A novel apoptosis‐inducing protein from <i>Helicobacter pylori</i>

Shibayama, Keigo; Kamachi, Kazunari; Nagata, Noriyo; Yagi, Tetsuya; Nada, Toshi; Doi, Yohei; Shibata, Naohiro; Yokoyama, Keiko; Yamane, Kunikazu; Kato, Haru; Iinuma, Yoshitsugu; Arakawa, Yoshichika

doi:10.1046/j.1365-2958.2003.03305.x

Cited by 93 publications

(90 citation statements)

References 32 publications

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“…6). Although both spots were only weakly antigenic in our immunoblots (11) the protein is known to be a virulence-and apoptosis-inducing factor of H. pylori that occurs in the form of two fragments (25,26). Additionally, it was hypothesized that the protein is membrane-associated (25), and here the first 36 amino acids were not covered in the PMFs so that a cleavage of a signal sequence might have occurred.…”

Section: Spot Nomentioning

confidence: 88%

Analysis of Automatically Generated Peptide Mass Fingerprints of Cellular Proteins and Antigens from Helicobacter pylori 26695 Separated by Two-dimensional Electrophoresis

Krah

Schmidt

Becher

et al. 2003

Molecular & Cellular Proteomics

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Helicobacter pylori is a causative agent of severe diseases of the gastric tract ranging from chronic gastritis to gastric cancer. Cellular proteins of H. pylori were separated by high resolution two-dimensional gel electrophoresis. A dataset of 384 spots was automatically picked, digested, spotted, and analyzed by matrix-assisted laser desorption ionization mass spectrometry peptide mass fingerprint in triple replicates. This procedure resulted in 960 evaluable mass spectra. Using a new version of our data analysis software MS-Screener we improved identification and tested reliability of automatically generated data by comparing with manually produced data. Antigenic proteins from H. pylori are candidates for vaccines and diagnostic tests. Previous immunoproteomics studies of our group revealed antigen candidates, and 24 of them were now closely analyzed using the MS-Screener software. Only in three spots minor components were found that may have influenced their antigenicities. These findings affirm the value of immunoproteomics as a hypothesis-free approach. Additionally, the protein species distribution of the known antigen GroEL was investigated, dimers of the protein alkyl hydroperoxide reductase were found, and the fragmentation of ␥-glutamyltranspeptidase was demonstrated.

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Section: Spot Nomentioning

confidence: 88%

Analysis of Automatically Generated Peptide Mass Fingerprints of Cellular Proteins and Antigens from Helicobacter pylori 26695 Separated by Two-dimensional Electrophoresis

Krah

Schmidt

Becher

et al. 2003

Molecular & Cellular Proteomics

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“…H. felis and H. suis induce apoptosis in the murine gastric epithelial cell line GSM06 and in the human gastric adenocarcinoma cell line AGS (unpublished results). Gamma-glutamyltranspeptidase, which has been associated with the induction of apoptosis in gastric epithelial cells and the inhibition of T-cell proliferation by H. pylori (125,195,201), is also produced by gastric non-H. pylori Helicobacter species (Table 2).…”

Section: Virulence Factors Of Gastric Non-h Pylori Helicobacter Speciesmentioning

confidence: 99%

Gastric Helicobacters in Domestic Animals and Nonhuman Primates and Their Significance for Human Health

et al. 2009

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SUMMARY Helicobacters other than Helicobacter pylori have been associated with gastritis, gastric ulcers, and gastric mucosa-associated lymphoid tissue lymphoma in humans. These very fastidious microorganisms with a typical large spiral-shaped morphology were provisionally designated “H. heilmannii,” but in fact they comprise at least five different Helicobacter species, all of which are known to colonize the gastric mucosa of animals. H. suis, which has been isolated from the stomachs of pigs, is the most prevalent gastric non-H. pylori Helicobacter species in humans. Other gastric non-H. pylori helicobacters colonizing the human stomach are H. felis, H. salomonis, H. bizzozeronii, and the still-uncultivable “Candidatus Helicobacter heilmannii.” These microorganisms are often detected in the stomachs of dogs and cats. “Candidatus Helicobacter bovis” is highly prevalent in the abomasums of cattle but has only occasionally been detected in the stomachs of humans. There are clear indications that gastric non-H. pylori Helicobacter infections in humans originate from animals, and it is likely that transmission to humans occurs through direct contact. Little is known about the virulence factors of these microorganisms. The recent successes with in vitro isolation of non-H. pylori helicobacters from domestic animals open new perspectives for studying these microorganisms and their interactions with the host.

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“…Ultrathin (80-nm) sections of tissue fixed in phosphatebuffered saline (PBS) containing 2% paraformaldehyde and 2.5% glutaraldehyde were postfixed with 2% osmium tetroxide and embedded in Epon resin. Sections were stained with uranyl acetate and lead acetate and examined using a JEM-1220 electron microscope at 80 kV (Jeol Ltd., Tokyo, Japan) as described previously (57).…”

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confidence: 99%

Genome Organization and Pathogenicity ofCorynebacterium diphtheriaeC7(−) and PW8 Strains

Iwaki¹,

Komiya²,

Yamamoto³

et al. 2010

Infect Immun

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Corynebacterium diphtheriae is the causative agent of diphtheria. In 2003, the complete genomic nucleotide sequence of an isolate (NCTC13129) from a large outbreak in the former Soviet Union was published, in which the presence of 13 putative pathogenicity islands (PAIs) was demonstrated. In contrast, earlier work on diphtheria mainly employed the C7(؊) strain for genetic analysis; therefore, current knowledge of the molecular genetics of the bacterium is limited to that strain. However, genomic information on the NCTC13129 strain has scarcely been compared to strain C7(؊). Another important C. diphtheriae strain is Park-Williams no. 8 (PW8), which has been the only major strain used in toxoid vaccine production and for which genomic information also is not available. Here, we show by comparative genomic hybridization that at least 37 regions from the reference genome, including 11 of the 13 PAIs, are considered to be absent in the C7(؊) genome. Despite this, the C7(؊) strain still retained signs of pathogenicity, showing a degree of adhesion to Detroit 562 cells, as well as the formation of and persistence in abscesses in animal skin comparable to that of the NCTC13129 strain. In contrast, the PW8 strain, suggested to lack 14 genomic regions, including 3 PAIs, exhibited more reduced signs of pathogenicity. These results, together with great diversity in the presence of the 37 genomic regions among various C. diphtheriae strains shown by PCR analyses, suggest great heterogeneity of this pathogen, not only in genome organization, but also in pathogenicity.

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A novel apoptosis‐inducing protein from Helicobacter pylori

Cited by 93 publications

References 32 publications

Analysis of Automatically Generated Peptide Mass Fingerprints of Cellular Proteins and Antigens from Helicobacter pylori 26695 Separated by Two-dimensional Electrophoresis

Analysis of Automatically Generated Peptide Mass Fingerprints of Cellular Proteins and Antigens from Helicobacter pylori 26695 Separated by Two-dimensional Electrophoresis

Gastric Helicobacters in Domestic Animals and Nonhuman Primates and Their Significance for Human Health

Genome Organization and Pathogenicity ofCorynebacterium diphtheriaeC7(−) and PW8 Strains

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