A novel approach for an immunogen against Corynebacterium pseudotuberculosis infection: An Escherichia coli bacterin expressing phospholipase D

Pinho, Rodrigo Barros de; Silva, Mara Thais de Oliveira; Brenner, Gabriel; Alves, Mirna Samara Dié; Azevedo, Vasco; Portela, Ricardo Dias; Borsuk, Sibele

doi:10.1016/j.micpath.2021.104746

Cited by 7 publications

(1 citation statement)

References 33 publications

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“…Furthermore, it should be noted that even with 10% survival in the G1 and G2 controls, there was no statistically significant difference for G5, which showed 100% death. The fact of having survival in some animals in the control group has already been demonstrated in other studies with vaccines for CLA as in the study of Pinho et al (2021) [ 21 ] where the control group also had 10% survival.…”

Section: Resultssupporting

confidence: 52%

Peptide vaccines designed with the aid of immunoinformatic against Caseous Lymphadenitis promotes humoral and cellular response induction in mice

Droppa-Almeida¹,

Silva²,

Gaspar³

et al. 2021

PLoS ONE

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Caseous Lymphadenitis (CLA) is a chronic disease that affects also small ruminants. CLA is caused by Corynebacterium pseudotuberculosis and is responsible for high economic losses due to the formation of superficial and visceral granulomas, the latter is considered as asymptomatic CLA causing high levels of dissemination. Several vaccination strategies, in which the use of synthetic peptides stands out. Thus, this work aimed to evaluate the protective potential of peptide vaccines designed to determine the immunodominant epitopes of CP40 against CLA in mice. The animals were divided into eight groups separated in controls (G1—PBS, G2—Saponin and G9—rCP40) and experimental (G3—pep1, G4- pep2, G5-pep3, G6-pep4, G7-pep5 and G8-pep6), these were vaccinated on days 0 and 15 by a subcutaneous route. 60 days after the first immunization, all animals were challenged with C. pseudotuberculosis. On days 0, 15, 60, and 120 after the first immunization, blood samples were taken to measure immunoglobulins. On the same day of the challenge, the splenocytes were isolated and assayed for the production of IL-2, IL-4, IL-6, IFN-γ, TNF-α, IL-17, and IL-10. After vaccinations, the animals were challenged and all of them were affected by the disease which led to their death. The G6 and G8 groups provided 10% protection and the G7 provided 20%. The G3 and G4 groups provided 30% and 40% protection respectively. The peptides showed the production of Total IgG antibodies and cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α), indicating a possible activation of the Th1 type response. However, groups G3, G5, G6, and G8 showed production of IL-17. None of the study groups showed IL-10 production. The immunogenicity of the peptides was not enough to protect these animals and it is believed that the use of adjuvants based on PAMPs may improve the immune response offered by these peptides.

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Section: Resultssupporting

confidence: 52%