A Novel Approach to Turbidimetry of Dense Systems: An Investigation of the Enzymatic Gelation of Casein Micelles

Worning, Peder; Bauer, Rogert; Ōgendal, Lars; Lomholt, Stig B.

doi:10.1006/jcis.1998.5537

Cited by 14 publications

(8 citation statements)

References 14 publications

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“…In fact, in the majority of cases, the sizing method becomes inapplicable before the photon mean free path is much smaller than the path length of the cell. In selected experiments, repeated as a function of cell path length (5 and 2 mm), no significant deviation of path length-scaled turbidity values was observed again indicating that multiple scattering was not a significant problem …”

Section: Resultsmentioning

confidence: 91%

“…Small-angle neutron scattering (SANS) experiments usually show a strong angular dependence for the scattered intensity, typical of the Porod regime, which indicates that the continuous and dispersed phases are separated by sharp interfaces . Time-resolved small-angle light scattering (SALS) can be used to distinguish spinodal decomposition from nucleation and growth mechanisms, , and turbidity measurements have also been used to detect the onset of phase separation. , Although the angular dependence of the scattered light is lost in turbidity experiments by averaging, this technique has several advantages over conventional multiangle light scattering, especially rapidity of the measurements and relative insensitivity to multiple scattering. − In principle, while multiple scattering changes the angular distribution of the scattered intensity, it does not affect the total amount scattered over all angles . However, it should be noted that the existence of a finite acceptance angle in a turbidity measuring instrument means that the possible consequences of multiple scattering should not be dismissed out of hand, and this is discussed again in due course.…”

Section: Introductionmentioning

confidence: 99%

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A Turbidimetric Study of Phase Separating Biopolymer Mixtures during Thermal Ramping

et al. 2000

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A method for particle sizing using a commercial spectrophotometer is presented, based on a Rayleigh-Gans-Debye treatment of the wavelength dependence of turbidity. The finite acceptance angle of the spectrophotometer has been taken into account and shown to be potentially advantageous. The method has been used to monitor the kinetics of droplet growth in phase separating biopolymer mixtures of gelatin and maltodextrin, and predicted droplet sizes have been compared with data obtained from confocal laser scanning microscopy. Furthermore, the temperature dependence of the turbidity data is used to construct a model of the phase behavior of the biopolymer mixture. For systems demixing between 60 and 30 °C, the driving force for phase separation is solely governed by the temperature-dependence of the Flory-Huggins interaction parameters. Below 30 °C the gelatin ordering triggers additional driving forces for phase separation and subsequently leads to the formation of a gel network that traps the system microstructure.

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Section: Resultsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

A Turbidimetric Study of Phase Separating Biopolymer Mixtures during Thermal Ramping

et al. 2000

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“…Simulation studies using different models for particle aggregation have highlighted the fractal nature of the colloidal aggregates obtained . For an object with fractal structure, the expression for Q takes the form: Q = 3 8 ∫ 0 normalπ P ( q , R ) S ( q ) ( 1 + cos 2 θ ) sin θ d θ where S ( q ) is a structure factor that describes the special arrangement of the dispersion elements or monomers within the aggregate…”

Section: Methodsmentioning

confidence: 99%

Colloidal Stability of Bovine Calcium Caseinate Suspensions. Effect of Protein Concentration and the Presence of Sucrose and Lactose

et al. 2010

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Sodium caseinate suspensions (Cas) are stabilized as submicelles in a dynamic system consisting of caseins R, β, and κ. Their use in the food industry is quite widespread because of their functional properties and nutritional value. In the presence of calcium ions, Cas are able to form colloidal stable aggregates in suspension (CCA) at certain Ca 2+ and Cas concentration ratios (Ca:Cas). The aim of this work was to study the effect of the protein concentration on the CCA solubility and viscosity at different Ca:Cas and to relate those two functional properties to protein composition and conformational changes. The stability of CCA was also evaluated in the presence of lactose and sucrose. The apparent solubility of the CCA was determined by applying a spectrophotometric method, and the stability test parameters were obtained using a model based on thermodynamic linked functions. The protein conformational changes were followed by using fluorescence techniques and were related to the partial specific volume and the intrinsic viscosity. The experimental data obtained allowed us to conclude that the stability of CCA depends on the initial protein concentration for the same Ca:Cas ratio. A model based on multiple-step equilibrium was proposed as an approach to explain the stability of Cas against Ca 2+ . Furthermore, it was observed that the addition of Ca 2+ to Cas generates changes in the protein conformation, leading to more compact and symmetrical structures. The colloidal stability of the CCA was favored by the presence of sugars, especially by lactose.

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“…Simulation studies using different models for particle aggregation have highlighted the fractal nature of the colloidal aggregates obtained [31]. For an object with fractal structure, the expression for Q takes the form:…”

Section: Changes In Size and Compactness Degree Of Particlesmentioning

confidence: 99%

A study on bovine kappa-casein aggregation after the enzymatic action of chymosin

Hidalgo

Pires

Risso

2010

Colloids and Surfaces B: Biointerfaces

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A Novel Approach to Turbidimetry of Dense Systems: An Investigation of the Enzymatic Gelation of Casein Micelles

Cited by 14 publications

References 14 publications

A Turbidimetric Study of Phase Separating Biopolymer Mixtures during Thermal Ramping

A Turbidimetric Study of Phase Separating Biopolymer Mixtures during Thermal Ramping

Colloidal Stability of Bovine Calcium Caseinate Suspensions. Effect of Protein Concentration and the Presence of Sucrose and Lactose

A study on bovine kappa-casein aggregation after the enzymatic action of chymosin

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