A novel biocompatible hyaluronic acid–chitosan hybrid hydrogel for osteoarthrosis therapy

Kaderli, S.; Boulocher, C.; Pillet, Elodie; Watrelot-Virieux, D.; Rougemont, Anne‐Laure; Roger, Thierry; Viguier, É.; Gurny, Robert; Scapozza, Léonardo; Jordan, Olivier

doi:10.1016/j.ijpharm.2015.01.052

Cited by 64 publications

(42 citation statements)

References 49 publications

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“…CS when combined with glycerol phosphate and HA can undergo a sol-gel transition at 37°C by covalent crosslinking. 22 We have shown in this study that increasing the HA concentration in the CS/HA thermo-sensitive hydrogel increases the gelation time, which may be attributable to the presence of more hydrophilic groups among HA chains. In addition to gel thermosensitivity, other important requirements include high water content, biodegradability as well as mechanical properties, 23 which are important to the mechanical function of the disc after implantation, and it is important that the matrix secreted from implanted cells is able to replace the hydrogel over time after the implantation.…”

Section: Discussionmentioning

confidence: 99%

Development of kartogenin-conjugated chitosan–hyaluronic acid hydrogel for nucleus pulposus regeneration

et al. 2017

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Injectable constructs for in vivo gelation have many advantages in the regeneration of degenerated nucleus pulposus. In this study, an injectable hydrogel consisting of chitosan (CS) and hyaluronic acid (HA) crosslinked with glycerol phosphate (GP) at different proportions (CS : GP : HA, 6 : 3 : 1, 5 : 3 : 2, 4 : 3 : 3, 3 : 3 : 4, 2 : 3 : 5, 1 : 3 : 6, V : V : V) was developed and employed as a delivery system for kartogenin (KGN), a biocompound that can activate chondrocytes. In vitro gelation time, morphologies, swelling, weight loss, compressive modulus and cumulative release of KGN in hydrogels were studied.For biocompatibility assessments, human adipose-derived stem cells (ADSCs) were encapsulated in these hydrogels. The effects of KGN on stem cell proliferation and differentiation into nucleus pulposus-like cells were examined. The hydrogels with higher concentrations of HA showed a slightly shorter gelation time, higher water uptake, faster weight loss and faster KGN release compared to the hydrogels with lower concentrations of HA. As the KGN-conjugated hydrogel prepared with the proportions 5 : 3 : 2 displayed good mechanical properties, it was chosen as the optimal gel to promote cell proliferation and differentiation. No significant difference was seen in the expression levels of nucleus pulposus markers induced by KGN or TGF-β. Additionally, inclusion of KGN and TGF-β together did not produce a synergistic effect in inducing nucleus pulposus properties. In conclusion, we have developed a KGN-conjugated CS/HA hydrogel (5 : 3 : 2) with sustained release of KGN in hydrogel that can promote ADSC proliferation and nucleus pulposus differentiation. This kind of hydrogel may be a simple and effective candidate for the repair of degenerative NP tissue after minimally invasive surgery.

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Section: Discussionmentioning

confidence: 99%

Development of kartogenin-conjugated chitosan–hyaluronic acid hydrogel for nucleus pulposus regeneration

