A novel assay for cobalt-albumin binding and its potential as a marker for myocardial ischemia—a preliminary report

Objective: Oxidative damage may be responsible for the pathogenesis and complications of many diseases. Vitamin D deficiency has been suggested as a potential mediator of various extra-skeletal pathologies. However, there are limited data on anti-oxidant properties of vitamin D. Materials and methods: Forty-one subjects with vitamin D deficiency and 30 healthy controls were enrolled into the study. The levels of total anti-oxidant status (TAS), total oxidant status (TOS), ischemia-modified albumin (IMA), oxidized-low density lipoprotein (ox-LDL), highsensitivity C-reactive protein (hs-CRP) and fibrinogen were measured in both groups. The measurements were repeated in 17 patients after the replacement of vitamin D. Results: Serum IMA and TOS levels were significantly higher (p < 0.001 and p = 0.035, respectively), while TAS levels were significantly lower in patients, compared to controls (p < 0.001). Additionally, fibrinogen was significantly higher in patients than controls (p = 0.003), while ox-LDL and hs-CRP levels were similar between two groups. After the replacement of vitamin D, TAS level significantly increased (p = 0.037), and TOS and fibrinogen levels significantly decreased (p = 0.043 and p = 0.010, respectively). Vitamin D levels were negatively correlated with IMA and fibrinogen levels (r = -0.500, p < 0.001 and r = -0.391, p = 0.002, respectively), although positively correlated with TAS levels (r = 0.430, p < 0.001). No correlation was found between vitamin D levels, and the TOS, ox-LDL and hs-CRP levels. Conclusions: In this study, while serum IMA, TOS and fibrinogen levels were increased, TAS levels were seen to be decreased in patients with vitamin D deficiency. These results suggest that oxidative/anti-oxidative balance shifts in favours of oxidative status in vitamin D deficiency. Arch Endocrinol Metab. 2015;59(4):318-24

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“…IMA was initially proposed as a marker for the diagnosis of myocardial ischemia (10). However, IMA is not a tissue-specific marker of ischemia.…”

Section: Discussionmentioning

confidence: 99%

Serum total oxidant/anti-oxidant status, ischemia-modified albumin and oxidized-low density lipoprotein levels in patients with vitamin D deficiency

Başer

Can

Başer

et al. 2015

Arch. Endocrinol. Metab.

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“…Measurement of the ischemia modified albumin: Reduced cobalt to albumin-binding capacity (IMA level) was measured using the rapid and colorimetric method developed by Bar-Or et al (2000). Briefly, 200 mL of patient serum was transferred into glass tubes and 50 mL of 0.1% CoCl 2 *6H 2 O (Sigma-Aldrich Lot:S38901-248) added.…”

Section: Methodsmentioning

confidence: 99%

Ischemia Modified Albumin Levels and Oxidative Stress in Patients with Bladder Cancer

Elli̇dağ¹,

Eren²,

Aydın³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Impaired oxidative/antioxidative status plays an important role in the pathogenesis of many diseases like cancer. The aim of this study was to evaluate the levels of the novel marker ischemia modified albumin (IMA) and albumin adjusted-IMA (Adj-IMA) in patients with bladder cancer (BC) as well as its association with total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI). Materials and Methods: Forty male patients with BC (mean age, 67.4±12 years) and forty age-sex matched healthy persons (mean age 56.0±1.7 years) were included in this study. Serum levels of IMA, TAS, TOS were analyzed and Adj-IMA and OSI was calculated. Results: Serum IMA, TOS and OSI values were significantly higher in patients with BC compared to controls (p<0.0001, p=0.01 and p=0.01, respectively), whereas TAS was significantly lower in BC patients (p=0.04). There was no significant difference for serum albumin-adjusted IMA levels between groups (p=0.4). Conclusions: In this study, it was found that there was an impaired oxidative/antioxidant status in favor of oxidative stress in BC patients. This observation was not confirmed by Adj-IMA calculation. There is no published report about serum concentrations of IMA in patients with BC. Further studies are needed to establish the relationship of IMA and oxidative stress parameters in BC and the significance of IMA to other cancers.

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“…The IMA level was analyzed colorimetrically by an albumin cobalt binding assay developed by Bar-Or et al, 25 which involves the binding capacity of albumin for Co(II) metal ion. The IMA value was determined by adding fixed amounts of Co(II) to a serum sample and measuring the unbound, free Co(II) with dithiothreitol as the chromogen.…”

Section: Biochemical Analysismentioning

confidence: 99%

Associations of FPG, A1C and disease duration with protein markers of oxidative damage and antioxidative defense in type 2 diabetes and diabetic retinopathy

Reddy

Sethi

Gupta

et al. 2015

Eye

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Purpose To investigate the role of protein oxidative damage and antioxidant defense in relationship to hyperglycemia measured as fasting plasma glucose (FPG), glycated hemoglobin (A1C), and duration of disease in type 2 diabetes mellitus (DM) and diabetic retinopathy (DR). Methods This study recruited 23 nondiabetic subjects, 16 DM patients without any complications and 18 DR patients. The serum ischemia modified albumin (IMA) and glutathione (GSH) levels were measured. The IMA results were corrected for serum albumin. Between-group differences were studied by analysis of variance and betweenvariable associations were studied by Spearman's and partial correlations. Results IMA and cIMA values were elevated, whereas GSH was decreased in both patient groups vs controls (Po0.05), and the increase in IMA formation is not related to serum albumin changes. DR patients have much severe oxidative stress (OS) status with high IMA and cIMA, and low GSH than in the DM group (Po0.05). Both FPG and A1C levels were positively associated with IMA in DM group, while in the DR group, duration of disease too had a positive association with IMA. The antioxidant GSH had negative correlations with FPG (r = − 0.52, P = 0.02) and IMA (r = − 0.49, P = 0.03) in the DR group. Partial correlation analyses predicted mutual or independent associations among parameters. Conclusions Severe OS in DR has been associated with increased FPG, A1C, and disease duration. Both hyperglycemia and elevated oxidative damage detected as IMA are collectively associated with depleted GSH status. Our study unravels the need for monitoring of OS in addition to standard glycemic management in DR.

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A novel assay for cobalt-albumin binding and its potential as a marker for myocardial ischemia—a preliminary report

Cited by 732 publications

References 13 publications

Serum total oxidant/anti-oxidant status, ischemia-modified albumin and oxidized-low density lipoprotein levels in patients with vitamin D deficiency

Serum total oxidant/anti-oxidant status, ischemia-modified albumin and oxidized-low density lipoprotein levels in patients with vitamin D deficiency

Ischemia Modified Albumin Levels and Oxidative Stress in Patients with Bladder Cancer

Associations of FPG, A1C and disease duration with protein markers of oxidative damage and antioxidative defense in type 2 diabetes and diabetic retinopathy

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