contigs encoding the neuropeptide precursors as completely as possible. Results. The seven decapod species analyzed in this fashion, the crabs Eriocheir sinensis, Carcinus maenas and Scylla paramamosain, the shrimp Litopenaeus vannamei, the lobster Homarus americanus, the fresh water prawn Macrobrachium rosenbergii and the crayfish Procambarus clarkii had remarkably similar neuropeptidomes. Although some neuropeptide precursors could not be assembled, in many cases individual reads pertaining to the missing precursors show unambiguously that these neuropeptides are present in these species. In other cases the tissues that express those neuropeptides were not used in the construction of the cDNA libraries. One novel neuropeptide was identified, elongated PDH (pigment dispersing hormone), a variation on PDH that has a two amino acid insertion in its core sequence. Hyrg is another peptide that is ubiquitously present in decapods and is likely a novel neuropeptide precursor. Discussion. Many insect species have lost one or more neuropeptide genes, but apart from elongated PDH and hyrg all other decapod neuropeptides are present in at least some insect species and allatotropin is the only insect neuropeptide missing from decapods. This strong similarity between insect and decapod neuropeptidomes makes it possible to predict the receptors for decapod neuropeptides that have been deorphanized in insects. This includes the androgenic insulin-like peptide that seems to be homologous to drosophila insulin-like peptide 8.

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Section: Chh and Mihsupporting

confidence: 68%

Peer Review #2 of "Similarities between decapod and insect neuropeptidomes (v0.1)"

Ye¹

2016

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“…Alignment of the CHH mature peptide sequences of L. vannamei . GenBank accession numbers: CHH‐A (AAR11295.1) (Lago‐Lestón et al., 2007); LV‐CHH (ACT76341), but without the 45 amino acids of the intron that were translated and included in the peptide (Wanlem et al., 2011), LvCHH (ADL27417) (Liu et al ., 2014), MIH‐like (AAC60516) (Sun, 1994), CHH‐B1 (AAN86056.1) (Camacho‐Jiménez et al., 2015; Lago‐Lestón et al., 2007), LvITP (ABN11282) (Tiu, He, & Chan, 2007), CHH‐B2 (AAN86057.1) (Camacho‐Jiménez, Sánchez‐Castrejón, et al, 2017; Lago‐Lestón et al., 2007) and LvCHH_PO_EF (AJK31205.1) (Ventura‐López et al, 2016) [Colour figure can be viewed at wileyonlinelibrary.com]…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Figure 6, the L. vannamei CHH peptides are highly conserved in the first 40 amino acids of the N‐terminal sequence, except for LvCHH_PO_ES (GenBank accession No. AJK31205.1) that was classified as a type‐I CHH family member but had characteristics of the type‐II CHH family (Ventura‐López et al, 2016). The authors obtained this sequence from the annotation results of the contigs of sequenced SSH libraries from juvenile females’ eyestalk and from adult female gonads that had pericardial tissue attached to it.…”

Section: Resultsmentioning

confidence: 99%

Effect of recombinant crustacean hyperglycemic hormones rCHH‐B1 and rCHH‐B2 on lipid metabolism in the Pacific white shrimp Litopenaeus vannamei

et al. 2020

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The crustacean hyperglycemic hormones (CHH) are pleiotropic neuropeptides controlling diverse processes in the life cycle of crustaceans. In the Pacific white shrimp Litopenaeus vannamei, CHH‐B1 and CHH‐B2 are generated by alternative splicing of the same gene, and the recombinant versions of these peptides have shown differential effects on glucose metabolism and osmoregulation in this species. In this work, we explored the role of both recombinant variants, expressed in yeast Pichia pastoris, on lipid metabolism. The results showed that rCHH‐B1 and rCHH‐B2 injections into eyestalk‐ablated shrimp significantly increased the phospholipid and triglyceride levels in haemolymph and restored free fatty acid levels, suggesting that these CHH variants mobilize lipid reserves in shrimp. The effects on lipids were concomitant to its hyperglycemic activity, suggesting that both variants, in addition to their hyperglycemic activity, have hyperlipidemic functions.

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“…A recent report characterized a novel CHH transcript and gene in the shrimp Litopenaeus vannamei that was identified as a type I CHH and found to be expressed in other organs in this shrimp [33]. CHH has also been shown to be present in granular cells and hemocytes [34].…”

Section: Resultsmentioning

confidence: 99%

Crustacean hyperglycemic hormone is synthesized in the eyestalk and brain of the crayfish Procambarus clarkii

2017

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Crustacean hyperglycemic hormone (CHH) is a neuropeptide that is synthesized, stored, and released by brain and eyestalk structures in decapods. CHH participates in the regulation of several mechanisms, including increasing the level of glucose in hemolymph. Although CHH mRNA levels have been quantified and the CHH protein has been localized in various structures of the crayfish P. clarkii, CHH synthesis has only been reported in the X-organ-sinus gland (XO-SG). Therefore, the aim of this study was to use in situ hybridization to determine whether CHH mRNA is located in other structures, including the putative pacemaker, eyestalk and brain, of crayfish P. clarkii at two times of day. CHH mRNA was observed in both the eyestalk and the brain of P. clarkii, indicating that CHH is synthesized in several structures in common with other crustaceans, possibly to provide metabolic support for these regions by increasing glucose levels.

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A novel CHH gene from the Pacific white shrimp Litopenaeus vannamei was characterized and found highly expressed in gut and less in eyestalk and other extra-eyestalk tissues

Cited by 27 publications

References 77 publications

Peer Review #2 of "Similarities between decapod and insect neuropeptidomes (v0.1)"

Peer Review #2 of "Similarities between decapod and insect neuropeptidomes (v0.1)"

Effect of recombinant crustacean hyperglycemic hormones rCHH‐B1 and rCHH‐B2 on lipid metabolism in the Pacific white shrimp Litopenaeus vannamei

Crustacean hyperglycemic hormone is synthesized in the eyestalk and brain of the crayfish Procambarus clarkii

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