A novel aptasensor strategy for protein detection based on G-quadruplex and exonuclease III-aided recycling amplification

N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA

“…The biosensor is highly versatile and can be readily engineered by incorporating appropriate aptamers to detect many disease-related proteins. For example, MUC1, a tumor biomarker highly expressed in human adenocarcinomas, was detected with a detection limit of 3.7 nM [60].…”

Aptamers used for biosensors and targeted therapy

“…The proposed electrochemical aptasensor could detect ATP with a detection limit of 34 pM. Other targets, including cytokines [ 157 ], MUC1 [ 158 ], lysozyme [ 159 ], and ochratoxin A [ 160 ], were also assayed based on Exo III-assisted signal amplification using different design strategies. However, Exo III can only implement the enzymatic activity of digesting the double strand from the 3′-end, so designers must fabricate methods to meet its unique property, restricting its applications to some extent.…”

A fluorometric graphene oxide-based assay for determination of agrB gene transcription in methicillin-resistant Staphylococcus aureus by coupling exonuclease III-assisted target recycling and hybridization chain reaction

“…15 These research studies confirmed that EATR provides a powerful tool for detecting trace molecules. Various enzymes, including polymerases, 16 endonucleases, 17 and exonucleases, 18 have been used to improve sensitivity through advanced isothermal enzymatic processes. Exonuclease III (Exo III) is a nuclease that catalyzes the gradual removal of single nucleotides from the 3 0 -blunt or recessed end of double-stranded DNA (dsDNA) while showing low activity on single-stranded DNA (ssDNA) and 3 0 -protruding termini of dsDNA.…”