A novel approach of integrated bioprocessing of cane molasses for production of prebiotic and functional bioproducts

Sharma, Manisha; Patel, Satya Narayan; Lata, Kusum; Singh, Umesh; Krishania, Meena; Sangwan, Rajender S.; Singh, Sudhir P.

doi:10.1016/j.biortech.2016.07.131

Cited by 50 publications

(23 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To the best of our knowledge, the value of °Brix of similar by‐products obtained from citrus fruit has not been reported. Cane and beet molasses were found to have 50.0 and 83.6 °Brix, respectively.…”

Section: Resultsmentioning

confidence: 96%

“…It can be inferred that the OL could be an enriched source of fructose in a similar way to the cane molasses that are used to extract sucrose. Thus, cane molasses contain 200–300 g kg −1 of sucrose, 140–250 g kg −1 of glucose and 150–250 g kg −1 of fructose, whereas OL contains 77.2 g kg −1 of sucrose, 53.3 g kg −1 of glucose and 154.2 g kg −1 of fructose.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Chemical and physicochemical characterization of orange by‐products derived from industry

2018

View full text Add to dashboard Cite

Orange juice waste obtained industrially under the conditions described in the present study could be used as a source of pectic derivatives or fructose in the case of solid or liquid by-products, respectively. The results reported here could diversify the present application of these products as a source of food ingredients, contributing to an improvement in their utilization. © 2018 Society of Chemical Industry.

show abstract

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Chemical and physicochemical characterization of orange by‐products derived from industry

2018

View full text Add to dashboard Cite

show abstract

“…Cane molasses (CM) is generated as a by‐product in sugar industry and consists of sucrose (30–40%, w/v), glucose (5%, w/v), and fructose (5%, w/v; Xu & Xu, ). It has served as a low‐cost feedstock in synthesis of high‐value glucooligosaccharides (Sharma et al, ), isomaltulose (Wu et al, ), and trisaccharide 1‐kestose (Hirabayashi, Kondo, Toyota, & Hayashi, ). Invertase (INV) catalyzes the hydrolysis of sucrose to glucose and fructose.…”

Section: Introductionmentioning

confidence: 99%

Development of food‐grade expression system for d‐allulose 3‐epimerase preparation with tandem isoenzyme genes in Corynebacterium glutamicum and its application in conversion of cane molasses to D‐allulose

Yang

Tian

Zhang

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

D-Allulose 3-epimerase (DAE) has been applied to produce D-allulose, a low-calorie and functional sweetener. In this study, a new DAE from Paenibacillus senegalensis was characterized in Escherichia coli. Furthermore, we presented a tandem isoenzyme gene expression strategy to express multiple DAEs in one cell and construct foodgrade expression systems based on Corynebacterium glutamicum. Seventeen expression cassettes based on three DAE genes from different organisms were constructed.Among all recombinant strains, DAE16 harboring three DAE genes in an expression vector exhibited the highest enzyme activity with 22.7 U/mg. Whole-cell transformation of DAE16 produced 225 g/L D-allulose with a volumetric productivity of 353 g·g −1 ·hr −1 . The catalytic efficiency of strain C-DAE9 integrating total 11 DAE genes in chromosome was 16.4-fold higher than strains carrying one DAE. Fed-batch culture of C-DAE9 gave enzyme activity of 44,700 U/L. We also expressed a thermostable invertase in C. glutamicum and obtained enzyme activity of 29 U/mg. Immobilized cells expressing DAE or invertase exhibited 80% of retained activity after 30 cycles of catalytic reactions. Those immobilized cells were coupled to produce 61.2 g/L D-allulose from cane molasses in a two-step reaction process. This study provided an efficient approach for enzyme preparation and allowed access to produce D-allulose from other abundant and low-cost feedstock enriched with sucrose.

show abstract

“…Besides FOS production from molasses, other bioactive compounds have been produced using molasses as fermentation media, such as: isomaltulose (using un-pretreated sugar cane molasses) (Wu et al, 2017) and levan type fructans (using sugar beet molasses, starch molasses, sugar cane molasses and sugar cane syrup) (Küçüka, Kazak, & Güney, 2011;Roberto, Oliveira, Rui, Antonia, & Colabone, 2007). Sharma et al (2016) evaluated a bioprocess using cane molasses as substrate with the strain L. mesenteroides to produce oligosaccharides (OS), in which successfully produced OS of DP3-DP6, approximately 124 g/kg of fresh molasses. Also, a functional monosaccharide D-psicose was produced by epimerization of D-Fructose.…”

Section: Molassesmentioning

confidence: 99%

Fructooligosaccharides production from agro-wastes as alternative low-cost source

Rosa

Flores‐Gallegos

Muñiz‐Márquez

et al. 2019

Trends in Food Science & Technology

View full text Add to dashboard Cite

Background: The prebiotic properties of fructooligosaccharides (FOS) are well documented. The high demand of functional food by the food, pharmaceutical and biotechnology industries have lead researchers to explore new and more feasible processes to produce FOS. Not only economical substrates are being exploited to reduce costs, but also, seeking to attend a global problem, the excessive generation of agro-industrial wastes that are polluting the earth, which are not being completely exploited, have been a concern. Scope and approach: The purpose of this review is to present a concise (but wide-ranging) appraisal on the latest advances in fructooligosaccharides production from agro-wastes, as alternative low-cost source. Emphasis is placed on the examination, analysis and discussion of the prospects for using different agro-industrial waste bioresources for the production of FOS and FOS-producing enzymes. Key findings and conclusions: The food, agro-industrial and forestry industries generate large volumes of waste, that are mainly composed of complex carbohydrates and crude proteins, that can be useful as nutrients for microbial growth, and enzymes or other metabolites production. Agro-industrial wastes are discarded, and its accumulation generates a severe environmental impact. The development of value-added processes using agroindustrial wastes is very attractive and becomes an environmentally friendly waste management method.

show abstract

A novel approach of integrated bioprocessing of cane molasses for production of prebiotic and functional bioproducts

Cited by 50 publications

References 39 publications

Chemical and physicochemical characterization of orange by‐products derived from industry

Chemical and physicochemical characterization of orange by‐products derived from industry

Development of food‐grade expression system for d‐allulose 3‐epimerase preparation with tandem isoenzyme genes in Corynebacterium glutamicum and its application in conversion of cane molasses to D‐allulose

Fructooligosaccharides production from agro-wastes as alternative low-cost source

Contact Info

Product

Resources

About