et al. 2017

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“…Recently, accumulating studies have demonstrated that chitosan could be used as a deliverer to deliver the drugs, genes, and proteins into the articular space to improve OA progression (Kaderli et al, 2015;Park et al, 2013;Patchornik et al, 2012;Rodríguez-Vázquez et al, 2015;Tan et al, 2009;). Chitosan could be prepared as various forms of an intraarticular injectable scaffold to crosslink with therapeutic molecules for the clinical application of OA treatment, for example, hyaluronic/chitosan hydrogels (Kaderli et al, 2015;Park et al, 2013;Patchornik et al, 2012;Tan et al, 2009), berberine or kartogenin/chitosan nanoparticles (Kang, Ko, Kim, & Im, 2014;Zhou et al, 2015), and sinomenium/chitosan microsphere (Chen et al, 2016). Chitosan could be prepared as various forms of an intraarticular injectable scaffold to crosslink with therapeutic molecules for the clinical application of OA treatment, for example, hyaluronic/chitosan hydrogels (Kaderli et al, 2015;Park et al, 2013;Patchornik et al, 2012;Tan et al, 2009), berberine or kartogenin/chitosan nanoparticles (Kang, Ko, Kim, & Im, 2014;Zhou et al, 2015), and sinomenium/chitosan microsphere (Chen et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The pH value, prepared program, and deacetylation level of chitosan could change its properties and contribute to different applications (Huang, Onyeri, Siewe, Moshfeghian, & Madihally, 2005;Patchornik, Ram, Ben Shalom, Nevo, & Robinson, 2012;Rodríguez-Vázquez, Vega-Ruiz, Ramos-Zúñiga, Saldaña-Koppel, & Quií1ones-Olvera, 2015). Moreover, some studies have attempted to use an intraarticular injection of chitosan to repair and strengthen the surface of damaged cartilage (Kaderli et al, 2015;Park, Choi, Hu, & Lee, 2013;Patchornik et al, 2012;Rodríguez-Vázquez et al, 2015;Tan, Chu, Payne, & Marra, 2009). Moreover, some studies have attempted to use an intraarticular injection of chitosan to repair and strengthen the surface of damaged cartilage (Kaderli et al, 2015;Park, Choi, Hu, & Lee, 2013;Patchornik et al, 2012;Rodríguez-Vázquez et al, 2015;Tan, Chu, Payne, & Marra, 2009).…”

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confidence: 99%

“…Chitosan is usually used in scaffold preparation for cell culture and tissue engineering, including in vitro 3D cartilage and bone reconstruction (Rodríguez-Vázquez et al, 2015). The crosslinking of chitosan with other biomaterials, such as gelatin, hyaluronate, or glycerophosphate, has also been found to further increase the availability of chitosan in the tissue engineering of cartilage (Huang et al, 2005;Kaderli et al, 2015;Park et al, 2013;Patchornik et al, 2012;Rodríguez-Vázquez et al, 2015;Tan et al, 2009). The crosslinking of chitosan with other biomaterials, such as gelatin, hyaluronate, or glycerophosphate, has also been found to further increase the availability of chitosan in the tissue engineering of cartilage (Huang et al, 2005;Kaderli et al, 2015;Park et al, 2013;Patchornik et al, 2012;Rodríguez-Vázquez et al, 2015;Tan et al, 2009).…”

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confidence: 99%

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Glucose adsorption to chitosan membranes increases proliferation of human chondrocyte via mammalian target of rapamycin complex 1 and sterol regulatory element‐binding protein‐1 signaling

Chang

Huang

Cheng

et al. 2017

Journal Cellular Physiology

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Osteoarthritis (OA) is currently still an irreversible degenerative disease of the articular cartilage. Recent, dextrose (d-glucose) intraarticular injection prolotherapy for OA patients has been reported to benefit the chondrogenic stimulation of damaged cartilage. However, the detailed mechanism of glucose's effect on cartilage repair remains unclear. Chitosan, a naturally derived polysaccharide, has recently been investigated as a surgical or dental dressing to control breeding. Therefore, in this study, glucose was adsorbed to chitosan membranes (CTS-Glc), and the study aimed to investigate whether CTS-Glc complex membranes could regulate the proliferation of human OA chondrocytes and to explore the underlying mechanism. Human OA and SW1353 chondrocytes were used in this study. The experiments involving the transfection of cells used SW1353 chondrocytes. A specific inhibitor and siRNAs were used to investigate the mechanism underlying the CTS-Glc-regulated proliferation of human chondrocytes. We found that CTS-Glc significantly increased the proliferation of both human OA and SW1353 chondrocytes comparable to glucose- or chitosan-only stimulation. The role of mammalian target of rapamycin complex 1 (mTORC1) signaling, including mTOR, raptor, and S6k proteins, has been demonstrated in the regulation of CTS-Glc-increased human chondrocyte proliferation. mTORC1 signaling increased the expression levels of maturated SREBP-1 and FASN and then induced the expressions of cell cycle regulators, that is, cyclin D, cyclin-dependent kinase-4 and -6 in human chondrocytes. This study elucidates the detailed mechanism behind the effect of CTS-Glc complex membranes in promoting chondrocyte proliferation and proposes a possible clinical application of the CTS-Glc complex in the dextrose intraarticular injection of OA prolotherapy in the future to attenuate the pain and discomfort of OA patients.

