A Novel Approach to Precipitation of Heavy Metals from Industrial Effluents and Single-Ion Solutions Using Bacterial Alkaline Phosphatase

Recent Trends in Phosphatase-Mediated Bioremediation

“…This states that CIAP at alkaline pH from 8 to 11 undergoes slight conformational changes. The enzyme might be attaining kinetically stable conformations at pH 8.5 and 11, as evident from the experimental findings [18]. The effect of substrate concentration on the CIAP activity across a wide range of pH values between 8 and 11 was observed.…”

“…BAP upon hydrolysis in Tris-HCl buffer resulted in higher activity at pH 8.5 till pH 10 at 37°C. The activity of enzyme increased linearly with the increase in substrate concentration [18] (Figure 5). …”

“…When the K m for enzyme catalytic reaction is higher, affinity of the enzyme for its substrate is lower and vice versa. Results showed that the catalytic efficiency at pH 8 is 18,220 ± 2 M −1 s −1 [18].…”

“…Inhibition in enzyme activity was observed at increasing substrate concentrations above1.5 mM pNPP at all pH range except pH 11 where stable activity was noticed. At pH 11, dissociation of pNP from pNPP or transfer of the enzyme to another quaternary state might be highly facilitated [18].…”

“…The activity of the enzyme was quite complex, and possibly all the parameters during enzymatic precipitation studies with respect to incubation time, temperature, pH and substrate concentration have to be studied [18].…”