A novel cartilage tissue construction based on artificial cells and matrix-shaping

Liu, Yuangang; Tang, Xiangwei; Wang, Shibin; Chen, Ai-Zheng; Wu, Wenguo; Long, Ruimin

doi:10.1016/j.matlet.2015.06.068

Cited by 3 publications

(6 citation statements)

References 21 publications

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“…In the study of Zhang (Liu et al, 2015) (Zhang et al, 2006, polylysine was used as a membrane material, and…”

Section: Methodsmentioning

confidence: 99%

“…The cell suspension was adjusted to 1 × 10 4 /ml and dropped into 1.5% (w/v) calcium chloride solution through a high-voltage electrostatic droplet generator (parameter setting: 7.5 kV, 20 mm/h) to form Alg beads embedded with cells. The obtained beads were coated with a filter-sterilized chitosan solution for 8 min to obtain alginate/chitosan microcapsules, which were then suspended in 0.15% (w/v) Alg solution to form ACA microcapsules ( Gomes et al, 2022 ) ( Liu et al, 2015 ), and the core was liquefied with 1.6% (w/v) sodium citrate. The collected microcapsules were washed with saline, and placed in an incubator containing 5% CO2 at 37°C.…”

Section: Methodsmentioning

confidence: 99%

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3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs

Long

Shi²,

He³

et al. 2022

Front. Bioeng. Biotechnol.

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The use of hydrogel as a filling medium to recombine dispersed microencapsulated cells to form an embedded gel-cell microcapsule complex is a new idea based on bottom-up tissue construction, which is benefit for cell distribution and of great significance for tissue construction research in vitro. In this experiment, sodium alginate and chitosan were used as the main materials, rat normal liver cell BRL-3A was used as the model cell to prepare “artificial cells”. Silkworm pupa was used as raw material to extract silk fibroin solution, which was prepared by ultrasound to be the silk fibroin gel; silk fibroin hydrogel-microencapsulated hepatocyte embedded complex was then prepared by using silk fibroin gel as filling medium; the complex was cultured under three modes (static, shaking, and 3D microgravity), and the tissue forming ability of rat hepatocytes was investigated. The results showed that the microgravity culture condition can enhance the cell proliferation and promote the formation of cell colonies in the microcapsules; silk fibroin can form an embedded gel-cell microcapsule complex with microencapsulated cells, which provided mechanical support for the structure of the composite. We hope that this bottom-up construction system will have potential applications in the fields of cell culture and tissue construction.

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“…In the study of Zhang (Liu et al, 2015) (Zhang et al, 2006, polylysine was used as a membrane material, and…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs

Long

Shi²,

He³

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

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“…The single cell suspension was firstly added into 2 mL of 1.5% alginate solution, which was further dropped into 1.5% CaCl 2 solution by high-voltage electrostatic method (voltage: 5.5 kV; needle: 7 # ; distance of needle and CaCl 2 solution: 2 cm; propulsion speed: 50 mm/h) to obtain alginate-calcium (Alg-Ca) beads loaded with C5.18 cells. In order to form alginate/chitosan (AC) film, C5.18 cell-loaded Alg-Ca beads were made up to 0.5% chitosan solution (pH 5.5) for a 10-min film reaction after being filtered by tea leakage and the beads with the chitosan (CS) film were transferred to sodium alginate solution to form an AC film [10]. Next, the liquefied core of the artificial cells was prepared with 1.6% sodium citrate solution for 2 min after being washed by saline.…”

Section: Methodsmentioning

confidence: 99%

“…For example, cells are sometimes difficult to grow into scaffolds and present uneven distribution. Different culture conditions can cause different viability in cells and further present different results in tissue engineering, which Liu has reported before [10]. Compared to 2D conditions, 3D cultures can protect chondrocyte models from species-specific acute drug toxicity in vitro and can also maintain cell phenotype.…”