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“…Um arcabouço incorporando um complexo de quitosana-glicosaminaglicana pode reter e concentrar fatores secretados através da colonização de células [66] . A associação de AH e QUI geralmente leva à formação de um complexo de polieletrólito (PEC) [67] . PECs são preparados geralmente através da mistura de dois polímeros de cargas opostas, o que leva a auto-montagem devido ao aumento de entropia [68] .…”

Section: Aplicação De Hidrogéis a Base De áCido Hialurônico E Quitosaunclassified

Hidrogéis a base de ácido hialurônico e quitosana para engenharia de tecido cartilaginoso

Nascimento

Lombello

2016

Polímeros

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AesumoA Engenharia de Tecidos envolve o desenvolvimento de novos materiais ou dispositivos capazes de interações específicas com os tecidos biológicos, buscando a utilização de materiais biocompatíveis que devem servir como arcabouço para o crescimento de células in vitro, organizando e desenvolvendo o tecido que posteriormente será implantado no paciente. Uma variedade de arcabouços como hidrogéis poliméricos, sintéticos e naturais, têm sido investigados para a expansão de condrócitos in vitro, visando o reparo da cartilagem lesionada. Um hidrogel de interesse particular na regeneração de cartilagem é o ácido hialurónico (AH). Trata-se de um biopolímero atraente para a fabricação de arcabouços artificiais para Engenharia de Tecidos por ser biocompatível e biodegradável. A biocompatibilidade do AH deve-se ao fato de estar presente na matriz extracelular nativa, deste modo, cria-se um ambiente propício que facilita a adesão, proliferação e diferenciação celular, além da existência de sinalização celular específica, o que contribui para a regeneração do tecido. O uso de hidrogel composto de ácido hialurónico e quitosana (QUI) também tem sido investigado em aplicações de Engenharia de Tecidos de cartilagem, com resultados promissores. Baseando-se nestas informações, o objetivo este trabalho foi investigar as alternativas disponíveis para regeneração tecidual da cartilagem e conhecer mais detalhadamente as relações entre células e biomateriais. Palavras-chave: ácido hialurônico, biocompatibilidade, engenharia de tecidos, quitosana, cultura de células. AbstractTissue Engineering involves the development of new materials or devices capable of specific interactions with biological tissues, searching the use of biocompatible materials as scaffolds for cell growth in vitro, organizing and developing tissue that is subsequently implanted into the patient. A variety of scaffolds such as polymeric hydrogels, natural and synthetic, have been investigated for the expansion of chondrocytes in vitro in order to repair the damaged cartilage. A hydrogel of particular interest in cartilage regeneration is hyaluronic acid (HA). HA are attractive biopolymers for manufacturing artificial scaffolds for Tissue Engineering, it is biocompatible and biodegradable. The biocompatibility of HA is due to the fact that it is present in native extracellular matrix, thus creates an environment, which facilitates the adhesion, proliferation and differentiation, in addition to the existence of specific cell signaling, which contributes to tissue regeneration. The use of hydrogel composed of hyaluronic acid and chitosan (CHI) has also been investigated for applications in Tissue Engineering of soft tissues, like cartilage, with promising results. Based on this information, this study aims to investigate the alternatives available for cartilage tissue regeneration and meet more detail the relationships between cells and biomaterials.

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A novel biocompatible hyaluronic acid–chitosan hybrid hydrogel for osteoarthrosis therapy

Cited by 64 publications

References 49 publications

Development of kartogenin-conjugated chitosan–hyaluronic acid hydrogel for nucleus pulposus regeneration

Development of kartogenin-conjugated chitosan–hyaluronic acid hydrogel for nucleus pulposus regeneration

Glucose adsorption to chitosan membranes increases proliferation of human chondrocyte via mammalian target of rapamycin complex 1 and sterol regulatory element‐binding protein‐1 signaling

Hidrogéis a base de ácido hialurônico e quitosana para engenharia de tecido cartilaginoso

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