A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from a marine Chlorella ellipsoidea and its antihypertensive effect in spontaneously hypertensive rats

A novel peptide purified from the fermented microalga Pavlova lutheri attenuates oxidative stress and melanogenesis in B16F10 melanoma cells

“…Microalgae have been popular food stuff worldwide, and there are rich in protein, minerals, carbohydrates, and bioactive substance [2]. Therefore, it is thought that microalgae could be developed as useful functional materials.…”

“…Interest has emerged to identify and characterize bioactive peptides from microalgae proteins [2,30]. These bioactive peptides can be derived by fermentation of microalgae proteins.…”

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“…In this experiment, the <5 kDa molecular weight fractions showed a better inhibition rate. It has been previously reported (Ko et al, 2012) that a marine Chlorella ellipsoidea hydrolysate was fractionated into three fractions (>10, 5-10, and <5 kDa) using UF and the <5 kDa fraction exhibited strongest ACE inhibition activity and had an IC50 value of 0.89±0.04 mg/mL .It is possible to understand that low molecular weight fractions had more potent bioactivity than that of the high molecular weight fractions. Gel filtration chromatography further separates and purifies the hydrolysates and gives efficient activity.…”

“…Ultrafiltration (UF) membrane technology has been successfully utilized to obtain powerful protein hydrolysate fractions, which could avoid the loss of bioactive substances during treatment (Ko et al, 2012;Wu, Jiang, Jing, Zheng, & Yan, 2016). In view of this, in order to obtain fractions with better α-AI activity, a peptic hydrolysate of commercial dried laver protein fractionated with UF membrane in to different molecular weight cut-offs.…”

“…In addition, the antihypertensive, Angiotensinconverting enzyme (ACE) inhibiting properties and antioxidant activities of seaweed protein hydrolysates and peptides has been reported in the previous studies (Beaulieu, Sirois, & Tamigneaux, 2016;Ko et al, 2012;Qu et al, 2015;Qu et al, 2010;. Proteins that inhibit α-amylases have also been isolated from plants and microorganisms (Svensson, Fukuda, Nielsen, & Bonsager, 2004).…”