A novel approach for propagation of recalcitrant pistachio cultivars that sidesteps rooting by ex vitro grafting of tissue cultured shoot tips

Marín, Juan Antonio Antolinos; García, Eva de; Lorente, P.; Andreu, P.; Arbeloa, A.

doi:10.1007/s11240-015-0871-7

Cited by 16 publications

(20 citation statements)

References 40 publications

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“…The presented data confirmed that 1/2 MS media supplemented with IBA during 60 days had the best significant effect on the shoot elongation. Phloroglucinol (PG) is a phenolic compound that inhibits auxin oxidation and stimulates shoot and root growth (Pérez et al 2015;Marín et al 2016). The results of this study show a synergistic effect of phloroglucinol with IBA, an analog of auxin, which can be degraded by decarboxylation as reported by De Klerk et al 2011.…”

Section: Discussionsupporting

confidence: 64%

Optimizing micropropagation of drought resistant Pyrus boissieriana Buhse

Zakavi

Askari

Irvani

2016

Physiol Mol Biol Plants

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The present study concentrated on introducing a micropropagation protocol for a drought resistant genotype from , which is the second most naturally widespread pear species in Iran with proper physiological and medicinal properties. Proliferating microshoot cultures were obtained by placing nodal segments on MS medium supplemented with BAP and IBA or NAA. The highest number of shoots (27 shoots per explant) were obtained with 1.5 mg l BAP and 0.05 mg l IBA, but this combination did not produce shoots of desirable length (>1.7 cm). Combination of 1.75 mg l BAP and 0.07 mg l IBA was the best for the shoot multiplication in with a sufficient number of shoot production (22.33 shoots per explant) and relatively more appropriate shoot length. The larger and greenish leaves were obtained when PG was added to the best multiplication treatment. Microshoot elongation was carried out in 1/2 and 1/4 MS medium containing 50-100 mg l PG with different concentrations of IBA or NAA at intervals of 30-60 days. Significant increase in shoot length was detected after 45-60 days of culture in the presence of PG. The highest shoot length (8 cm) was recorded on 1/2 MS medium supplemented with 0.5 mg l IBA and 100 mg l PG. GA negatively affected number and length of shoots and generally caused generation of red leaves. The highest percentage of root induction (100%) and root length (9 cm) were obtained on 1/6 strength MS medium supplemented with 0.005 mg l IBA. All plantlets were hardened when transferred to ex vitro conditions through a period of 25-30 days. The results suggest axillary shoot proliferation of could successfully be employed for propagation of candidate drought resistant seedling.

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Section: Discussionsupporting

confidence: 64%

Optimizing micropropagation of drought resistant Pyrus boissieriana Buhse

Zakavi

Askari

Irvani

2016

Physiol Mol Biol Plants

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“…This contrasting result might be explained by a different sensitivity to PGRs (type and concentration) offered by different genotypes or by carry-over effects from the multiplication phase, or else by a combination of both the factors. Indeed, in vitro rooting is considered genotype-specific within many woody fruit species such as almond [32], pistachio [33], and peach [34], among the others. On the other hand, Keresa et al [25] evaluated in vitro caper rooting efficiency as affected by the carry-over effect and evoked the role of CKs as auxin antagonists and their involvement in tuning auxin transport and biosynthesis.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Rooting of Capparis spinosa L. as Affected by Genotype and by the Proliferation Method Adopted During the Multiplication Phase

