A Novel Biodegradable Hollow Nanocarrier Consisting Superparamagnetic Fe₃O₄-loaded poly-γ- glutamic Acid and Chitosan Oligosaccharide for Targeted Delivery of Sulforaphane from Broccoli Seed Extracts

Abstract

The aim of this study was to synthesize a polymeric nanocarrier system loaded with superparamagnetic iron oxide nanoparticles (SPION) and the sulforaphane(SFN) through a cross-linking method in order to protect the SFN. According to this experiment, the drug-loading content and encapsulation efficiency were 29.97±0.08% and 0.34±0.41%, respectively. The total cumulative amount of SFN, released from the nanocarrier in vitro was 87.90% over a 72 h period. MTT assays indicated that the SPION-SFN-loaded nanocarrier exhibited a much higher in vitro antitumor efficacy than free SFN. Together, these results indicate that SPION-loaded nanocarrier exhibits good drug-loading ability and sustained-release function.

Optimization of a Microencapsulation Process Using Oil-in-Water (O/W) Emulsion to Increase Thermal Stability of Sulforaphane

“…In the present work, SFN was dissolved in the organic phase, which partly explains the higher EE attained. Other works developed SFN nanoparticles and micelles using mineralized hyaluronic acid-Stetradecyl nanocarriers [26], polymeric nanocarriers loaded with superparamagnetic iron oxide nanoparticles [27], nanostructured lipid carriers [17], and prolamin-based composite nanoparticles [28]. Nanoencapsulation and micelles methods resulted in EE up to 93%, but they are not likely to be used in the food industry because they represent a significantly higher production cost in comparison with microencapsulation methods.…”