Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics

Luo, Xiaoxiao; Bi, Qirui; Huang, Dongdong; Li, Yun; Yao, Changliang; Wei, Wenlong; Li, Jiayuan; Li, Zhenwei; Zhang, Jingxian; Shen, Ji; Wang, Yurong; Guo, De‐An

doi:10.1016/j.jpha.2023.06.006

Cited by 5 publications

(2 citation statements)

References 33 publications

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“…The precipitates extracted from neuropeptides were homogenized in 4% SDS, and equal amounts of proteins were taken from each group and digested with trypsin (Promega Corporation, Madison, WI, USA) in a ratio of 100:1 at 37 °C for 18 h. The digestion was terminated by 10% TFA acidification, and the extracts were then dried down in a SpeedVac vacuum concentrator to obtain a crude protein sample. The crude peptide sample and crude protein sample were further desalinated through C18 ZipTip and resuspended in 20 μL of 0.1% formic acid aqueous solution for LC-MS/MS analysis [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Pinctada martensii Hydrolysate Modulates the Brain Neuropeptidome and Proteome in Diabetic (db/db) Mice via the Gut–Brain Axis

Li,

Lv,

Wei

et al. 2024

Marine Drugs

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Pinctada martensii hydrolysate (PMH) has been proved to have the effect of ameliorating disorders of glucose and lipid metabolism in db/db mice, but the mechanism of its hyperglycemia effect is still unclear. Bacterial communities in fecal samples from a normal control group, a diabetic control group, and a PMH-treated diabetes mellitus type 2 (T2DM) group were analyzed by 16S gene sequencing. Nano LC-MS/MS was used to analyze mice neuropeptides and proteomes. The 16S rDNA sequencing results showed that PMH modulated the structure and composition of the gut microbiota and improved the structure and composition of Firmicutes and Bacteroidetes at the phylum level and Desulfovibrionaceae and Erysipelatoclostridiaceae at the family level. Furthermore, the expressions of functional proteins of the central nervous system, immune response-related protein, and proteins related to fatty acid oxidation in the brain disrupted by an abnormal diet were recovered by PMH. PMH regulates the brain neuropeptidome and proteome and further regulates blood glucose in diabetic mice through the gut–brain axis. PMH may be used as a prebiotic agent to attenuate T2DM, and target-specific microbial species may have unique therapeutic promise for metabolic diseases.

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Section: Methodsmentioning

confidence: 99%

Pinctada martensii Hydrolysate Modulates the Brain Neuropeptidome and Proteome in Diabetic (db/db) Mice via the Gut–Brain Axis

Li,

Lv,

Wei

et al. 2024

Marine Drugs

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“…Peptidomics is a method for analyzing the composition and characteristics of endogenous peptides in biological tissues or cells, typically achieved through liquid chromatography (LC) separation combined with mass spectrometry (MS) detection (Hellinger et al, 2023). The utilization of peptidomics has been widespread in identifying various peptides found in animal-derived TCMs, such as Pheretima (Luo et al, 2023), Bubali Cornu (Liu et al, 2017), and leech (Liao et al, 2023). In addition, in the drug development phase, virtual screening serves as an efficient and convenient computational simulation method.…”

mentioning

confidence: 99%

Evaluation of quality consistency between dispensing granules and traditional decoction of Bombyx batryticatus based on peptidomics and in silico simulations

Huang,

Liu,

Xiao

et al. 2024

Biomedical Chromatography

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The application of traditional Chinese medicine dispensing granules is becoming increasingly prevalent. However, the consistency of dispensing granules with traditional decoction remains controversial. In this study, the consistency of peptide composition and pharmacodynamics between dispensing granules and traditional decoction of Bombyx batryticatus (BB) were assessed. A peptidomics method based on LC–tandem mass spectrometry technology was used to evaluate peptide composition similarity between BB traditional decoction and dispensing granules. The results revealed notable differences in peptide sequences between the two dosage forms, with only 8.55% of peptides shared between them. To evaluate the potential pharmacodynamic effects of the two dosage forms on epilepsy, virtual screening was used to identify potential active peptides, including blood–brain barrier permeability, toxicity prediction, and molecular docking. BB traditional decoction demonstrated a higher number and greater abundance of potential active peptides than BB dispensing granules, suggesting that BB traditional decoction may have a more favorable effect in treating epilepsy compared with BB dispensing granules. Moreover, molecular docking and molecular dynamics simulation studies confirmed the mechanism of action of active peptides to γ‐aminobutyric acid transporter 1 (GAT‐1). This study provides a scientific basis for the evaluation of quality consistency between BB traditional decoction and dispensing granules.

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Thiols-rich peptide from water buffalo horn keratin alleviates oxidative stress and inflammation through co-regulating Nrf2/Hmox-1 and NF-κB signaling pathway

Wu,

Tang,

Bao

et al. 2024

Free Radical Biology and Medicine

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Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics

Cited by 5 publications

References 33 publications

Pinctada martensii Hydrolysate Modulates the Brain Neuropeptidome and Proteome in Diabetic (db/db) Mice via the Gut–Brain Axis

Pinctada martensii Hydrolysate Modulates the Brain Neuropeptidome and Proteome in Diabetic (db/db) Mice via the Gut–Brain Axis

Evaluation of quality consistency between dispensing granules and traditional decoction of Bombyx batryticatus based on peptidomics and in silico simulations

Thiols-rich peptide from water buffalo horn keratin alleviates oxidative stress and inflammation through co-regulating Nrf2/Hmox-1 and NF-κB signaling pathway

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