Gianguzzi

Barone

Sottile

2020

Plants

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The in vitro rooting of three caper (Capparis spinosa L.) selected biotypes, grown in a commercial orchard on the Sicilian island of Salina (38°33′49” N), was performed using—as base material for rooting experiments—shoot explants proceeding from two different in vitro culture systems: solid medium and liquid culture in a PlantForm bioreactor (TIS). The regenerated shoots of each accession were submitted to different auxin treatments (NAA, IBA, IAA - 1 or 2 mg L−1; NAA+IBA 0.75 and 0.25 mg L−1, respectively), supplemented with sucrose or fructose (mg L−1). The highest rooting rate in terms of root percentage (67%) was reached with the explants of the selected accession ‘Sal 39’ proceeding from liquid culture in PlantForm and induced in the MS medium with sucrose, as a carbon source, supplemented with NAA 0.75 mg L−1 + IBA 0.25 mg L−1, after six days in a climatic growth chamber at 25 ± 1 °C in the dark and then placed under a cool white fluorescent lamp, with a PPFD of 35 μmol m−1 s−1 and a photoperiod of 16 h. On the other hand, poor rooting rate was generally achieved under all the tested experimental conditions with the other biotypes, ‘Sal 37’ and ‘Sal 35’, demonstrating the strong role exerted by the previously adopted proliferation method and by the genotype for successful caper in vitro rooting.

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“…The occurrence of physiological disorders during micropropagation of certain species of Pistacia has been attributed to several causes as the culture media composition, the PGRs or the culture system 26,34,37,53–55 , but none of the studies focus on determining the causes of those disorders. We have paid attention to the physiological abnormalities that occurred during two independent micropropagation experiments of Pistacia carried out in our laboratory, recording and categorizing data related to several disorders to get insight about the causality of their appearance.…”

Section: Discussionmentioning

confidence: 99%

“…The vitamins-mixtures of original MS 10 , Gamborg B5 64 or DKW 31 are commonly added to the components of culture media used for micropropagation of Pistacia species 26,35,37,54,55,65 . The use of computer-based neurofuzzy logic has revealed a linear positive impact of pyridoxine-HCl and nicotinic-acid on promoting some shoot multiplication parameters of pistachio 52 , but, as far as we know, there is no demonstrative conclusion about the influence of vitamin on the physiological disorders occurred during in vitro culture of other woody species.…”

Section: Discussionmentioning

confidence: 99%

Computer-based tools provide new insight into the key factors that cause physiological disorders of pistachio rootstocks cultured in vitro

Nezami-Alanagh

Garoosi

Landín

et al. 2019

Sci Rep

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During the in vitro culture of plants some physiological disorders caused major problems that have been associated with culture media composition. The objective of this study was to better understand the abnormal physiological response of two pistachio rootstocks to changes in culture media ingredients. On this purpose, two computer-based tools were employed: design of experiment (DOE) and neurofuzzy logic. DOE was employed to generate a five-dimensional IV-design space allowing to reduce the number of treatments from 6,250 to 61. The second one, an artificial intelligence (AI) tool, neurofuzzy logic, was used to understand the cause-effect relationships between the factors studied (25) and seven physiological disorders including shoot-tip necrosis (STN), leaf necrosis (LN), leaf color (LC), basal callus (BC) formation, shoot fasciation (SF), hyperhydricity and epinasty, typically described during pistachio in vitro culture. Four out of the seven disorders were successfully modeled, being significantly affected by a limited number of factors. STN and BC were significantly affected by the concentration of EDTA − . However, while a low concentration of EDTA − reduces the STN, promotes BC. LN and LC were strongly alleviated by high amounts of thiamine-HCl. Undoubtedly, the results demonstrate the importance of recording and using data related to physiological disorders along with growth parameters when developing suitable culture media for plant tissues. The computer-based tools have been useful to: i) well sample experimental design; ii) reduce the final number of treatments and the experimental work; iii) identify the key factors affecting each disorder; iv) get insight about the causes that promote the appearance of physiological disorders. Our findings demonstrate that the recently AI designed POM media, although not optimal, is the most suitable (favouring growth and limiting physiological abnormalities) media for in vitro culture of pistachio compared to those media, currently used.

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A novel approach for propagation of recalcitrant pistachio cultivars that sidesteps rooting by ex vitro grafting of tissue cultured shoot tips

Cited by 16 publications

References 40 publications

Optimizing micropropagation of drought resistant Pyrus boissieriana Buhse

Optimizing micropropagation of drought resistant Pyrus boissieriana Buhse

In Vitro Rooting of Capparis spinosa L. as Affected by Genotype and by the Proliferation Method Adopted During the Multiplication Phase

Computer-based tools provide new insight into the key factors that cause physiological disorders of pistachio rootstocks cultured in vitro

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