Real-time 3-D dark-field microscopy for the validation of the cross-linking process of alginate microcapsules

Wolf, Rainer; Zimmermann, D.; Weber, M.; Feilen, Peter J.; Ehrhart, Friederike; Jungjohann, M. Salinas; Katsen, A.; Behringer, Marcus; Geßner, P.; Pließ, L.; Steinbach, Andrea; Spitz, Jean-Alexis; Vásquez, Julio A.; Schneider, Stephan; Bamberg, Ernst; Weber, Matthias M.; Zimmermann, U.; Zimmermann, Heiko

doi:10.1016/j.biomaterials.2005.04.015

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…Currently, the method of choice for investigating BaSO 4 precipitate distribution is real-time three-dimensional (3-D) dark-field microscopy [33]. This technique gives a true 3-D image at magnifications between 25:1 and 400:1 and requires only a simple microscope with three polarizing or anaglyphic filters and double aperture stops.…”

Section: Microcapsule Technologymentioning

confidence: 99%

Alginate-based encapsulation of cells: Past, present, and future

Zimmermann¹,

Shirley²,

2007

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Replacing dysfunctional endocrine tissues (eg, islets) with healthy, nonautologous material protected against the immune defense of the patient could soon become a reality. Recent advances have resulted in the development of alginate-based microcapsules that meet the demands of biocompatibility, long-term integrity, and function. Focus on the development of good manufacturing practice-conforming microfluidic chip technology for generation of immunoisolated transplants and on cryopreservation technology will bring the cell-based therapy to the market and clinics.

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Section: Microcapsule Technologymentioning

confidence: 99%

Alginate-based encapsulation of cells: Past, present, and future

Zimmermann¹,

Shirley²,

2007

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“…In contrast to the lack of ecological information used to determine fishery regulations, several studies arise since the middle 1980s approaching different aspects of kelp knowledge, such as biology and population ecology [12,13,17,32,33], genetics and taxonomy [18][19][20]34], enhancement and cultivation [35][36][37][38], new and novel uses for their natural by products, for medical nanotechnology, for example (see [39][40][41]).…”

Section: Biological and Ecological Aspectsmentioning

confidence: 99%

The Brown Seaweeds Fishery in Chile

Vásquez¹

2016

Fisheries and Aquaculture in the Modern World

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Chilean fishery of brown algae includes species belonging to the genus Lessonia, Durvillaea, and Macrocystis, which can be found along the coast, ranging latitudes from 18° to 55°S. The exploitation of these seaweeds is done mainly in the Northern coast because the environmental conditions of this region decrease initial production costs. Brown algae are exploited from natural populations and exported to international markets as row material, source of alginates, widely utilized in diverse manufacturing processes and industries. International demand for Chilean kelps has produced sustained increase in harvest during the last decade, reaching more than 390,000 dry tons/year. This chapter approaches the most relevant aspects of the brown seaweed fishery in Chile which covers a wide range of the Southeast Pacific coast, considering the number of commercial species, its abundance and distribution, knowledge achieved on their ecology and biology regarding management, and conservation of these resources, and finally, provides tools for stakeholders and policy makers directed to sustainable management of natural kelp beds occurring in the cold temperate seas.

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“…These important aspects of the fishery are essential to the establishment of regulations that guarantee a sustainable management of this resource. In contrast to the lack of fisheries research, several studies arise in the mid-1980s on the biology and ecology of brown seaweeds (see Santelices and Ojeda 1984;Vásquez and Buschmann 1997;Muñoz et al 2004;Vásquez et al 2006;Graham et al 2007), their genetics and taxonomy (see Macaya and Zuccarello 2010;Tellier et al 2011a, b), enhancement and cultivation (see Vásquez and Tala 1995;Gutiérrez et al 2006;Westermeier et al 2007;Correa et al 2006), and new uses of their natural products (see Wolff et al 2005;Zimmermann et al 2005Zimmermann et al , 2007.…”

Section: Introductionmentioning

confidence: 98%

The Lessonia nigrescens fishery in northern Chile: “how you harvest is more important than how much you harvest”

2012

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In Chile, management of natural resources usually starts right before its imminent collapse or after evident declination. In the northern area of the country, the fishery of brown seaweeds has an enormous social, ecological, and economical importance. More than 11,000 people depend directly or indirectly on the collection and harvesting of this resource. Ecologically, kelps constitute areas for food, reproduction, and refuge for hundreds of invertebrates and fish species. Economically, landings up to 300,000 dry tons per year represent close to US $60 million for the industry. Until 2002, the Chilean brown seaweed fishery was mainly sustained by natural mortality, where plants cast ashore were collected by artisanal fishermen. Since then, three brown seaweed species of economic importance (Lessonia nigrescens, Lessonia trabeculata, and Macrocystis pyrifera) have been intensively harvested in coastal areas between 18°and 32°S. To manage kelp populations along the northern Chilean coast, regulations have been based on the principle "how you harvest is more important than how much you harvest". This exploitation strategy has been adopted in consensus between fishermen, industries, governmental entities, and scientists. Since L. nigrescens represents more than 70% of total brown seaweed landings, this study tests the effects of L. nigrescens harvesting on the following population variables: (1) abundance, (2) distribution, (3) juvenile recruitment, (4) plant morphology, (5) frequency of reproductive plants, and (6) biodiversity of the macroinvertebrate community associated to kelp holdfasts. Our results show that, despite the enormous harvesting pressure on Lessonia density and biomass, the associated macroinvertebrate richness has been maintained, due to normal plant growth and high recruitment all year round.

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Real-time 3-D dark-field microscopy for the validation of the cross-linking process of alginate microcapsules

Cited by 11 publications

References 24 publications

Alginate-based encapsulation of cells: Past, present, and future

Alginate-based encapsulation of cells: Past, present, and future

The Brown Seaweeds Fishery in Chile

The Lessonia nigrescens fishery in northern Chile: “how you harvest is more important than how much you harvest”

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