Section: Introductionmentioning

confidence: 99%

The Influence of bFGF on the Fabrication of Microencapsulated Cartilage Cells under Different Shaking Modes

et al. 2019

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Cell encapsulation in hydrogels has been extensively used in cytotherapy, regenerative medicine, 3D cell culture, and tissue engineering. Herein, we fabricated microencapsulated cells through microcapsules loaded with C5.18 chondrocytes alginate/chitosan prepared by a high-voltage electrostatic method. Under optimized conditions, microencapsulated cells presented uniform size distribution, good sphericity, and a smooth surface with different cell densities. The particle size distribution was determined at 150–280 μm, with an average particle diameter of 220 μm. The microencapsulated cells were cultured under static, shaking, and 3D micro-gravity conditions with or without bFGF (basic fibroblast growth factor) treatment. The quantified detection (cell proliferation detection and glycosaminoglycan (GAG)/type II collagen (Col-II)) content was respectively determined by cell counting kit-8 assay (CCK-8) and dimethylmethylene blue (DMB)/Col-II secretion determination) and qualitative detection (acridine orange/ethidium bromide, hematoxylin-eosin, alcian blue, safranin-O, and immunohistochemistry staining) of these microencapsulated cells were evaluated. Results showed that microencapsulated C5.18 cells under three-dimensional microgravity conditions promoted cells to form large cell aggregates within 20 days by using bFGF, which provided the possibility for cartilage tissue constructs in vitro. It could be found from the cell viability (cell proliferation) and synthesis (content of GAG and Col-II) results that microencapsulated cells had a better cell proliferation under 3D micro-gravity conditions using bFGF than under 2D conditions (including static and shaking conditions). We anticipate that these results will be a benefit for the design and construction of cartilage regeneration in future tissue engineering applications.

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“…The MACI method involves the development of degradable cartilage mimicking scaffolds, which are impregnated with chondrocytes [ 64 ]. For example, using in vitro methods, cells that were implanted into low melting point agarose matrixes, produced large collagen concentrations within 20 days [ 90 ]. As stated previously, the primary issue with the MACI model is that the current knowledge on these materials requires further investigation to accurately understand the rate of degradation of the degradable scaffolds.…”

Section: Synthetic Materials Used In Joint Replacement and Cartilamentioning

confidence: 99%

Hydrogels as a Replacement Material for Damaged Articular Hyaline Cartilage

et al. 2016

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Hyaline cartilage is a strong durable material that lubricates joint movement. Due to its avascular structure, cartilage has a poor self-healing ability, thus, a challenge in joint recovery. When severely damaged, cartilage may need to be replaced. However, currently we are unable to replicate the hyaline cartilage, and as such, alternative materials with considerably different properties are used. This results in undesirable side effects, including inadequate lubrication, wear debris, wear of the opposing articular cartilage, and weakening of the surrounding tissue. With the number of surgeries for cartilage repair increasing, a need for materials that can better mimic cartilage, and support the surrounding material in its typical function, is becoming evident. Here, we present a brief overview of the structure and properties of the hyaline cartilage and the current methods for cartilage repair. We then highlight some of the alternative materials under development as potential methods of repair; this is followed by an overview of the development of tough hydrogels. In particular, double network (DN) hydrogels are a promising replacement material, with continually improving physical properties. These hydrogels are coming closer to replicating the strength and toughness of the hyaline cartilage, while offering excellent lubrication. We conclude by highlighting several different methods of integrating replacement materials with the native joint to ensure stability and optimal behaviour.

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A novel cartilage tissue construction based on artificial cells and matrix-shaping

Cited by 3 publications

References 21 publications

3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs

3D cell culture based on artificial cells and hydrogel under microgravity for bottom-up microtissue constructs

The Influence of bFGF on the Fabrication of Microencapsulated Cartilage Cells under Different Shaking Modes

Hydrogels as a Replacement Material for Damaged Articular Hyaline Cartilage

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