Ovine caseinate hydrolyzed with Bacillus sp. P7 protease presented antioxidant, antihypertensive, and antimicrobial activities. Hydrolysis time was observed to affect the evaluated bioactivities. Such hydrolysates might have potential applications in the food industry.

The interactions of 1,8-anilinonaphthalenesulfonate (ANS) and Nile Red (NR) with bovine casein micelles (CM) were studied by fluorescence spectroscopy. Both fluorescent hydrophobic markers showed blue shifts of their fluorescence emission picks and fluorescence intensity enhancement, indicating their location in low-polarity regions of CM. Studies at two temperatures showed a weak interaction of high binding capacity (K = 0.031 pM-l at 25 "C) for ANS (marker of anionic nature), probably involving hydrophobic and electrostatic components, and a strong one (K = 36.0 pM-l at 25 "C) for NR (a noncharged molecule), clearly hydrophobic and of lower binding capacity.Comparison with the binding observed on CM disassembled in media without added Ca2+ pointed to the possibility that the markers permeate the porous CM structure acceding to casein molecules located into the micelles. Both markers inhibited CM enzymic coagulation, possibly by occupation of hydrophobic regions on renneted CM, thereby lowering the effectiveness of their collisions. The action of rennet in the first step of coagulation produced a decrease of about 10% of the fluorescence intensity of both bound markers, showing that a fraction of them could be located into the outer hydrophilic stabilizing layer of CM.

No abstract

No abstract

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.