A novel beam-splitting microscope tube for taking stereo-pairs with full resolution Nomarski or phase contrast technique, or with epifluorescence

Wolf, Rainer

doi:10.1111/j.1365-2818.1989.tb00558.x

Cited by 8 publications

(6 citation statements)

References 4 publications

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“…A further example of the versatility of anaxial illumination is that it may be extended into a high-resolution, threedimensional imaging system, either by parallel optics or by alternating the illumination aperture (see Inoue. 1986: Wolf, 1989: Teunis et al, 1992.…”

Section: Discussionmentioning

confidence: 99%

Reduced‐carrier single‐sideband microscopy: A powerful method for the observation of transparent microscopical objects

Bretschneider

Teunis

1994

Journal of Microscopy

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Summary The theoretical and practical properties of different forms of contrast formation in the microscope based on anaxial illumination are investigated: so‐called single‐sideband (SSB) techniques. The use of anaxial illumination in transmitted‐light microscopy is by itself a form of phase contrast (asymmetric illumination contrast, or AIC), but needs enhancement via a video circuit coupled to the microscope. The addition of a partially absorbing mask, known as a carrier‐attenuation filter (CAF), in a proper, conjugate plane in the microscope improves contrast substantially. The imaging properties of this reduced‐carrier, single‐sideband imaging method (RC‐SSB)were tested using the transparent parts of a compact disc (CD); the tracks may be treated as small objects with a controllable phase shift. The results were compared both theoretically and experimentally with Zernike phase contrast and with Nomarski differential interference contrast. The SSB technique has been shown to reveal transparent, submicrometre parts of living, unstained tissue, such as microvilli on sensory receptor cells of the transparent catfish, Kryptopterus. The high resolving power, together with the variable spatial‐frequency contrast enhancement, makes it a powerful technique for the imaging of in‐vivo subcellular details.

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Section: Discussionmentioning

confidence: 99%

Reduced‐carrier single‐sideband microscopy: A powerful method for the observation of transparent microscopical objects

Bretschneider

Teunis

1994

Journal of Microscopy

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“…We modified the well-established technique of realtime single lens 3-D microscopy (originally developed for high resolution bright-field, Nomarski or phase contrast optics; [28,29]) to give information about the number and spatial distribution of internal BaSO 4 precipitates. This technique is based on oblique transmitted-light illumination.…”

Section: Real-time 3-d Dark-field Microscopymentioning

confidence: 99%

Real-time 3-D dark-field microscopy for the validation of the cross-linking process of alginate microcapsules

et al. 2005

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“…Since anaxial illumination is equivalent to lateral displacement of the effective condenser aperture, an additional effect is a similar lateral displacement of the image upon defocusing. This property can be used to retrieve the third dimension, depth, in a microscopic image at moderate to high magnifications (Pluta, 1973;Wolf, 1989).…”

Section: Anaxial (Oblique) Illuminationmentioning

confidence: 99%

“…For video recording we used a beam-splitting tube (Wolf, 1989). This device doubles the light beam which leaves the back focal plane of the objective lens, using three prisms, and a glass cube to correct for the difference in optical path length.…”

Section: Stereo Pairs On a Split-screen Video Displaymentioning

confidence: 99%

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Real‐time three‐dimensional tracking of fast‐moving microscopic objects

Teunis¹,

Bretschneider²,

Machemer³

1992

Journal of Microscopy

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SUMMARY Anaxial illumination has been used so far for either enhancement of visual acuity, or three‐dimensional (3‐D) measurements of static objects. Here we report the use of anaxial illumination for 3‐D tracking of fast‐moving objects, with only slight modifications to the standard microscope. To achieve this, we have investigated highspeed recording of stereoscopic images in two different set‐ups.

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A novel beam-splitting microscope tube for taking stereo-pairs with full resolution Nomarski or phase contrast technique, or with epifluorescence

Abstract: SUMMARY A device is presented which for the first time allows stereo‐pairs to be taken with full resolving power on a single frame, since both single views are formed by the complete diffraction image. The device can be introduced into the mononuclear tube of any light microscope.

Cited by 8 publications

References 4 publications

Reduced‐carrier single‐sideband microscopy: A powerful method for the observation of transparent microscopical objects

Reduced‐carrier single‐sideband microscopy: A powerful method for the observation of transparent microscopical objects

Real-time 3-D dark-field microscopy for the validation of the cross-linking process of alginate microcapsules

Real‐time three‐dimensional tracking of fast‐moving microscopic objects

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