A novel, cell‐permeable, collagen‐based membrane promotes fibroblast migration

Sadeghi, Rokhsareh; Mahdavi, Peiman; Lee, W. S.; Quan, Bryan D.; Sone, Eli D.; Ganß, Bernhard; McCulloch, Christopher A.

doi:10.1111/jre.12557

Cited by 6 publications

(10 citation statements)

References 47 publications

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“…The application of CPC in concert with collagen membranes leads to enhanced alveolar bone growth compared with controls, indicating that the inclusion of CPC with collagen membranes may simplify wound closure. We also found a reduction of pocket depth (p < 0.05) due to the inclusion of IGF-1 in the membranes, which is consistent with a previous in vitro study that described the inclusion of IGF-1 in collagen membranes and the effect of this cytokine in promoting cell migration (Sadeghi et al, 2018). Our use of Yorkshire pigs as a large animal model for screening of different treatments indicates that pigs may be used in early stage studies to examine promotion of reattachment of periodontal tissues to the denuded root surface and in the restoration of lost alveolar bone.…”

Section: Discussionsupporting

confidence: 92%

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Screening of functionalized collagen membranes with a porcine periodontal regeneration model

et al. 2022

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ObjectivesCurrent methods for periodontal regeneration do not promote collagen fiber insertions into new bone and cementum. We used a pig wound model to screen different functionalized collagen membranes in promoting periodontal reattachment to root surfaces.MethodsTreatment groups included (1) control with no membranes, (2) collagen‐coated membranes, (3) membranes with insulin‐like growth factor‐1 (IGF‐1), (4) membranes with amelotin, or (5) membranes attached with calcium phosphate cement (CPC), or with CPC combined with IGF‐1. Flap procedures were performed on mandibular and maxillary premolars of each pig.ResultsHistomorphometric, micro‐CT, and clinical measurements obtained at 4 and 12 weeks after surgery showed cementum formation on denuded roots and reformation of alveolar bone, indicating that the pig model can model healing responses in periodontal regeneration. Calcium phosphate cement simplified procedures by eliminating the need for sutures and improved regeneration of alveolar bone (p < 0.05) compared with other treatments. There was a reduction (p < 0.05) of PD only for the IGF group. Large observed variances between treatment groups indicated that a priori power analyses should be conducted to optimize statistical analysis.ConclusionsPigs can model discrete elements of periodontal healing using collagen‐based, functionalized membranes. Screening indicates that membrane anchorage with calcium phosphate cements improve regeneration of alveolar bone.

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Section: Discussionsupporting

confidence: 92%

“…Membranes for implantation into periodontal tissues were prepared in a similar manner as described (Sadeghi et al, 2018). Briefly, 3 mg/ mL porcine atelo collagen type I (Felxicol, Advanced Biomatrix, Carlsbad, CA) was combined with 10 μg/mL FN (final concentration; Sigma) or rh-AMTN (final concentration 530 μg/mL).…”

Section: Preparation Of Membranesmentioning

confidence: 99%

Screening of functionalized collagen membranes with a porcine periodontal regeneration model

et al. 2022

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“…The average cell migration index is small for all the study groups. This is not specific to CNT/GC hydrogel, as it has been previously reported that in normal in vitro conditions only a few percents of fibroblasts migrate through chemotactic mechanisms 40,43 . The cell concentration that we used in the chemoattractant-induced cell migration experiment was less than 7 million cells/ml, which is drastically lower than that of a native vocal fold tissue (66~300 million cells/ml) 44,45 .…”

Section: Discussionmentioning

confidence: 76%

“…The fibroblasts were passed, counted, and encapsulated in vials of hydrogels. Since the overall migration rate was predicted to be small 40 , we aimed for an initial population of 1~1.4 million fibroblasts in each insert to obtain enough migrated cells for comparison. Prior to gelation, 200 μl of the hydrogel was transferred from the vials to porous www.nature.com/scientificreports www.nature.com/scientificreports/ inserts.…”

