A novel benzimidazole-functionalized 2-D COF material: Synthesis and application as a selective solid-phase extractant for separation of uranium

Aptamer‐Functionalized Magnetic Conjugated Organic Framework for Selective Extraction of Traces of Hydroxylated Polychlorinated Biphenyls in Human Serum

“…In Figure 4Ac, the signal intensity at 1713 cm À1 in the spectrum of Fe 3 O 4 @COF-Apt-15 decreaseds harply compared to that of Fe 3 O 4 @COF-COOH-15, due to the formation of amide bonds between carboxyl groups of Fe 3 O 4 @COF-COOH-15 and amino groups of the aptamer.M oreover, the peaks at 1636 and 1577 cm À1 could be ascribed to C=Oa nd NÀHv ibrations of amide groups, respectively.I nc onclusion, FTIR analysis illustrated that synthesis of Fe 3 O 4 @COF-Apt-15w as successful. [38] To further confirm the immobilization of the aptamer on Fe 3 O 4 @COF-COOH-15, Boehm titrations werec onducted to study the contents of carboxyl groups in Fe 3 O 4 @COF-COOH-15 and Fe 3 O 4 @COF-Apt-15. After the functionalization step,t he content descended from 7.94 to 2.43 mmol g À1 ,a nd this demonstrated that the aptamer was successfully graftedo nto Fe 3 O 4 @COF-COOH-15.…”

“…[38] To further confirm the immobilization of the aptamer on Fe 3 O 4 @COF-COOH-15, Boehm titrations werec onducted to study the contents of carboxyl groups in Fe 3 O 4 @COF-COOH-15 and Fe 3 O 4 @COF-Apt-15. [38] UV/Vis absorption spectrometry was employed to confirm conjugation of the aptamer with diffractionp eaks matched well with those of magnetite( JCPDS no. [38] UV/Vis absorption spectrometry was employed to confirm conjugation of the aptamer with diffractionp eaks matched well with those of magnetite( JCPDS no.…”

“…[11] Then, as-synthesized Fe 3 O 4 particles (2.0 g) were dispersed in am ixture of ethanol (80 mL), H 2 O( 20 mL), and NH 3 ·H 2 O( 1.5 mL, 28 wt %), followed by the addition of TEOS (1.0 mL). [38] Fe 3 O 4 @COF-COOH (1.0 g) was dispersed in PBS (50 mL, pH 7.4) containing EDC (0.8 g) and NHS (1.2 g). Subsequently,t he suspension was stirred at 25 8Cf or 1hto obtain Fe 3 O 4 @SiO 2 @NH 2 .…”

“…After the functionalization step,t he content descended from 7.94 to 2.43 mmol g À1 ,a nd this demonstrated that the aptamer was successfully graftedo nto Fe 3 O 4 @COF-COOH-15. [38] UV/Vis absorption spectrometry was employed to confirm conjugation of the aptamer with Figure 4Bc shows ac haracteristicp eak at 260 nm, whichc ould be ascribed to the absorption of DNA. [27] For Fe 3 O 4 @COF-Apt-15 (Figure 4Bd diffractionp eaks matched well with those of magnetite( JCPDS no.…”

“…[15,42] TMC (1.325 g) was dissolved in EA (30 mL), and Fe 3 O 4 @SiO 2 @NH 2 (1.0 g) was added to the solution with stirring at 0 8Cf or 1h.A mixture of TMC (1.06 g) and PPD (0.32 g) in EA (30 mL) was added to the resulting solution with stirring at 0 8Cf or 1h.F inally, Fe 3 O 4 @COF-COOH was obtained after reaction at 25 8Cf or 24 h, washed with H 2 Oa nd ethanol, and dried in av acuum oven overnight at 60 8C. [38] Fe 3 O 4 @COF-COOH (1.0 g) was dispersed in PBS (50 mL, pH 7.4) containing EDC (0.8 g) and NHS (1.2 g). Then, the resulting solution was stirred for 30 min to activate the carboxyl groups of Fe 3 O 4 @COF-COOH.…”