A novel biological sources consistency evaluation method reveals high level of biodiversity within wild natural medicine: A case study of Amynthas earthworms as “Guang Dilong”

Xing, Zhimei; Gao, Han; Wang, Dan; Shang, Ye; Tuliebieke, Tenukeguli; Jiang, Jibao; Li, Chunxiao; Wang, Hong; Li, Zhenguo; Jia, Lifu; Yong-sheng, WU; Wang, Dandan; Yang, Wenzhi; Chang, Yan‐xu; Zhang, Xiaoying; Xu, Liuwei; Jiang, Chao; Huang, Luqi; Tian, Xiaoxuan

doi:10.1016/j.apsb.2022.10.024

Cited by 6 publications

(3 citation statements)

References 81 publications

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“…The strategy based on DNA metabarcoding combined with minibarcode enables precise identification of the origin of TCM. Importantly, this novel strategy can reveal distinct genetic variations in TCM that contribute to changes in chemical components and biological activity, though the morphological characteristics of the wild source medicine show no apparent differences [ 26 , 27 ].…”

Section: The Modernization Of Traditional Chinese Medicinementioning

confidence: 99%

Lab-on-a-chip: an advanced technology for the modernization of traditional Chinese medicine

Lu,

Yuan,

Han

et al. 2024

Chin Med

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Benefiting from the complex system composed of various constituents, medicament portions, species, and places of origin, traditional Chinese medicine (TCM) possesses numerous customizable and adaptable efficacies in clinical practice guided by its theories. However, these unique features are also present challenges in areas such as quality control, screening active ingredients, studying cell and organ pharmacology, and characterizing the compatibility between different Chinese medicines. Drawing inspiration from the holistic concept, an integrated strategy and pattern more aligned with TCM research emerges, necessitating the integration of novel technology into TCM modernization. The microfluidic chip serves as a powerful platform for integrating technologies in chemistry, biology, and biophysics. Microfluidics has given rise to innovative patterns like lab-on-a-chip and organoids-on-a-chip, effectively challenging the conventional research paradigms of TCM. This review provides a systematic summary of the nature and advanced utilization of microfluidic chips in TCM, focusing on quality control, active ingredient screening/separation, pharmaceutical analysis, and pharmacological/toxicological assays. Drawing on these remarkable references, the challenges, opportunities, and future trends of microfluidic chips in TCM are also comprehensively discussed, providing valuable insights into the development of TCM.

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Section: The Modernization Of Traditional Chinese Medicinementioning

confidence: 99%

Lab-on-a-chip: an advanced technology for the modernization of traditional Chinese medicine

Lu,

Yuan,

Han

et al. 2024

Chin Med

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“…However, market research showed that 44% of commercial Pheretima samples were from PA and PV, approximately 55% from Metaphire magna (MM), an unofficial species, and less than 1% from PG and PP [ 5 ]. Additionally, there are several sympatrically distributed related species with similar morphological characteristics, making it difficult to distinguish authentic medicines from adulterated species [ 6 ] as well as to identify the source of Pheretima in CPMs. Although much work has been done on the authentication of Pheretima species, such as amino acids [ 7 ], endogenous small molecules [ 1 ], tryptic peptides [ 5 , 8 ], DNA barcoding [ 9 ], and biological source consistency evaluation [ 6 ], a viable approach to identify Pheretima species in CPMs is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics

Luo

Huang

et al. 2023

Journal of Pharmaceutical Analysis

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“…In recent years, the emergence of DNA metabarcoding, a technology that combines high-throughput sequencing and DNA barcoding, has enabled the simultaneous detection of multispecies barcode sequences in mixed samples 17 . This approach has been successfully applied to the discovery of biodiversity through environmental DNA 18 , bulk DNA from multiple individuals 19 , and stool DNA from various sources 20 . In the eld of TCMs, selecting appropriate barcodes is a critical step in DNA metabarcoding of TCPM.…”

Section: Introductionmentioning

confidence: 99%

Qualitative identification of lonicerae japonicae flos in traditional chinese medicine using metabarcoding combined with specific mini-barcodes

Gao,

Zhang,

Wang

et al. 2023

Mol Biol Rep

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BACKGROUND: Lonicerae japonicae os, also known as Jinyinhua (JYH), is an important component of traditional Chinese patent medicine (TCPM) products. However, the potential for adulteration and substitution with low-quality materials highlights the need for a reliable and sensitive approach to identify the species composition of TCPM products for consumer safety. METHODS AND RESULTS:We used universal ITS2 primers to amplify TCPMs containing JYH. However, the results were inconclusive, as only one operational taxonomic unit (OTU) was identi ed as Lonicera sp., which could not be identi ed at the species level. To con rm the species identi cation of Lonicera sp. in TCPM, we developed a short mini-barcode primer based on thepsbA-trnH region, which, in combination with DNA metabarcoding technology, allowed for qualitative and quantitative analysis of arti cially mixed samples. We applied the mini-barcode to distinguish TCPMs containing JYH and demonstrated its relatively accurate quantitative ability in identifying twoLonicera species. CONCLUSIONS: Our study presents a method for qualitative and quantitative identi cation of JYH, providing a promising application of DNA metabarcoding technology in the quality control of TCPM products.

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A novel biological sources consistency evaluation method reveals high level of biodiversity within wild natural medicine: A case study of Amynthas earthworms as “Guang Dilong”

Cited by 6 publications

References 81 publications

Lab-on-a-chip: an advanced technology for the modernization of traditional Chinese medicine

Lab-on-a-chip: an advanced technology for the modernization of traditional Chinese medicine

Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics

Qualitative identification of lonicerae japonicae flos in traditional chinese medicine using metabarcoding combined with specific mini-barcodes

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