A novel approach to the recovery of biologically active oligosaccharides from milk using a combination of enzymatic treatment and nanofiltration

Sarney, Douglas B.; Hale, Christine; Frankel, Gad; Vulfson, Evgeny N.

doi:10.1002/1097-0290(20000820)69:4<461::aid-bit13>3.0.co;2-l

Cited by 64 publications

(34 citation statements)

References 36 publications

(38 reference statements)

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“…According to the information reported in the literature, pressure does not have a significant effect on the rejection of sugars. 5,6 However, membrane compaction caused by the applied pressure may alter their separation characteristics. The sugar solution filtration experiments were carried out at a constant pressure of 40 bar, and the volume of the initial feed solution was 300 ml in all cases.…”

Section: Methodsmentioning

confidence: 99%

Fractionation of oligosaccharides by nanofiltration

2003

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Two loose nanofiltration membranes (NF-CA-50 and NF-TFC-50) and one dense ultrafiltration membrane (UF-CA-1) were used to fractionate commercial oligosaccharide mixtures by applying diafiltration in a 'dead-end' filtration cell at 40 bar constant pressure with a maximum volume concentration ratio (VCR) of 6 at each fractionation. The rejections of a monosaccharide (glucose) and a disaccharide (lactose) were determined for each membrane; the results indicated that fractionation between these two sugars was possible using the two nanofiltration membranes. During the nanofiltration purification of a commercial oligosaccharide mixture, yields of 19% (w/w) for monosaccharides and 88% (w/w) for di-and oligosaccharides were obtained with the NF-TFC-50 membrane after four filtration steps, indicating that removal of the monosaccharides is possible with only minor losses of the oligosaccharide content of the mixture. The ultrafiltration membrane, at the same time, gave purification levels similar to the NF-TFC-50 membrane with fewer diafiltration steps but with higher losses of di-and oligosaccharides (12% (w/w) for monosaccharides and 53% (w/w) for di-and oligosaccharides on the third run). INTRODUCTIONThe food industry has recently shown increased interest in oligosaccharides as functional food ingredients owing to their proposed beneficial effects on human health. 1 Oligosaccharides are carbohydrates consisting of 2-10 linked monomer sugars and either occur naturally or are produced chemically or by enzymatic transglycosylation or controlled degradation reactions. 2 Oligosaccharides are increasingly included in foods for their prebiotic properties. A prebiotic is defined as a non-digestible food ingredient that beneficially affects the host by selectively stimulating the growth and/or activity of one or more of a limited number of bacteria in the colon (ie Bifidobacterium spp and Lactobacillus spp). 2 Certain oligosaccharides have also been reported to act as soluble dietary fibres and as anticarcinogenic agents. 2 Food-grade oligosaccharides are mixtures containing oligosaccharides of different degrees of polymerisation and monosaccharides. Not all of these sugars possess prebiotic activity and it would be useful to be able to separate the active oligosaccharides from the contaminants. This is particularly important when the materials are to be tested using in vitro models of the human gut, as these models test the fermentation of all carbohydrate material present, whereas in the human situation some components would not reach the colon.

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Section: Methodsmentioning

confidence: 99%

Fractionation of oligosaccharides by nanofiltration

2003

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“…This is a slow method that requires long separation times and has a low capacity. In contrast, two methods have been published for obtaining HMO in large quantities, one using charcoal column chromatography [13], and the other nanofiltration [14]. In each case the milk fat was first removed using centrifugation, and protein precipitated with organic solvents.…”

Section: Introductionmentioning

confidence: 99%

“…However, little methodological information was provided, and the resulting product was not well characterized. Using nanofiltration, Sarney et al [14] isolated HMO from other milk constituents, and compared the resulting HMO produced with gel filtration. Their yield with nanofiltration was 6.7 grams of HMO from 1L of milk, yet there did appear to be residual lactose in the oligosaccharide fraction produced with nanofiltration, but not in that prepared using gel filtration.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Milk Oligosaccharides using Solid-Phase Extraction

Ward¹

2009

TOGLYJ

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Abstract:A method was developed to isolate mono-and disaccharide-free oligosaccharides from human and bovine milk using a combination of enzymatic digestion of lactose and solid-phase extraction. In the initial trial, 2.5 g of oligosaccharides were isolated from one liter of human milk. In subsequent trials this was increased to over 5 g of oligosaccharides per liter. Compared to filtration-based extraction methods, this procedure allows for further isolation of oligosaccharide fractions via modulation of the column rinsing solvent. Neutral monosaccharide composition of the oligosaccharide polymers was investigated using gas chromatographic analysis of the monosaccharides as alditol acetate derivatives. Results indicate oligosaccharides are approximately made up of 24% fucose, 41% galactose, 22% glucose and 13% glucosamine. Isolated bovine and human milk oligosaccharides were compared to lactose as fermentation substrates for Bifidobacterium longum biovar infantis. Lactose fermentation yielded the greatest production of biomass followed by bovine and human milk oligosaccharides.

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“…The earliest reports on the application of membranes for the recovery of oligosaccharides can be traced back to the 1990s (Mok et al 1995;Matsubara et al 1996;Urano et al 1997;Sarney et al 2000). More recently, Goulas et al (2002) reported the nanofiltration on a commercial scale of a galactooligosaccharide mixture and investigated the significance of operating parameters, including pressure, feed concentration and filtration temperature, on the purification process.…”

Section: Primary Purificationmentioning

confidence: 99%