A novel bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio harveyi

Prasad, Sathish; Morris, P. I.; Hansen, Rasmus; Meaden, Philip G.; Austin, Brian

doi:10.1099/mic.0.28011-0

Cited by 55 publications

(38 citation statements)

References 41 publications

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“…Ekstrak Teripang menunjukkan aktivitas yang lebih tinggi daripada Kanamycin. Hal ini dikarenakan Vibrio harveyi merupakan bateri Gram Negatif yang memiliki Bacteriocins-Like Inhibitor Substances (BLIS), yaitu "harveyicin SY" dengan berat molekul 24 kDA yang berfungsi menghalangi substansi asing dari organisme lain dalam satu strain, antar spesies atau dari lingkungan masuk ke sel [12].…”

Section: Gambar 1 Diameter Daerah Hambat Ekstrak Teripang Terhadap Vunclassified

Aktivitas Antibakteri Ekstrak Teripang Holothuria sp Terhadap Bakteri Vibrio harveyi Secara In vitro

Roihanah

Sukoso

Andayani

2012

JELS

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AbstrakBakteri adalah salah satu penyebab penyakit pada mahluk hidup yang mematikan. Salah satu alternatif yang dapat dilakukan untuk mengatasi masalah tersebut adalah dengan menggunakan Teripang (Holothuria sp.) yang memiliki kandungan bioaktif sebagai bahan antibakteri. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak Teripang terhadap Vibrio harveyi, mempelajari karakter dan struktur bakteri yang dihambat oleh ekstrak bioaktif Teripang dan mempelajari komponen bioaktif yang terkandung dalam Teripang. Hasil uji cakram menunjukkan rerata zona hambat sebesar 8.502 mm sedangkan hasil uji MIC dan MBC menunjukkan bahwa pada dosis 0,55 mg.ml -1 mampu menghambat (Bakteriostatik) dan dosis 0,60 mg.ml -1 mampu membunuh (bakterisidal). Ekstrak kasar Teripang berpengaruh terhadap karakter dan struktur bakteri Vibrio harveyi yaitu dengan merusak dinding sel dan membran sel bakteri. Hasil analisis Spektofometri Infra Merah dari eksktrak teripang dan Ultra Violet dengan pelarut n-heksan serapan diduga mengandung senyawa Triterpenoid. Kata kunci: Aktivitas Antibakteri, Teripang, Vibrio harveyi Abstract Bacteria are one potential deadly cause of disease in organism. One alternative to overcome these problems is to use sea cucumbers (Holothuria sp.) which contain bioactive as antibacteria. Aim of this study was to determine the antibacterial activity of sea cucumber's extracts against Vibrio harveyi, studying the character and bacterial structure which is inhibited by the bioactive extract of sea cucumbers and assess the bioactive components contained in the sea

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Section: Gambar 1 Diameter Daerah Hambat Ekstrak Teripang Terhadap Vunclassified

Aktivitas Antibakteri Ekstrak Teripang Holothuria sp Terhadap Bakteri Vibrio harveyi Secara In vitro

Roihanah

Sukoso

Andayani

2012

JELS

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“…[13][14][15] In this study, the isolate HG-3F, closely related to V. parahaemolyticus (97.6% similarity), showed inhibitory activity only against indicator strains of V. parahaemolyticus and V. splendidus. Moreover, the isolate HG-12F, identified as A. fischeri (100% similarity), showed inhibitory activity only against indicator strains of V. ichthyoenteri, V. parahaemolyticus and V. splendidus.…”

Section: Cfb Group Of Bacteroidetes Actinobacteriamentioning

confidence: 99%

Identification and characterization of bacteria with antibacterial activities isolated from seahorses (Hippocampus guttulatus)

et al. 2010

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Seahorse populations have declined in the last years, largely due to overfishing and habitat destruction. 1 Recent research efforts have, therefore, been focused to provide a better biological knowledge of these species. We have recently studied, as part of the Hippocampus project, wild populations of seahorse (Hippocampus guttulatus) in some areas of the Spanish coast and established breeding programs in captivity. 2 With the development of intensive production methods, it has become apparent that diseases can be a significant limiting factor. Vibrio species are among the most important bacterial pathogens of marine fish. They are responsible for several diseases, and high mortalities due to vibriosis have been reported. 3,4 Thus, we have screened marine bacteria isolated from the intestinal content and cutaneous mucus of seahorses (H. guttulatus) for production of antibacterial compounds against pathogenic Vibrio species. This mechanism of competition offers the possibility of using these antagonistic microorganisms as biological control agents. [5][6][7] Adult seahorses (n¼8) were collected from the coast of Galicia (NW Spain). The culturable microbiota was isolated from the intestinal content and cutaneous mucus as follows. Intestinal content from each seahorse was collected, weighed homogenized using tissue grinders, and vortexed vigorously in sterile saline solution (8.5 g l À1 NaCl), whereas the cutaneous mucus was collected from the dorsal surface with a sterile cotton swab into a small amount of sterile saline solution. Tenfold serial dilutions of samples were prepared and plated on marine agar (Difco, Detroit, MI, USA), tryptic soy agar supplemented with 15 g l À1 NaCl (Cultimed, Barcelona, Spain) and Cytophaga agar prepared with 50% seawater (0.5 g l À1 tryptone, 0.5 g l À1 yeast extract, 0.2 g l À1 sodium acetate, 15 g l À1 agar and adjusted to pH 7.2). All plates were incubated for 3-7 days at 20 1C. Colonies with different morphological characteristics from each sample were selected, subcultured in suitable media and stored in sterile glycerol (15% v/v) at À80 1C.To test the ability of the isolates to inhibit growth of pathogenic Vibrio strains (Table 1), we grew all isolates on suitable agar media at 20 1C for 2-3 days. After incubation, we spotted a loop of each isolate onto the surface of marine agar previously inoculated with overnight cultures of the indicator strain. Clear zones after overnight incubation at 20 1C indicated the presence of antibacterial substances.Bacterial isolates showing antagonistic activity against pathogenic Vibrio strains were identified using the 16S rRNA gene, amplified from extracted genomic DNA with primers 27F and 907R and Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA). 8 PCR (95 1C for 10 min; 30 cycles of 94 1C for 30 s, 50 1C for 1 min and 72 1C for 2 min; and 72 1C for 10 min) yielded products of approximately 0.9 kb, which underwent sequencing. The sequences obtained were compared to those available in the GenBank, EMBL and DDBJ databases with the BLAST p...

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“…Studies on the pathogenic mechanism of V. harveyi have identified a number of virulence-associated factors, notably haemolysin [13], exotoxins [14][15][16][17], bacteriocin-like substance [18], and quorum sensing system, which may participate in pathogenesis by regulating the production of certain proteases and the virulence-associated type III secretion system [19,20]. It is known that some pathogenic V. harveyi strains exhibit higher levels of protease activity in the extracellular products than non-pathogenic strains [6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain

Cheng¹,

Zhang²,

Zhang³

et al. 2010

Vaccine

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A novel bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio harveyi

Cited by 55 publications

References 41 publications

Aktivitas Antibakteri Ekstrak Teripang Holothuria sp Terhadap Bakteri Vibrio harveyi Secara In vitro

Aktivitas Antibakteri Ekstrak Teripang Holothuria sp Terhadap Bakteri Vibrio harveyi Secara In vitro

Identification and characterization of bacteria with antibacterial activities isolated from seahorses (Hippocampus guttulatus)

Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain

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