A fluorometric graphene oxide-based assay for determination of agrB gene transcription in methicillin-resistant Staphylococcus aureus by coupling exonuclease III-assisted target recycling and hybridization chain reaction

Abstract

Schematic representation of the graphene oxide-based fluorometric bioassay for determination of agrB gene transcription in MRSA by coupling exonuclease III-assisted target recycling and hybridization chain reaction.

Electrochemical aptasensor based on DNA-templated copper nanoparticles and RecJf exonuclease-assisted target recycling for lipopolysaccharide detection

“…In order to continually improve the sensitivity of bioanalytical methods, signal amplification strategies are applied extensively in the designing of various sensors, among which target recycling amplification is the most explored one owing to the associated convenience in designing and operation. 24 Various endonucleases, 25,26 exonucleases, 27,28 nucleases, 29 and metal ion-dependent DNAzymes 30,31 are used as cleavage enzymes in the amplification analysis to enhance the signal/target ratio. RecJf exonuclease is a single-stranded DNA (ssDNA)-specific exonuclease that catalyzes the removal of deoxyribonucleoside monophosphates from the DNA along the 5′ → 3′ direction.…”