Section: Chemoattractant-induced Cell Migration Cell Migration In Comentioning

confidence: 99%

Carbon nanotubes promote cell migration in hydrogels

Ravanbakhsh

Bao

Mongeau

2020

Sci Rep

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injectable hydrogels are increasingly used for in situ tissue regeneration and wound healing. ideally, an injectable implant should promote the recruitment of cells from the surrounding native tissue and allow cells to migrate freely as they generate a new extracellular matrix network. nanocomposite hydrogels such as carbon nanotube (cnt)-loaded hydrogels have been hypothesized to promote cell recruitment and cell migration relative to unloaded ones. to investigate this, cnt-glycol chitosan hydrogels were synthesized and studied. Chemoattractant-induced cell migration was studied using a modified Boyden Chamber experiment. Migrated cells were counted using flow cytometry. Cell adhesion was inferred from the morphology of the cells via an image segmentation method. cell migration and recruitment results confirmed that small concentrations of CNT significantly increase cell migration in hydrogels, thereby accelerating tissue regeneration and wound healing in situations where there is insufficient migration in the unloaded matrix. Hydrogels offer many advantages for biomedical applications due to their unique properties, in particular, their biocompatibility, biodegradation rate, mechanical stiffness, hydrophilicity, and porosity 1,2. Nanocomposite hydrogels have the potential to further improve upon these properties through the addition of nanoparticles to pure hydrogels. Such nanocomposite hydrogels have been reported to increase three-and four-dimensional printability, stiffness tunability, and electrical conductivity 3-9. For tissue engineering applications, composite hydrogels should allow sufficient cell adhesion and cell migration within its network. Such cell-biomaterial interaction is known to accelerate wound healing and tissue regeneration in the target region 10,11. Collagen, fibrin, fibronectin, nanoclays, cellulose nanocrystals, cellulose nanowhiskers, graphene oxide, and carbon nanotubes (CNTs) are among the many functional reinforcements available for fabricating bionanocomposite hydrogels 7,12-16. In our previous work, we found that the addition of CNTs to a glycol-chitosan matrix alters gelation time, porosity, and storage modulus 17. Since their discovery in 1991 by Iijima 18 , CNTs have been broadly used in biomedical applications. The CNTs can firmly attach to proteins and receptors on the cell membrane. The adherence between CNTs and cells is important. Firstly, single-walled carbon nanotubes (SWCNTs) may be employed for the purpose of drug delivery 19. Gangrade et al. took advantage of the thermal properties of the SWCNTs to obtain a temperature-dependent release of preloaded doxorubicin 20. Secondly, multiwalled carbon nanotubes (MWCNTs), which due to their size are more practical for tissue engineering applications, can be used as focal adhesion sites to enhance cell adhesion for anchorage-dependent cells such as fibroblasts 21,22. The latter may lead to an increased cell migration rate in CNT-loaded composite hydrogels. The larger diameter of MWCNTs with respect to SWCNTs approximates the p...

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“…Despite this, there were numerous other studies that employed even more complex collagen matrices than a simple coating, confirming the positive effect of this structural protein on the growth, vitality, and migration of fibroblasts [ 51 , 52 , 53 , 54 , 55 , 56 ].…”

Section: Discussionmentioning

confidence: 96%

Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study

Romasco

Mandrillo²,

Morsut

et al. 2023

Biomedicines

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Maintaining periodontal and peri-implant soft tissues health is crucial for the long-term health of teeth and dental implants. New biomedical strategies aimed at avoiding connective tissue alterations and related diseases (e.g., periodontitis and peri-implantitis) are constantly evolving. Among these, collagen-based medical products have proven to be safe and effective. Accordingly, the aim of the present study was to evaluate the effects of Dental SKIN BioRegulation (Guna S.p.a., Milan, Italy), a new injectable medical device composed of type I collagen of porcine origin, on primary cultures of human gingival fibroblasts (hGF). To this end, hGF were cultured on collagen-coated (COL, 100 µg/2 mL) or uncoated plates (CTRL) before evaluating cell viability (24 h, 48 h, 72 h, and 7 d), wound healing properties (3 h, 6 h, 12 h, 24 h, and 48 h), and the activation of mechanotransduction markers, such as FAK, YAP, and TAZ (48 h). The results proved a significant increase in cell viability at 48 h (p < 0.05) and wound closure at 24 h (p < 0.001) of hGF grown on COL, with an increasing trend at all time-points. Furthermore, COL significantly induced the expression of FAK and YAP/TAZ (p < 0.05), thereby promoting the activation of mechanotransduction signaling pathways. Overall, these data suggest that COL, acting as a mechanical bio-scaffold, could represent a useful treatment for gingival rejuvenation and may possibly help in the resolution of oral pathologies.

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A novel, cell‐permeable, collagen‐based membrane promotes fibroblast migration

Abstract: Incorporation of FN into IGF-I-loaded collagen+Vicryl membranes reduced IGF release from collagen and increased the migration of human gingival fibroblasts. The new membrane may promote healing and reformation of the dentogingival junction.

Cited by 6 publications

References 47 publications

Screening of functionalized collagen membranes with a porcine periodontal regeneration model

Screening of functionalized collagen membranes with a porcine periodontal regeneration model

Carbon nanotubes promote cell migration in hydrogels

Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study

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